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991.
992.
A Scientific Working Group on DNA Analysis Methods (SWGDAM) developmental validation study was carried out on two Y-STR multiplex systems (MPI and MPII) that collectively permit the co-amplification of 19 Y-STR markers, including DYS393, DYS392, DYS391, DYS389I, DYS389II, Y-GATA-A7.2 (DYS461), DYS438, DYS385a and DYS385b (MPI); DYS425, DYS388, DYS390, DYS439, DYS434, DYS437, Y-GATA-C.4, Y-GATA-A7.1 (DYS460), Y-GATA-H.4, and DYS 19 (MPII). Performance checks subsequent to PCR parameter optimization indicated that MPI and MPII were suitably reproducible, precise and accurate for forensic use. The sensitivity of the systems was such that a full 19-locus Y-STR profile was obtainable with 150-200 pg of male DNA, and some loci were detectable even with as little as 20-30 pg of input DNA. Primate specificity was demonstrated by the lack of cross-reactivity with a variety of commonly encountered bacterial and animal species, with the single exception of a monomorphic canine product that was outside of the size range of human alleles from any of the 19 loci. Not surprisingly, cross-reactivity was observed with a number of male and female nonhuman primates. Environmentally compromised samples produced full or partial Y-STR profiles. For example, a semen stain exposed to the outdoor elements for six months still gave a 13-locus Y-STR profile. Although a limited number of female DNA artifacts were observed in mixed stains in which the male DNA comprised 1/300 of the total, the full 19-locus male profile was easily discernible. Even at a 1500-to-2000-fold dilution of male DNA with female DNA partial Y-STR profiles were obtained. Furthermore, the potential utility of MPI and MPII for forensic casework is exemplified by their ability to dissect out the male haplotype in a variety of case-type samples, including, inter alia, post-coital vaginal swabs, admixed male and female bloodstains, the nonsperm fraction from a differentially extracted semen stain, and determination of the number of male donors in mixed semen stains. 相似文献
993.
994.
Objective. Three competing explanations for the distributionof Japan's ODA in Asia are empirically examined in this paper.The first explanation hypothesizes that Japan reacts to US pressureand interests as it formulates its foreign aid policy. The competingexplanations argue that Japanese ODA is used to promote Japan'snational economic interests or humanitarian goals. Methods.We examine the determinants of Japanese ODA in 14 Asian countriesfor the period of 19791998. The effects of the independentvariables are estimated using ordinary least squares (OLS) withpanel-corrected standard errors. Results. US strategic interestswere found to have no effect on aid disbursements for the periodin question. Rather, we find that Japan's national economicinterests have shaped Japanese aid decisions in Asia. The disbursementpattern of Japanese ODA is also influenced by poverty in therecipient country. Employing measures from the Political TerrorScale, Freedom House, and Polity IV, we find no effect for democracyor human rights. 相似文献
995.
996.
997.
This article analyses Japanese policy towards the Middle Eastin the post-Cold War era. The article argues that Japanese policyhas begun to move beyond the reactive diplomacy of the ColdWar years. The focus of this new approach has been Japan's growingcontribution to soft security in the region. However,Japan retains a tendency to focus on its narrow interests withoutfully taking into account the broader strategic consequencesof its policies. Yet, in order to protect its overall interestsin the region, Japan needs to recognize the diminishing utilityof this free rider approach and adopt a more activerole regarding hard security issues. Even allowingfor the domestic constraints on Japanese policy, there is muchJapan can do in this regard, especially in coordination withthe US. 相似文献
998.
999.
MDMA ("ecstasy") has gained renewed popularity as a drug of abuse. To access the epidemiology and causes of death of MDMA-positive fatalities, all deaths investigated by the OCME that tested positive for MDMA (22 deaths) between January 1997 and June 2000 were reviewed. There were three deaths in each 1997 and 1998, eleven in 1999, and five in the first part of 2000. Of these 22 deaths, 13 were due to acute drug intoxications, 7 due to mechanical injury (blunt trauma, gunshot wounds), and 2 due to a combination of natural disease and acute drug intoxication. Evidence of recent opiate and/or cocaine use was found in 7 of the acute intoxication deaths and in none of the traumatic or combination natural/intoxication deaths. The race of all decedents was White between the ages of 17-41 years, and 18 of 22 were men. 相似文献
1000.
Interpreting small quantities of DNA: the hierarchy of propositions and the use of Bayesian networks
The dramatic increase in the sensitivity of DNA profiling systems that has occurred over recent years has led to the need to address a wider range of interpretational problems in forensic science. The issues surrounding questions of the kind "whose DNA is this?" have been the subject of considerable controversy but now it is clear that the emphasis is shifting to questions of the kind "how did this DNA get here?" Such issues are discussed in this paper and new insights are provided by two particular recent developments. First, the notion of the "hierarchy of propositions" that has arisen from a project called Case Assessment and Interpretation (CAI) that has been running in the British Forensic Science Service (FSS). Second, a technique for drawing inferences in the face of many interacting considerations, known as "Bayesian networks"--or "Bayes' nets" for short--that has been the subject of an earlier paper in this journal (1). The discussion is carried out by means of case studies, based on actual cases. It is clear that, whereas the inference in relation to the source of the DNA in a crime sample might be overwhelmingly strong, the inference in relation to the propositions that a jury must consider relating to the identity of the actual offender may be much more tentative. 相似文献