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531.
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Fernández-Mestre M Alfonso-Sánchez MA Gendzekhadze K Layrisse Z Peña JA 《Journal of forensic sciences》2006,51(3):703-704
POPULATIONS: Whole blood samples from 74 unrelated healthy individuals were collected. The donors' sample included Venezuelan mestizos from various regions of the country, but mostly from the resident population of Caracas City. A Venezuelan mestizo is the offspring of a mating between a native Venezuelan and a person born in Europe, mainly in Spain. 相似文献
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Crespillo M Paredes MR Prieto L Montesino M Salas A Albarran C Alvarez-Iglesias V Amorin A Berniell-Lee G Brehm A Carril JC Corach D Cuevas N Di Lonardo AM Doutremepuich C Espinheira RM Espinoza M Gómez F González A Hernández A Hidalgo M Jimenez M Leite FP López AM López-Soto M Lorente JA Pagano S Palacio AM Pestano JJ Pinheiro MF Raimondi E Ramón MM Tovar F Vidal-Rioja L Vide MC Whittle MR Yunis JJ Garcia-Hirschfel J 《Forensic science international》2006,160(2-3):157-167
We report here a review of the seventh mitochondrial DNA (mtDNA) exercise undertaken by the Spanish and Portuguese working group (GEP) of the International Society for Forensic Genetics (ISFG) corresponding to the period 2003-2004. Five reference bloodstains from five donors (M1-M5), a mixed stain of saliva and semen (M6), and a hair sample (M7) were submitted to each participating laboratory for nuclear DNA (nDNA; autosomal STR and Y-STR) and mtDNA analysis. Laboratories were asked to investigate the contributors of samples M6 and M7 among the reference donors (M1-M5). A total of 34 laboratories reported total or partial mtDNA sequence data from both, the reference bloodstains (M1-M5) and the hair sample (M7) concluding a match between mtDNA profiles of M5 and M7. Autosomal STR and Y-STR profiling was the preferred strategy to investigate the contributors of the semen/saliva mixture (M6). Nuclear DNA profiles were consistent with a mixture of saliva from the donor (female) of M4 and semen from donor M5, being the semen (XY) profile the dominant component of the mixture. Strikingly, and in contradiction to the nuclear DNA analysis, mtDNA sequencing results yield a more simple result: only the saliva contribution (M4) was detected, either after preferential lysis or after complete DNA digestion. Some labs provided with several explanations for this finding and carried out additional experiments to explain this apparent contradictory result. The results pointed to the existence of different relative amounts of nuclear and mtDNAs in saliva and semen. We conclude that this circumstance could strongly influence the interpretation of the mtDNA evidence in unbalanced mixtures and in consequence lead to false exclusions. During the GEP-ISFG annual conference a validation study was planned to progress in the interpretation of mtDNA from different mixtures. 相似文献
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José G. Albors 《The Journal of Technology Transfer》2002,27(3):263-273
The ceramic tile cluster industry in Spain has actually a global turnover of approximately 3.000 M Euros of which a 60% is exported. Its annual growth has been around a steady 20% the last five tears. In spite of the traditional Italian technology leadership, the clusterization of this industry in Spain (90% of the producers are located in a 40 km geographical circle) has altered these traditional R&D roles. The increasing rate of technology transfer and co-operation, which is taking place, especially in the product development area, has enabled this change. This paper will aim to analyze the cluster competitiveness and the technology formal and informal cooperation networks, which have been established during the last twenty years in the industry and especially in the enamelling and decoration sub sector. A critical variable relates to product and design development. An outstanding fact has been the technology demand-pull movement exerted from the producers (200 firms mostly SMEs) and the consequent quick product development cycles observed in the latest sectoral fairs. 相似文献
538.
Takaaki Shinomiya Maurice Muller Pierre H. Muller René Lesage 《Forensic science international》1978,12(2):157-163
Conclusive evidence was obtained in this study that immunoelectrophoresis could be used in the identification of blood-stains in two particular cases: for diagnosis or approach to diagnosis of the age of a blood-stain, and for diagnosis of the human origin of a blood-stain that had been treated with petroleum products.Although many aspects should be investigated in more detail, it is certain that this method can be employed in combination with other methods. 相似文献
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