Trichophyton Mentagrophytes Perforates Hair of Adult Corpses in the Gaseous Period*

Abstract: Despite the substantial literature on mycology, there are still limited reports of the interaction between fungi and human hosts in the postmortem period. Thus, the main goal of this study was to investigate the in vitro perforation test using Trichophyton mentagrophytes on hair from adult corpses in the postmortem period (gaseous period). The protocol was carried out with positive (prepubescent children’s hair) and negative controls (healthy adult hair) as well. One strain of Trichophyton rubrum was also used as a negative perforation control. Perforations were found in all the hair samples from corpses and prepubescent children after 12–14 days exposure to T. mentagrophytes and were absent in the hair samples of healthy adults. Furthermore, hair perforation was not observed with T. rubrum. Our preliminary findings suggest the use of T. mentagrophytes as a potential marker of the death interval in forensic science.     

Diel Activity and Effect of Carcass Decomposition on the Attractiveness to the Forensically Important Species Oxelytrum Discicolle (Coleoptera: Silphidae)†

Oxelytrum discicolle is a carrion beetle commonly found in the Neotropical region, mainly associated with cadavers and carcasses. Information on behavior related to the likelihood of cadaver colonization is scarce. We performed two field experiments in peri‐urban forest in Brazil in order to strengthen our knowledge on O. discicolle behavior. In the first experiment, we assessed the preference for piglet carcasses at different stages of decomposition offered simultaneously in two seasons, dry and rainy, while in the second experiment we investigated the diel activity of adults. A total of 493 adults were collected in the experiments. A significantly higher abundance was detected in the rainy season. There was a marked preference for carcasses at advanced stages. Adults exhibited a typical nocturnal behavior, the number trapped at night was 17 times greater than those collected during the day. Oxelytrum discicolle is nocturnal species that prefers carcasses in advanced stage of decomposition.     

Risk terrain modeling for road safety: identifying crash-related environmental factors in the province of Cádiz,Spain

Environmental Criminology has developed a robust framework that provides the scientific support and necessary foundation for crime analysis through crime mapping. This theoretical approach focuses on the situational and temporal characteristics of criminal opportunity rather than on the offender’s behavior for crime prevention. In the scope of road safety and traffic crashes, few studies have adopted this approach. This study used risk terrain modeling (RTM), developed by the Rutgers Center on Public Security, to determine the relative importance of varying environmental risk factors on alcohol-related crashes and traffic accidents. The independent variables consisted of a set of potential environmental risk factors, while the dependent variable comprised all DWI crashes and traffic accidents in the province of Cádiz in 2012. According to the results of the current study, restaurant locations are spatially associated with the occurrence of drunk driving crashes, while proximity restaurants and recreational lodging centers correlate with the sites of traffic accidents.     

Eyewitnesses: A comparison of live,video, and photo line-ups

An experiment was conducted in which the effectiveness of three line-up methods (live, video, and photographs) was compared. Participants witnessed a staged event and were subsequently required, by one of the three methods, to identify the target. Both target-present and target-absent line-ups were used. The results showed that all methods led to an equal number of correct identifications (hits). In the target-absent line-ups, participants more often incorrectly identified a person in the video and photographs condition than in the live condition. Presenting photographs simultaneously or sequentially did not affect the number of correct judgments. Dynamic video images, showing locomotion and facial expression, had a marginal significant effect on the number of correct judgments as compared with the static video images. Practical implications are discussed.     

The 2000-2001 GEP-ISFG Collaborative Exercise on mtDNA: assessing the cause of unsuccessful mtDNA PCR amplification of hair shaft samples

We report the results of Spanish and Portuguese working group (GEP) of International Society of Forensic Genetics (ISFG) Collaborative Exercise 2001-2002 on mitochondrial DNA (mtDNA) analysis. 64 laboratories from Spain, Portugal and several Latin-American countries participated in this quality control exercise. Five samples were sent to the participating laboratories, four blood stains (M1-M4) and a sample (M5) consisting of two hair shaft fragments. M4 was non-human (Felis catus) in origin; therefore, the capacity of the labs to identify the biological source of this sample was an integral part of the exercise. Some labs detected the non-human origin of M4 by carrying out immuno-diffussion techniques using antihuman serum, whereas others identified the specific animal origin by testing the sample against a set of animal antibodies or by means of the analysis of mtDNA regions (Cyt-b, 12S, and 16S genes). The results of the other three human blood stains (M1-M3) improved in relation to the last Collaborative Exercises but those related to hairs yielded a low rate of success which clearly contrasts with previous results. As a consequence of this, some labs performed additional analysis showing that the origin of this low efficiency was not the presence of inhibitors, but the low quantity of DNA present in these specific hair samples and the degradation.As a general conclusion the results emphasize the need of external proficiency testing as part of the accreditation procedure for the labs performing mtDNA analysis in forensic casework.     

Results of the GEP-ISFG collaborative study on the Y chromosome STRs GATA A10, GATA C4, GATA H4, DYS437, DYS438, DYS439, DYS460 and DYS461: population data

The Spanish and Portuguese ISFG Working Group (GEP-ISFG) carried out a collaborative exercise in order to asses the performance of two Y chromosome STR tetraplexes, which include the loci DYS461, GATA C4, DYS437 and DYS438 (GEPY I), and DYS460, GATA A10, GATA H4 and DYS439 (GEPY II). The groups that reported correct results in all the systems were also asked to analyse a population sample in order to evaluate the informative content of these STRs in different populations. A total of 1020 males out of 13 population samples from Argentina, Brazil, Costa Rica, Macao, Mozambique, Portugal and Spain were analysed for all the loci included in the present study. Haplotype and allele frequencies of these eight Y-STRs were estimated in all samples. The lowest haplotype diversity was found in the Lara (Argentina) population (95.44%) and the highest (99.90%) in Macao (China). Pairwise haplotype analysis showed the relative homogeneity of the Iberian origin samples, in accordance with what was previously found in the European populations for other Y-STR haplotypes (http://www.ystr.org). As expected, the four non-Caucasian samples, Macao (Chinese), Mozambique (Africans), Costa Rica (Africans) and Argentina (Lara, Amerindians), show highly significant Phist values in the pairwise comparisons with all the Caucasian samples.     

Mix-Ups, Mistake and Moral Judgement: Recent Developments in U.K. Law on Assisted Conception

Hard cases make bad law. In a matter of months, two such cases involving assisted reproduction have appeared before the U.K. High Court and legislation has been enacted. The common threads between them are consent and fatherhood. The first case concerns a ‘mistake’ resulting in sperm from the wrong man being used to create an embryo for a couple and the second the revocation of consent by a man to his former partner being allowed to use an embryo they created together. Furthermore, Parliament has intervened, passing legislation which sets out when sperm from a dead man may be used by his former partner to generate an embryo. This note argues that the three developments cannot be reconciled with one another and that the cases in particular, decided on a narrow legal basis, convey contradictory messages, leading in at least one instance to a gross injustice.