GC/MS衍生化测定精浆中游离支链氨基酸和果糖

目的 建立人精浆中支链氨基酸和果糖的气相质谱衍生化分析方法.方法 精液样本液化离心后,按精子活率的临床标准分为正常组和非正常组,经TMS微波衍生化,GC/MS分析两组支链氨基酸、果糖衍生物含量差异.结果 正常组与非正常组的缬氨酸,亮氨酸,异亮氨酸在相对含量上均有一定差异.正常组3种氨基酸相对含量高于非正常组.果糖分析观察到同样的特征,正常组相对含量高于非正常组.结论 精子活率低的精浆样本仍可检出支链氨基酸和果糖,其差异有助于无精子、少精子精斑的法医学鉴别.     

Comparison of two methods for isolating DNA from human skeletal remains for STR analysis

Abstract: The quality and efficiency of a standard organic DNA isolation method and a silica‐based method using the QIAGEN Blood Maxi Kit were compared to obtain human DNA and short tandem repeats (STRs) profiles from 39 exhumed bone samples for paternity testing. DNA samples were quantified by real‐time PCR, and STR profiles were obtained using the AmpFlSTR^® Identifiler^® PCR amplification kit. Overall, the silica‐based method recovered less DNA ranging from 0 to 147.7 ng/g (average 7.57 ng/g, median = 1.3 ng/g) than did the organic method ranging from 0 to 605 ng/g (average 44.27 ng/g, median = 5.8 ng/g). Complete profiles (16/16 loci tested) were obtained from 37/39 samples (95%) using the organic method and from 9/39 samples (23%) with the silica‐based method. Compared with a standard organic DNA isolation method, our results indicate that the published silica‐based method does not improve neither the quality nor the quantity of DNA for STR profiling.     

A study of the evaporation of a solvent from a solution--application to writing ink aging

When writing ink is placed on a substrate, a drying process begins. This process is dependent on the composition of the ink and of the substrate. Lociciro et al. provide an equation that describes the drying process based on models developed by earlier investigators. The work given here develops an equation for the drying process that is based on a different and rather simple model. This model considers the evaporation of a solution in an opened vertical container (e.g., a beaker) and consists of a volatile, non-hygroscopic solvent with a non-volatile solute dissolved in it. Three assumptions are made: (a) the rate of evaporation is proportional to the vapor pressure of the solution and to the solution's exposed surface area, (b) this solution vapor pressure is proportional to the solvent vapor pressure with the proportionality constant being the solvent mole fraction (Raoult's law), and (c) a small fraction of the solvent remains trapped in the solute after evaporation ceases. What results is a differential equation, which, when solved, gives the solvent weight W(t) as an implicit function. What emerges naturally from this treatment is the fact that the function W(t) can have a point of maximum acceleration. Prior to this point the drying process is fast and after this point, the drying process is slow. An approximation to W(t) is taken to be the sum of two exponential functions, one describing the fast drying region and the second describing the subsequent slow drying region. Upon including an additive constant, this approximation turns out to be similar to, but not the same as that provided by Lociciro et al. However, their equivalence is shown and then tested using the two inks examined by Lociciro et al. (the drying of a Bic and a Staedtler blue ballpoint ink). The examples of (solvent+solute) systems ("inks") given here consist of the solvent (2-phenoxyethanol) and a solute such as a dye (crystal violet) or a polymer resin such as synthetic resin SK or polyvinyl butyral. In the case of polymers, the Raoult's law assumption is replaced with one developed by the Flory-Huggins theory of solutions. Saturation and film formation, both of which slow the evaporation rate, are also discussed.     

Traumatic bilateral vertebral artery dissection

Traumatic vertebral artery dissection is not often seen by forensic pathologists, and cases investigated are scarce in the forensic literature. We present the case of a 40-year-old woman cyclist who was struck by a car while wearing a helmet, and was neurologically near normal immediately thereafter at Emergency. She presented 48 h later with acute right hemiparesis, decreasing level of consciousness, and unsteadiness. CT revealed massive cerebellar infarction. CT angiography was normal. The patient died in coma 7 days after injury and autopsy revealed bilateral edematous cerebellar infarction and bilateral vertebral artery dissection. Rotational neck injury and mural tear in the wall of the Atlantic parts of both vertebral arteries is suggested as the possible mechanism of the arterial injury. Head and neck injuries are reported as a precipitating cause of vertebral artery injury. The possible influence of trauma may be further underestimated if longer intervals between vessel dissection and ischemia occur. The current case illustrates that "talk-and-die" syndrome may be due to occult vertebral artery dissection, possibly bilateral. In forensic cases of delayed death after mild trauma to the head and neck, the vertebral arteries should be examined for the cause of death.     

非婚生子女认领制度比较研究——兼及立法建议

非婚生子女认领制度是亲子法领域确立非婚生子女与其生父母法律身份的一项重要制度.我国现行<婚姻法>对非婚生子女的法律地位予以明确界定,非婚生子女与婚生子女受到法律的同等保护,但确认亲子身份的认领制度却付之阙如,这不仅使亲子法的立法结构及内容存在缺陷,而且实践中通过公证办理亲子认领的做法无法可依.学界对这一问题缺乏必要的关注和深入的探讨,也使之成为立法中一个较为薄弱的环节.在我国,以2010年制定民法典为目标,正在对各项制度进行纵深研究.认领制度作为民法典的一个细部结构,中国民法典婚姻家庭编之法学专家建议稿已对此表示了一种倾向,就子女认领制度作出了规定,但规定过于原则.     

Procedural justice,minorities, and religiosity

The current research explores six hypotheses derived from the well-known procedural justice-based model of legitimacy in two different religious groups in Israel, and adds to the model the effect of religiosity on the perceived legitimacy of rules and institutions of social control. Our results, based on data from a representative sample of 1,216 Israeli Jews and Arabs, provide general support for the hypotheses. We found that the social order is perceived as less legitimate by the Arab minority compared with the Jewish majority, and by highly religious members of the Jewish majority compared with those who are less religious.     

A free market bailout alternative?

It has been more than 3 years since the collapse of the investment bank Lehman Brothers and the beginning of the Troubled Asset Relief Program. Most recently, the sovereign debt crisis in Europe has led to the bailout of the governments of Ireland, Portugal and Greece. A main reason behind these bailouts is to support European banks loaded with government bonds on their balance sheet. In this article we analyze the detrimental consequences of the public bailout in 2008 and argue that a free market alternative existed. The alternative of a private bailout outlined in this article, consisting of the conversion of liabilities into equity and a private capital increase, largely avoids the problems of a public bailout. Similarly, a public bailout of governments of the Eurozone to sustain banks may be detrimental.     

Politicization,banking experience and risk in savings banks

During the global financial crisis, criticism of the politicization and lack of professionalization of the savings banks has taken a central position in the political debate. The aim of this article is to analyze if the political presence of governing bodies in Spanish savings banks has been reflected in their various risk-taking behaviors before and during the financial crisis. We will also analyze whether the influence of the chairman’s banking experience matters. The results do not provide evidence that the composition of the boards of savings bank, or even their politicization, have played a role. However we show that savings banks run by a chairman with previous banking experience are likely to be significantly more solvent and less volatile.     

当前我国营养医师处方权范围问题研究

营养医师是临床上为住院患者开展营养咨询、诊断、监测、调配饮食并协助临床疾病治疗的营养科在职医师。营养医师作为"医师"本应拥有营养药品处方权,但实践中由于营养医师的类型不同,导致了相应处方权范围的差异。为更好地服务临床,建议设置过渡期、严格营养医师转岗条件,修改临床营养师考试内容,在临床医学和中医学下开设营养学专业。