Saliva identification in forensic samples by automated microextraction and intact mass analysis of statherin

The enzyme α-amylase has long been a commonly targeted protein in serological tests for saliva. While being especially abundant in saliva, α-amylase is detectable in vaginal secretions, sweat, fecal matter, breast milk and other matrices. As a result, assays for α-amylase only provide a presumptive indication of saliva. The availability of mass spectrometry-based tools for the detection of less abundant, but more specific, protein targets (e.g., human statherin) has enabled the development of high confidence assays for human saliva. Sample throughput, however, has traditionally been low due to multi-step workflows for protein extraction, quantitation, enzymatic digestion, solid phase cleanup, and nano-/capillary-based chromatography. Here, we present two novel “direct” single-stage extraction strategies for sample preparation. These feature immunoaffinity purification and reversed-phase solid-phase microextraction in conjunction with intact mass analysis of human statherin for saliva identification. Mass analysis was performed on the Thermo Scientific Q-Exactive™ Orbitrap mass spectrometer with a 10-min analytical run time. Data analysis was performed using Byos^® from Protein Metrics. Two sample sets were analyzed with a population of 20 individuals to evaluate detection reliability. A series of casework-type samples were then assayed to evaluate performance in an authentic forensic context. Statherin was confidently identified in 92% and 71% of samples extracted using the immunoaffinity purification and solid phase microextraction approaches, respectively. Overall, immunoaffinity purification outperformed the solid phase microextraction, especially with complex mixtures. In toto, robotic extraction and intact mass spectrometry enable the reliable identification of trace human saliva in a variety of sample types.     

Using knowledge for control in fragmented policy arenas

Analysts and researchers typically value knowledge for its contributions to the wisdom of policy action. Policymakers, however, typically value knowledge for its contributions to the exercise of political control. Our research on flows of knowledge among federal, state, and local education agencies documents how knowledge may increase the effectiveness of various control strategies. In these cases, knowledge is not intended to enhance rationality; it may or may not. It is intended to enhance control, and it does. Knowledge for control is particularly useful to policymakers who seek to intervene in policy arenas characterized by fragmented authority and widely dispersed resources.     

The Course and Psychosocial Correlates of Personality Disorder Symptoms in Adolescence: Erikson's Developmental Theory Revisited

Personality disorder symptoms were investigated in a community sample of young people (n = 714) to assess their relationship over time with well-being during adolescence and the emergence of intimacy in early adulthood. Drawing on Erikson's theory of psychosocial development, changes in adolescent well-being were conceptualized as indirect indicators of identity consolidation. Cluster B personality disorder symptoms (borderline, histrionic, and narcissistic symptoms) were conceptualized to represent identity diffusion—i.e. maladaptive personality traits that usually resolve during the identity crisis of adolescence. Latent growth models were used in 2 age cohorts to assess (1) interrelationships between Cluster B symptoms, well-being, and intimacy at mean ages 13.8 and 18.6 years; and (2) associations between their developmental trajectories over the next 6 years. As expected, higher personality disorder symptoms were associated with lower well-being during adolescence, and declines in personality disorder symptoms over time were associated with corresponding gains in well-being. Consistent with Erikson's developmental theory, there was an inverse relationship between Cluster B symptoms and intimacy that increased in strength as young people entered adulthood. As an indicator of successful identity consolidation, well-being was significantly associated with intimacy in female adolescents and young adults.     

Becoming better health care providers: outcomes of a primary care service-learning project in medical school

Medical educators have begun to embrace service-learning as a method for teaching medical students to be more socially responsible, patient-oriented practitioners. However, research documenting the learning outcomes of service-learning in medical education is limited. In this paper, written documents generated through evaluation of a mandatory, structured community service-learning experience were analyzed qualitatively to discover the diverse learning outcomes among 24 students who participated in the experience. Preliminary findings indicate that students developed skills and attitudes directly related to competencies of concern in most U.S. medical programs. These preliminary findings may help other programs articulate learning outcomes for their service-learning programs. Further, these preliminary findings may stimulate more systematic research (qualitative and quantitative) in this area.     

Effectiveness of state mandates to maintain local government health services for the poor

State governments have used several types of mandates to assure that local governments fulfill state-defined responsibilities, including public health care for the poor. This article reports the findings of a study of procedural public-participation mandates and financial sanctions imposed by California to hold counties to their obligations to provide indigent health care. An inventory of the laws' implementation in all California counties found noncompliance by some counties, although all counties complied after a state Health Services Department unit was established to monitor the counties and provide them with state aid. Case studies in eight counties identified factors that influenced the effectiveness of the laws in modifying or reversing county proposals to close county hospitals or reduce other health services. Policy guidelines are suggested for states that want to develop mandates to enforce indigent-care responsibilities of local governments.