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21.
Volume Contents
Contents of Volume 19 (2000) 相似文献22.
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Reliable, sensitive, rapid and quantitative enzyme-based assay for gamma-hydroxybutyric acid (GHB) 总被引:1,自引:0,他引:1
Several assays for gamma-hydroxybutyrate (4-hydroxybutyrate, GHB) have been developed based on the enzyme gamma-hydroxybutyrate dehydrogenase (GHB-DH). Enzymatic oxidation of GHB by NAD+ is coupled to diaphorase-mediated reduction of pro-dye to yield colored product. GHB-DH from Ralstonia eutropha was cloned and expressed as a stable fusion protein easily purified by affinity chromatography. Quantitative initial velocity and endpoint versions of the assay in solution are described. Michaelis-Menten parameters for oxidation of GHB and ethanol were estimated. A semi-quantitative "dipstick" version of the assay on paper also is described. Both solution endpoint and "dipstick" assays are sensitive to about 0.05 mg GHB/mL using 10 microL of sample. Ethanol at concentrations possible in urine and agents used to stabilize physiological fluids for forensics analysis do not interfere significantly. The "dipstick" assay also allows detection of GHB in alcoholic beverages after evaporation of about one-fourth drop of beverage before testing. The enzymatic assay for GHB is reliable, sensitive, inexpensive and rapid. 相似文献
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Drug Enforcement Administration 《Federal register》2003,68(194):57799-57804
This regulation implements the new threshold requirements and mail order reporting requirements of the Methamphetamine Anti-Proliferation Act of 2000 (MAPA), which was enacted on October 17, 2000. DEA is amending its regulations to reduce the thresholds for pseudoephedrine and phenylpropanolamine for retail distributors and for distributors required to submit mail order reports. Also, DEA is amending its regulations to require mail order reports for certain export transactions. DEA is codifying exemptions from the mail order reporting requirements for certain distributions to nonregulated persons and certain export transactions. This rule is consistent with the intent of MAPA to prevent the diversion of drug products to the clandestine manufacture of methamphetamine and amphetamine, and simultaneously reduce the industry reporting burden. 相似文献
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Thirty-six male students, drawn from a sample of 1195, were interviewed to obtain a personal history. A battery of projective psychological tests (Rorschach and TATs) were also administered to them. The students were divided into four groups of nine each, Jewish radicals (JR), Christian radicals (CR), Jewish moderates (JM), and Christian moderates (CM), to test the significance of religious background as it related to political outlook. Eight significant psychological variables were found and defined. No differences were found between JMs and CMs. Radicals differed from moderates on three variables: negative identity, masochistic surrender, and treating people as concepts. In addition, JR subjects demonstrated consistently a wandering fantasy, flight from the mother, the mother as salient, and machismo as psychological variables. CRs were not characterized by any of these variables. As with both groups of moderates, the father of the CRs was psychologically salient, but unlike the moderates, CTs perceived their fathers as flawed. The possible dynamic meaning of these configurations is discussed, as are their possible relationship to radical behavior and radical political ideology.This study was supported by grants from the American Jewish Committee and The National Science Foundation (GS35307A).Director of Resident Education, McLean Hospital. M.D., Harvard Medical School; residency training at Boston Veteran's Administration Hospital and Beth Israel Hospital in psychiatry. Psychoanalytic training, Boston Psychoanalytic Institute. Major interest: depression.Director of Training for Psychology Interns. Ph.D., Brandeis, 1960; Master's degree, University of Illinois. Major interest: schizophrenia.Professor of Political Science, Smith College. Major interest: modern European history — applying psychoanalytic methods to historical and social problems like student activism. 相似文献