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71.
Deoxyribonucleic acid (DNA) typing of ribosomal ribonucleic acid (rRNA) genes was performed with a polymerase chain reaction (PCR) assay for species identification. A variable region of the 28S ribosomal RNA gene was amplified with primers complementary to flanking sequences phylogenetically well conserved. The products of twelve animal DNAs (human, Japanese monkey, dog, cattle, pig, cat, rabbit, mouse, rat, chicken, frog, and fish) were separated by polyacrylamide gel electrophoresis, each revealing a few bands ranging from 150 to 100 base pairs. The band patterns obtained from each DNA sample differed in number and size, which indicates the applicability of the method to species identification. Samples containing either as little as 1 pg of DNA or degraded DNA of 0.2 to 0.5 kb in length were able to give detectable bands. Postmortem human tissue DNAs were tested as an example. They showed a pattern identical to the human control one, which was distinct from those of the other animals examined. 相似文献
72.
O. M. Laleye Victor Ayeni 《International Journal of Politics, Culture, and Society》1993,6(4):555-571
Conclusion We have in this paper tried to set out a framework which can facilitate analysis of the current and future situation of traditional rulership in contemporary African society. Our presentation of a proposed model for such an analysis is no doubt still in its elementary stages of formation. Much more work needs to be done. Nevertheless, our analysis has revealed certain salient elements and uncovered certain unresolved issues. If traditional rulers genuinely care about their own future they will have to devise techniques of legitimation which not only endear them to the government and the non-traditional elites, but also, and more importantly, to the mass of the people. True, the original role of the traditional rulers may be no more; still, it may be possible to salvage what is left. 相似文献
73.
N C Martin A A Pirie L V Ford C L Callaghan K McTurk D Lucy D G Scrimger 《Science & justice》2006,46(3):179-184
In the forensic science laboratory, the recovery of spermatozoa from vaginal swabs, or vaginal cells from penile swabs, can help determine if sexual intercourse may have taken place. There are several methods used to recover spermatozoa and cells from the swabs before visualisation on a microscope slide and most of these methods use water. Phosphate buffered saline (PBS) is a non-toxic solution used in many biological laboratories. Unlike water, PBS prevents cells rupturing or shrivelling up due to osmosis. This study demonstrates that PBS can be used for the extraction of spermatozoa and cells from swabs and that PBS does not affect subsequent DNA profiling. 相似文献
74.
The 2003 Duma election resulted in the victory of the party of power, thereby strengthening managed democracy in Russia. Since then, political trends in Russia's regions and rural politics provide considerable evidence of the increasing ability of pro-Kremlin forces to maximise their electoral fortunes in national contests. These political trends make it likely that United Russia will not only emerge as the dominant party in the 2007 Duma election, but will provide the Kremlin and Putin's successor with a compliant lower house. The outcome is that Putin's successor will find the political infrastructure to continue managed democracy. 相似文献
75.
Netherlands International Law Review - 相似文献
76.
Das im November 2005 in Kraft getretene und kürzlich punktuell ge?nderte Projekt-Mechanismen-Gesetz bildet die gesetzliche
Grundlage für die Beteiligung der Bundesrepublik Deutschland an in- und ausl?ndischen Klimaschutzprojekten im Rahmen der projektbezogenen
flexiblen Mechanismen des Kyoto-Protokolls. Mit ihm sollen Unternehmen aus dem In- und Ausland zur „Anmeldung“ ihrer Klimaschutzprojekte
in Deutschland gewonnen werden. Sowohl die grunds?tzliche Verabschiedung des Gesetzes als auch die genannte Motivation sind
zu begrü?en. Dennoch ist eine Reihe von ?nderungen weiterhin erforderlich. 相似文献
77.
78.
K Lalu P J Karhunen P Rautiainen 《The American journal of forensic medicine and pathology》1992,13(3):196-198
A 6-month-old girl died suddenly without any previous symptoms of heart failure. Autopsy examination showed cardiomegaly (97 g) with a severely fibrotized myocardium. The left coronary artery was originating from the pulmonary artery. Histologically, the myocardium showed myocardial infarcts of different ages, as well as grossly thickened arterial branches due to increased flow in left-right shunt. We suggest that rare anomalies of the coronary arteries should be considered in the autopsies of suspected sudden infant death syndrome cases. 相似文献
79.
K. Michael Reynolds Pamala L. Griset Ernest Scott 《American Journal of Criminal Justice》2006,31(1):1-17
Many attempts have been made over the last several decades to improve communication among law enforcement agencies. This article
is a case study of a “low-level” data sharing project in Florida that could serve as a national model. The Florida Law Enforcement
Data Sharing Consortium is a partnership between the University of Central Florida and more than one hundred law enforcement
agencies. It offers an inexpensive, yet technically advanced alternative to the proprietary data sharing model. Its distributed
architecture was endorsed by the Markle Foundation, the 9/11 Commission, and the 2004 National Security Act. Civil liberties
concerns raised by this and other types of data sharing projects are discussed. 相似文献
80.
C. Haedrich C. Ortmann O.R. Hofmann J. Seilwinder A. Klein 《Forensic Science International Supplement Series》2006,160(2-3):173-177
In a case of suicidal application of electricity differences between the rectal temperature of the body and the suspected time of death were observed.In order to answer the question whether an electric current from hand to hand over >30 min led to a rise in body temperature FEM-based computer simulations and animal experiments were carried out. Both resulted in a warming of the soft parts in the arm without warming the body core. Thus a temperature-based estimation of the time since death can also be used in cases with electricity as the cause of death. Besides, in the animal experiment we found a spontaneous rise in the body core temperature even without application of electricity which may be a reason for the typical temperature plateau after death. 相似文献