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Analysis of length polymorphisms at STR loci in the human genome has become a standard approach for comparative genotyping in many areas including disease research and diagnostics, parentage assessment, investigations of human diversity, and forensic science. The simultaneous analysis of multiple STR loci through multiplex PCR and multicolor fluorescence detection offers sample conservation, high throughput, and automated genetic analysis. Careful design and optimization of tetranucleotide STR multiplexes has led to reliable, standardized systems that powerfully differentiate and distinguish individual human DNA profiles. The development of these multiplex systems involved a rigorous experimental strategy that included careful selection of PCR primer sequences (for yield, specificity, and multiplex compatability), along with optimization of PCR component concentrations, thermal cycling parameters, and fluorescence detection conditions. This developmental approach rendered well-characterized DNA typing systems that are high performing (sensitive, specific, and balanced), optimized to universal parameters (same reaction conditions), resilient to fluctuations in reaction conditions, and simple to implement and use routinely. 相似文献
123.
James I. Walsh 《政策研究评论》2002,19(1):80-106
This article evaluates the role of increased capital mobility, sectoral interests, and domestic institutions in bringing about policy change in French capital markets. Capital mobility played an indirect role by making it more costly for French governments to pursue inflationary economic policies. But it was domestic politics, not capital mobility, that led governments to achieve lower inflation by stabilizing the exchange rate. The key domestic political factor was institutional change to regulatory practices, while financial markets reduced bank lending to industry and internationalized French finance, breaking the strong ties and comon monetary diplomacy interests of bankers, industralists, and policymakers, and thereby weaken the political priority of promoting domestic growth and industrial competitiveness. 相似文献
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Walsh SJ Triggs CM Curran JM Cullen JR Buckleton JS 《Journal of forensic sciences》2003,48(5):1091-1093
Well constructed sub-population databases are fundamental to the application of DNA-based forensic statistics. The size of such databases can affect the ability to examine adequately statistical or population genetic features, and the integrity of both the DNA profile and associated ethnicity information is also of importance. Use of short tandem repeat (STR) DNA profiling technology and the thoughtful construction of the governing legislation has seen large databases of DNA profiles collated for the four major sub-populations of New Zealand. Examination of the data illustrates the suitability of self-declaration as a means of categorizing samples on the basis of ethnicity. 相似文献
126.
This article discusses the principal recent developments in,and future prospects for, constitutional change, judicial review,fiscal arrangements, local government, public policy, and intergovernmentalrelations since the 1977 Publius issue on Australian federalism,taking into account the arguments presented in the other contributionsto this volume. It highlights the continuing vertical imbalanceof Australian fiscal federalism, and the Hawke Labor government'srecent initiative for a closer working partnership with thestates for reforming intergovernmental arrangements. It arguesthat while there is scope for the latter, there is greater needfor the former. Nevertheless, the 1980s was significant as adecade of predominantly Labor governments working with the federalConstitution, while the 1990s promises to be a decade of celebratingthe centenary of the Australian Constitution and working towardimproving the functioning of its federal system. 相似文献
127.
Dr Sinead Walsh 《Development in Practice》2016,26(6):706-718
While NGO accountability is widely discussed in the literature, there has been little work done on how accountability systems operate in practice. This article aims to address this gap by using qualitative empirical data to provide a case study of one NGO’s initiative in this area. ActionAid has made substantial, high-profile efforts to improve its downward accountability since 2000. Using data from fieldwork conducted in Uganda and internationally, the article reveals the operational obstacles which have hindered ActionAid in its attempts to strengthen its accountability to intended beneficiaries at the community level, and reviews ActionAid’s ongoing commitment to this goal. 相似文献
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Jennifer J. Muehlenkamp Barent W. Walsh Moira McDade 《Journal of youth and adolescence》2010,39(3):306-314
Non-suicidal self-injury (NSSI) continues to be a problem among youth and there is a great need for programming aimed at reducing
NSSI in adolescents. The signs of self-injury program is the first known NSSI school-based prevention program for adolescents
that attempts to increase knowledge, improve help-seeking attitudes and behaviors, and decrease acts of NSSI. A total of five
schools implemented the program in selected classrooms (n = 274 adolescents; 51.5% female, mean age = 16.07 years) that consisted of predominantly Caucasian (73%) adolescents. Researchers
collected pre-post evaluation surveys of the program and feasibility interviews were conducted with the school guidance personnel
who ran the program. Results indicated the prevention program did not produce iatrogenic effects, increased accurate knowledge
and improved help-seeking attitudes and intentions among students. No significant changes were found in regards to self-reported
formal help-seeking actions. Feasibility responses indicate the program is user-friendly and well received by school personnel.
The data offer preliminary evidence that the program may be an effective prevention program for schools. 相似文献
130.