Real-time forensic DNA analysis at a crime scene using a portable microchip analyzer

An integrated lab-on-a-chip system has been developed and successfully utilized for real-time forensic short tandem repeat (STR) analysis. The microdevice comprises a 160-nL polymerase chain reaction reactor with an on-chip heater and a temperature sensor for thermal cycling, microvalves for fluidic manipulation, a co-injector for sizing standard injection, and a 7-cm-long separation channel for capillary electrophoretic analysis. A 9-plex autosomal STR typing system consisting of amelogenin and eight combined DNA index system (CODIS) core STR loci has been constructed and optimized for this real-time human identification study. Reproducible STR profiles of control DNA samples are obtained in 2 h and 30 min with ≤0.8 bp allele typing accuracy. The minimal amount of DNA required for a complete DNA profile is 100 copies. To critically evaluate the capabilities of our portable microsystem as well as its compatibility with crime scene investigation processes, real-time STR analyses were carried out at a mock crime scene prepared by the Palm Beach County Sheriff's Office (PBSO). Blood stain sample collection, DNA extraction, and STR analyses on the portable microsystem were conducted in the field, and a successful “mock” CODIS hit was generated on the suspect's sample within 6 h. This demonstration of on-site STR analysis establishes the feasibility of real-time DNA typing to identify the contributor of probative biological evidence at a crime scene and for real-time human identification.     

An Interactional Perspective of Intimate Partner Violence: An In-depth Semi-structured Interview of a Representative Sample of Help-seeking Women

This article reports a study of women victimized by intimate partner violence (IPV). We describe three interactional aspects of IPV: (1) responses and conduct before, during, and after IPV episodes, (2) impact of alcohol and drug intoxication, and (3) Predictors of risk for IPV victimization in more than one partnership. A representative sample of 157 help-seeking women, recruited from family counseling offices, the police and shelters, were interviewed about physical, psychological and sexual IPV. The nature and characteristics of the IPV interactions were complex and heterogeneous. There were significant interactional differences between the IPV categories concerning the women’s responses and conduct before, during and after the IPV. The impact of alcohol and drug intoxication was relatively small on the occurrence of IPV. About 75% reported that neither the perpetrator nor the female victim had consumed alcohol or drugs before the index IPV exposure. Only 23% of the women had experienced IPV by previous partners. Women who had been subjected to sexual abuse in their family of origin were at almost 25 times increased risk of IPV victimization in more than one partnership. Childhood exposure to physical IPV between parents increased the risk of IPV victimization in more than one partnership significantly more than if the woman had been subject to childhood physical victimization.     

Negligent homicide caused by exhaust gas escaping from a manipulated chimney

A chimney built and operated according to the instructions is supposed to ensure that the combustion gases coming from the fireplace can escape safely. If the operational reliability is impaired, this presents a risk of acute poisoning. The report deals with a negligently caused carbon monoxide poisoning of a married couple as a consequence of improper installation of a cover of the chimney opening. Various causes of fatal poisoning due to defective exhaust systems are discussed in connection with the presented case report.     

The Influence of the Relationship and Motivation on Inequity Aversion in Dogs

One crucial element for the evolution of cooperation may be the sensitivity to others?? efforts and pay-offs in comparison with one??s own costs and gains. If an individual responds to a disadvantageous reward distribution, then it would likely increase its relative fitness compared with those who do not (Brosnan & de Waal, Nature 428:140, 2004). Recent experimental research indicates that sensitivity toward unequal reward distribution is not a uniquely human trait; non-human primates react to inequity if they witness a conspecific that obtains a more attractive reward for the same effort. However, primates are not the only species that cooperate and thus would benefit from inequity aversion. Canids also frequently cooperate with group members (e.g., wolves, wild dogs, etc.) or humans (domestic dogs), allowing for the possibility that they also are sensitive to the reward distribution. We report the findings of two studies of dogs?? responses to inequity and the social factors that mediate such responses. In the first study, we investigated whether domestic dogs showed a response to the inequity of rewards received for the same action in pairs of familiar dogs. We found that dogs showed significant behavioral differences when tested without food rewards when in the presence of a rewarded partner compared to the baseline and asocial control situations. This indicated that the presence of a rewarded partner matters (Range, Horn, Virányi, & Huber, Proc Natl Acad Sci USA 106(1):340?C345, 2009). In contrast to primates, dogs did not react to differences in the quality of food or effort. In the second study, we analyzed whether individual (motivational or personality) characteristics determined the response of each subject to unequal reward distributions or whether the subject??s responses depended on the specific relationship they had with its partner. We found that individual motivation and the quality of the relationship influenced the dogs?? performance in test conditions, but that these factors varied across the control and reward inequity conditions. Overall, our results suggest that inequity aversion in dogs is conditional on their and their partner??s rewards. Whether inequity aversion is based on the same mechanisms in both humans and non-human primates is still unclear.     

Literatur

Ohne Zusammenfassung     

The EDNAP mitochondrial DNA population database (EMPOP) collaborative exercises: organisation, results and perspectives

This paper presents an overview of the organisation and the results of the collaborative exercises (CE) of the European DNA Profiling (EDNAP) Group's mitochondrial DNA population database project (EMPOP). The aim of the collaborative exercises was to determine whether uniformity of mtDNA sequencing results could be achieved among different laboratories. These were asked to sequence either the complete mtDNA control region or the two hypervariable regions HVI (16024-16365) and HVII (73-340) from DNA extracts, buccal swabs or bloodstains, proceeding in accordance with the protocol and strategies used in each individual laboratory. The results of the collaborative exercises were employed to identify possible sources of errors that could arise during the analysis and interpretation of mtDNA profiles. These findings were taken as a basis to tentatively make suitable arrangements for the construction of a high quality mtDNA database. One hundred fifty mtDNA profiles were submitted to the evaluating laboratory, and disaccording profiles were classified into four groups corresponding to the source of error: clerical errors, sample mix-ups, contaminations and discrepancies with respect to the mtDNA nomenclature. Overall, 14 disaccording haplotypes (16 individual errors) were observed. The errors included 10 clerical errors, 3 interpretation problems, 2 cases of sample mix-up and 1 case of point heteroplasmic mixture, where the 2 sequencing reactions brought inconsistent base calls. This corresponds to an error rate of 10.7% in a virtual mtDNA database consisting of the collaborative exercise results. However, this estimate is still conservative compared to conclusions drawn by authors of meanwhile numerous publications critically reviewing published mtDNA population databases. Our results and earlier published concerns strongly emphasize the need for appropriate safety regulations when mtDNA profiles are compiled for database purposes in order to accomplish the high standard required for mtDNA databases that are used in the forensic context.     

Alternative Ulster? Political Parties and the Non-constitutional Policy Space in Northern Ireland

This article uses data from a survey of the candidates in the 2003 Northern Ireland Assembly election to measure the policy positions of the Northern Irish political parties on scales that are the usual measurements of party policy in Western Europe, such as on economic and social issues, the European Union, morality issues, environmental issues and minority groups. These data were then used to test whether the Northern Ireland party system was conducive to integration or consociationalism, which is the current debate on conflict resolution in Northern Ireland. It argues that this debate has been under-theorised and that empirical data can be used to test the likelihood of either scenario. It concludes that, in most aspects, the Northern Ireland party system tends towards the consociational scenario but there are, nevertheless, aspects which suggest that an integrationist scenario could be produced in the long term.     

Institutional Actors in the Brazilian Amazon: Possibilities for NGO and Public Sector Collaboration in Rural Agriculture

Public sector institutions in Amazonian Brazil are experiencing structural reforms, budget cuts, and overburdened missions. Services needed to support appropriate resource use practices by rural farmers are gradually being provided by NGOs where the public sector has failed to provide them. But local NGOs are institutionally weak, and their activities are poorly coordinated with other regional service providers. This article analyzes three models for NGO organization and partnership that were designed outside of the Amazon region, and tests their applicability to the small-farming sector in Pará State in the eastern Brazilian Amazon. With some adaptation, these models can effectively help coordinate three overarching needs in the region: institutional strengthening, regional planning, and improving program effectiveness and efficiency.     

Co-occurrence and Correlates of Posttraumatic Stress Disorder and Major Depression in Physically Abused Women

Ninety two women presenting for treatment for marital problems and who were physically victimized by their spouses (e.g., pushing, shoving, punching) within the past year participated in this study. There were three study objectives: (1) document rates and co-occurrence of posttraumatic stress disorder (PTSD) and Major Depressive Disorder (MDD), (2) identify predictors of PTSD and depression symptom frequency/severity, and (3) systematically evaluate disorder-specific group differences in marital- and marital violence-related factors. Within the month prior to assessment, 29.8% of the sample met diagnostic criteria for PTSD and 32% for MDD. MDD and PTSD were significantly, but moderately, correlated at both the symptom and diagnosis levels. However, PTSD symptom frequency and depression symptom severity were predicted by different marital- and marital violence-related factors. PTSD symptoms were predicted by spouse's dominance/isolation tactics and intensity of husband-to-wife physical aggression while depressive symptoms were predicted by marital discord and intensity of husband-to-wife physical aggression. Comorbid women and those with PTSD only reported significantly more spousal fear and husband-to-wife physical aggression than those with MDD only or neither disorder. No group differences were found on rate of marital discord or spouse's controlling/isolating tactics. Results are discussed in terms of theoretical and treatment implications for abused women seeking treatment for marital conflict.