Characterisation of forward stutter in the AmpFlSTR® SGM Plus® PCR

PCR amplification of tetrameric short tandem repeats (STRs) can lead to Taq enzyme slippage and artefact products typically one repeat unit less in size than the parent STR. These back stutter or n ? 4 amplification products are low-level relative to the amplification of the parent STR but are widely seen in the forensic community where tetrameric STRs are employed in the generation of DNA profiles. To aid the interpretation of DNA mixtures where minor contributor(s) might be present in comparable amounts to the back stutter products, the typical amounts of back stutter generated have been well characterised and guidelines for interpretation are in place. However, further artefacts thought to be Taq enzyme slippage leading to products with one repeat unit greater than the parent sequence (n + 4 or forward stutter) or two repeats less (n ? 8 or double back stutter) also occur, but these have not been well characterised despite their potential influence in mixture interpretations. Here we present findings with respect to these additional artefacts from a study of 10,000 alleles and include guidelines for interpretation.     

Vladimir Putin and the Reaffirmation of Central State Power

Using post-Soviet Kazakhstan as a conceptual point of departure, this article considers the role that proactive framing and persuasion play in ensuring regime survival in soft authoritarian contexts. Drawing on interviews, opinion polls, news media, and the scholarly literature, the authors use three examples—Kazakhstan's OSCE bid, the global financial crisis, and "Rakhatgate"—that highlight the regime's varying proportions of persuasive and coercive efforts. The ways a soft authoritarian leader responds to potentially threatening events are examined. Non-material sources of regime durability are analyzed as essentials for understanding authoritarian regime dynamics and, by implication, for developing a full theory of regime change.     

Efficient,Adaptable Simulations: A Case Study of a Climate Negotiation Game

Instructors may be reluctant to adopt simulations because of time, labor, or material constraints, or perceived incompatibility with large classes. In fact, simple games can cover multiple key concepts with minimal time and effort by the instructor. Simple games are also adaptable to other topics and classes, including large lectures. This article presents a simulation in which students negotiate a global greenhouse gas reduction agreement. Three scenarios model basic climate change mitigation, follow-on agreements for climate stabilization, and the surprise withdrawal of signatories after a domestic leadership turnover (e.g., the 2016 U.S. presidential election). The simulation teaches key concepts such as anarchy, collective action, preference divergence, and commitment problems. Concepts such as institutions, identity, and levels of analysis arise organically from game play. The exercise has extremely low cost and setup time. It can be run in 15 minutes or extended for a full class period. The game may also be repurposed to simulate other bargaining or collective action issues. This case study shows that simulations can be efficient and adaptable. Instructors can create their own simple games to enhance comprehension of key concepts.     

Domestic Violence and the Gendered Law of Self-Defence in France: The Case of Jacqueline Sauvage

Legal responses to battered women who kill have long animated scholarly debate and law reform activity. In September 2012 after 47 years of alleged abuse, Frenchwoman Jacqueline Sauvage fatally shot her abusive husband three times in the back. The subsequent contested trial, conviction for murder, unsuccessful appeal and later presidential pardon of Sauvage thrust the French law of self-defence into the spotlight. The Sauvage case raises important questions surrounding the adequacy of the French criminal law in this area, the ongoing proliferation of gendered stereotypes in law and the need for reform. In the wake of the Sauvage case, this article provides a timely analysis of the gendered law of self-defence in France. Drawing from an in-depth analysis of the judgments imposed in the Sauvage case, this article examines the adequacy of French legal responses to battered women who kill and ignites an argument for further law reform.     

Policies for Decent Labour Standards in Britain

Decent labour standards are a prerequisite for perceived justice and social cohesion. Insofar as they have been achieved in Britain in the past, it has been the result of collective bargaining between employers and trade unions. This has all but vanished in the private sector and, it is argued, there is no chance of its being revived. Upholding labour standards now lies in the provision of statutory individual employment rights. Experience with minimum wages provides some guidance on how these might be developed through social partnership arrangements. Once achieved, such rights amount to little without effective enforcement. Increasingly important for this is the use of the law and consumer campaigns to expose poor employment practices and complex supply chains so that offending employers can be held to account. If Britain is to avoid falling into a competitive ‘race to the bottom’ with Brexit, it must institute a robust means of implementing and enforcing decent labour standards.     

Are There Hidden Costs Associated With Conducting Layoffs? The Impact of Reduction‐in‐Force and Layoff Notices on Teacher Effectiveness

Few studies examine employee responses to layoff‐induced unemployment risk; none that we know of quantify the impact of job insecurity on individual employee productivity. Using data from the Los Angeles Unified School District (LAUSD) and Washington State during the Great Recession, we provide the first evidence about the impact of the layoff process on teacher productivity. In both sites we find that teachers impacted by the layoff process are less productive than those who do not face layoff‐induced job threat. LAUSD teachers who are laid off and then rehired to return to the district are less productive in the two years following the layoff. Washington teachers who are given a reduction‐in‐force (RIF) notice and are then not laid off have reduced effectiveness in the year of the RIF. We argue that these results are likely driven by impacts of the layoff process on teachers’ job commitment and present evidence to rule out alternate explanations.     

“In the Best Interests of the Child”: Mapping the (Re) Emergence of Pro‐Adoption Politics in Contemporary Australia

This article seeks to understand, in historical and international perspective, recent governmental initiatives that aim to reinstate adoption as a viable policy option for the care and placement of children in Australia, with reference to two recent reports of the House of Representatives Standing Committee on Human and Family Services, Overseas Adoption in Australia: Report of the Inquiry into Adoption of Children from Overseas (2005), and The Winnable War on Drugs: The Impact of Illicit Drug Use on Families (2007) which raises adoption as a policy option for children of drug‐addicted parents. These reports appear to signal a discursive shift away from the anti‐adoption attitudes that have characterised the post‐1970s period in response to the Stolen Generations and other past adoption practices. It is argued that this change can be understood as having been pushed to the fore by the conservative family policy of the Howard era and further fostered by international trends in adoption policy.     

Adolescents’ Identity Formation: Linking the Narrative and the Dual-Cycle Approach

The narrative and dual-cycle approach conceptualize and operationalize adolescents’ identity formation in different ways. While the narrative approach focuses on the construction of an autobiographical life story, the dual-cycle approach focuses on the formation of identity commitments. Although these approaches have different emphases, they are conceptually complementary. Yet, their empirical links and distinctions have only scarcely been investigated. Empirical knowledge on these links in adolescence and across time has been especially lacking. In the present research, it was therefore examined whether key characteristics of adolescents’ narration (autobiographical reasoning and agency) were concurrently and prospectively related to engagement in the dual-cycle processes of commitment making, identification with commitment, exploration in breadth, exploration in depth, and ruminative exploration. The findings from a cross-sectional sample of 1,580 Dutch adolescents (M_age?=?14.7 years, 56% female) demonstrated that autobiographical reasoning was significantly positively associated with the commitment and more adaptive exploration processes (i.e., in breadth and in depth). In addition, agency was significantly positively associated with the commitment processes and exploration in depth. Yet, these associations between the narrative characteristics and dual-cycle processes were only weak. Subsequently, the findings from a two-year longitudinal subsample (n?=?242, M_age?=?14.7 years, 62% female) indicated that on average commitment strength remained stable but exploration increased across middle adolescence. A stronger increase in identification with commitment and adaptive exploration (i.e., in breadth and in depth) was predicted by a higher degree of agency in adolescents’ narratives. Overall, these findings indicate that both approaches to identity formation are associated, but the small size of these associations suggests that they predominantly capture unique aspects of identity formation. Both approaches could thus complement and inform each other.

     

Elevator-Related Deaths

Elevators are mechanical transportation devices used to move vertically between different levels of a building. When first developed, elevators lacked the safety features. When safety mechanisms were developed, elevators became a common feature of multistory buildings. Despite their well-regarded safety record, elevators are not without the potential for danger of injury or death. Persons at-risk for elevator-related death include maintenance and construction workers, other employees, and those who are prone to risky behavior. Deaths may be related to asphyxia, blunt force, avulsion injuries, and various forms of environmental trauma. In this review, we report on 48 elevator-related deaths that occurred in nine different medicolegal death investigation jurisdictions within the United States over an approximately 30-year period. The data represents a cross-section of the different types of elevator-related deaths that may be encountered. The review also presents an overview of preventive strategies for the purpose of avoiding future elevator-related fatalities.     

Navigating tensions in co‐production: A missing link in leadership for public value

New forms of public leadership are needed to design and implement co‐production of public value. We draw on narrative analysis to explore the barriers to developing the capacity for co‐production of public value in disadvantaged and vulnerable Indigenous communities and find that existing norms not only crowd out efforts at reform, but can work against them. In particular, we find that upward accountability and a focus on expert knowledge are the most problematic for co‐production of public value. We focus on how more traditional practices of public administration mediated the achievements of public leaders in their co‐production efforts when Australian governments introduced strategies for partnership with Indigenous communities for services design and delivery. The analysis suggests that creating a broader enabling environment of supportive logics would allow public leaders to engage more productively with inherent tensions between old and new ways of working.