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Pulmonary thromboembolism (PE) is found commonly in forensic pathology practice, as it typically causes sudden death. It is attributed to a wide variety of predominantly acquired etiologies. Although likely etiologically multifactorial, some common proximate causes include: surgery, pregnancy, injury, inactivity of any cause, cancer, obesity, or serum hyperviscosity. On occasion, no apparent predisposing condition is identified. In these instances, occult hereditary thrombophilias may play a causal role. Deaths referred to the Office of Chief Medical Examiner (OCME) of New York City between December, 2000 and September, 2003 and due to PE were retrospectively reviewed. Molecular analysis (FRET) was performed on selected cases for three common hereditary thrombophilias: mutations in factor V Leiden (FVL), prothrombin G20210A (PT), and methylenetetrahydrofolate reductase (MTHFR).During the study period, 124 of 15,280 deaths were primarily attributable to PE. Of those, 34 were selected for molecular analysis. One or more mutations were detected in 35% of those, five of which were clearly causally related to death. Given the potential benefits to surviving family members, our data indicate that postmortem molecular testing for the common hereditary thrombophilias is warranted in at least selected cases. 相似文献
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This paper examines the potential of infrared chemical (hyperspectral) imaging as a technique for the forensic analysis of automotive paint chips in particular, and multicomponent (e.g., layered) samples in general. Improved sample preparation procedures for the infrared analysis of paint chips are detailed, with the recommendation that where mounting resins are chemically incompatible with the sample, it is better to mount and section the sample in a soft wax from which the sections can be removed and pressed into a KBr disk for transmission analysis. Infrared chemical images of multilayered paint chips have been successfully obtained, with the chief advantage over conventional infrared analysis being that thousands of infrared spectra are collected in a few minutes across the whole sample, at a spatial resolution of around 5 microm. As with conventional infrared spectroscopy, chemical species can be identified from their spectra, but the wealth of information available can be also extracted in a number of different ways that make multicomponent spectral (and hence chemical) comparisons between two samples easy to visualize and understand. In one approach, the infrared chemical images of two paint chips being compared side-by-side can be viewed as a "movie," in which each frame is an intensity map of the two samples at a given wavenumber (frequency) value. In another approach, the spectra (pixels) in the image files are classified into chemically similar groups, resulting in a "cluster" image that makes it possible to simultaneously compare all of the layers in two paint chips. These methods are applicable to other multicomponent samples, and also to other chemical imaging techniques. 相似文献
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The purpose of this study was to compare the effectiveness of the QIAGEN QIAamp Stool Mini Kit against a standard phenolchloroform procedure for the extraction, quantitation, and STR-typing of human nuclear DNA from human feces. Stools from six subjects were sampled by swabbing and excision. Samples extracted with the QIAamp kit gave a wide range of DNA yields, whereas those extracted by the organic method yielded no DNA. DNA was not recovered from one subject's stools by either procedure. The QIAamp extracts were amplified with the Profiler Plus and COfiler kits, and PCR inhibition was observed with DNA extracts that were further concentrated. Substitution of water or TE-4 for the QIAamp elution buffer eliminated most, if not all, of the inhibition. A modified QIAamp procedure was used to extract thirty samples, which were subjected to one of five environmental conditions. DNA was recovered from all of these samples, and typing results were obtained on 93% of the samples. 相似文献
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Janis?E.?Jacobs Christina?S.?ChhinEmail author Katherine?Shaver 《Journal of youth and adolescence》2005,34(2):61-72
This study examined: (1) relations between parents prior stereotypes about adolescence and their later beliefs about their own child during early and middle adolescence, (2) relations between parents stereotypes and their own childs behaviors during middle and late adolescence, and (3) the role of congruency between mothers and fathers beliefs about adolescence in predicting their childs behavior. The results revealed significant relations between parents prior stereotyped beliefs and their specific beliefs about their own children during the 7th and 10th grades. In addition, the relations between parents prior stereotypes and their adolescent childrens behaviors measured at 3 and 5 years later (10th and 12th grades) were estimated. Parents stereotypes about adolescence significantly predicted their childrens behaviors in both 10th and 12th grades. Finally, the congruency between mothers and fathers stereotyped beliefs was significantly related to their childrens behaviors in the 10th and 12th grades. Parents who had consistent and strong stereotypes about adolescence had adolescent children with more deviant peers than parents with consistent, but less-stereotyped views.Portions of this paper were presented at the 2002 biennial meeting of the Society for Research in Adolescence, New Orleans, LA. This research was made possible by grants from NSF, the Spencer Foundation, and the William T. Grant Foundation to Jacquelynne Eccles and Bonnie Barber.Professor of Psychology, and Human Development and Family Studies, Pennsylvania State University. Received PhD in Psychology from the University of Michigan. Current interests include the development of social cognitive processes during childhood and adolescence, and gender differences in achievement motivation, self-perceptions of achievement, and parents influences on achievement.Doctoral Candidate and Research Assistant, Human Development and Family Studies, Pennsylvania State University. Received MS in Human Development and Family Studies from the Pennsylvania State University. Current interests include gender socialization and activity involvement during adolescence.Research Associate, North Carolina Center for Nursing. Received MS in Human Development and Family Studies from the Pennsylvania State University. Current interests include adolescent development. 相似文献
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Nina S. Mounts David P. Valentiner Katherine L. Anderson Michelle K. Boswell 《Journal of youth and adolescence》2006,35(1):68-77
A path model was tested in an ethnically diverse sample of 350 college students in which shyness, sociability, and parental
support for the college transition were related to loneliness and friendship quality. Furthermore, friendship quality and
loneliness were related to depression and anxiety. High levels of shyness, low levels of sociability, and low levels of parental
support were related to high levels of loneliness. High levels of parental support for the college transition were related
to more positive friendship quality. Multiple regression analyses suggested that loneliness, but not friendship quality, were
related to adolescents’ anxiety and depression. In addition, the interaction between shyness and sociability was significantly
related to anxiety for African-American adolescents such that adolescents who reported low levels of sociability in combination
with high levels of shyness reported the highest levels of anxiety. There was also a significant interaction between sociability
and parental support for African-American adolescents such that high levels of sociability in combination with low levels
of parental support for the college transition were related to high levels of anxiety. For White adolescents, only loneliness
was related to anxiety.
Nina S. Mounts is an associate professor at Northern Illinois University. She received her PhD in child and family studies
from University of Wisconsin-Madison. Her major research interests are on linkages between parent and peer contexts, parental
management of adolescents’ peer relationships, and peer influence on adolescents.
David P. Valentiner is an associate professor at Northern Illinois University. He received his PhD in clinical psychology
from University of Texas-Austin. His major research interests are cognitive and emotional factors in anxiety disorders, including
the development and maintenance of social anxiety.
Katherine Anderson is a graduate student at University of Wisconsin-Madison. She received her MS in developmental psychology
from Northern Illinois University. Her major research interests are on career identity development in college students.
Michelle Boswell is a graduate student at Northern Illinois University. She received her MS in clinical psychology from Northern
Illinois University. Her major research interests are on parenting effects on bullying and victimization. 相似文献