Polymorphism of four X-chromosomal STR loci in Belarusians and Slovaks

We have investigated four X-chromosomal STR loci (a tetranucleotide repeat marker HPRTB, DXS7423 and trinucleotide repeat systems DXS101, DXS8377) in population samples of unrelated Belarusian and Slovak males (N = 180 and 116, respectively).Markers were amplified in a multiplex PCR reaction with primers labeled with fluorescent dyes 5-FAM and 5-JOE (dye set F). The separation and detection of PCR products were performed by capillary electrophoresis on a 3130 Genetic Analyzer (Applied Biosystems), using positive controls (K562 and NA9947A) and the GeneScan-500 ROX internal lane standard. Allele designation was based on comparison with the constructed allelic ladder. We performed statistical analysis estimating power of discrimination for males (PD_male). Our data was compared with European populations from Poland and Germany. The obtained data are useful in forensic practice and they contribute to creation of national X-STR databases in Belarus and Slovakia.     

Lebensgef?hrte der Mieterin im Schutzbereich des Mietvertrags?

Die Schutz- und Sorgfaltspflichten des Bestandgebers (hier die Pflicht zur R?umung und Streuung des Besucherparkplatzes) bestehen nicht nur gegenüber dem Bestandnehmer, sondern auch gegenüber den zu dessen Hausgemeinschaft geh?renden Familienangeh?rigen und Hausangestellten, nicht jedoch gegenüber Personen, die sich nur kurzfristig in den Mietr?umen aufhalten, wie G?ste, Lieferanten, Handwerker oder blo?e Besucher, mag es sich dabei auch um Angeh?rige des Mieters handeln. Der Lebensgef?hrte der Mieterin geh?rt nur dann zum Schutzbereich des Mietvertrags, wenn er mit ihr in Wohnungsgemeinschaft lebt und somit zu ihrer Hausgemeinschaft geh?rt.     

Acute cardiovascular fatalities following cannabis use.

We report six cases of possible acute cardiovascular death in young adults, where very recent cannabis ingestion was documented by the presence of tetrahydrocannabinol (THC) in postmortem blood samples. A broad toxicological blood analysis could not reveal other drugs. Similar cases have been reported in the literature, but the toxicological analysis has been absent or limited to urine samples, which represent a much broader time window for cannabis intake. This paper presents six case reports, where cannabis alone was detected in blood. Further, an overview over previously published cases, clinical trials and possible patho-physiological mechanisms are presented.