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11.
We analyze locational policy coordination in the metropolitan regions of secondary capital cities. Secondary capital cities—defined as capitals that are not the primary economic city of their nation states—serve as the political center of their nation states; however, they must simultaneously explore new ways to develop their own regional economies. Locational policies, and their regional coordination, aim to strengthen the economic competitiveness of metropolitan regions. Our comparison of the metropolitan regions of Bern, Ottawa–Gatineau, The Hague, and Washington, D.C., reveals that vertical institutional fragmentation, together with high local tax autonomy, create an unlevel playing field, which prompts jurisdictions to behave fiercely in regional tax competition. These findings are troubling for secondary capital cities given their propensity to be located in fragmented metropolitan regions and the capital city‐specific local tax autonomy constraints imposed on them.  相似文献   
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Firms across the globe are affected by red tape, but there is little academic research on how country‐level institutions shape red tape perceptions. Drawing on institutional theory, we argue that a variety of formal and informal country‐level institutions affect perceptions of red tape in the private sector. We test our hypotheses using six data sources, including the World Economic Forum and the World Bank. Our results indicate that red tape is weakly associated with a country's level of formalization and rule enforcement effectiveness and more prevalent in federal as opposed to unitary states. As for informal institutions, we find that red tape perceptions are more pronounced in countries with an increased conservative political ideology, higher levels of corruption, and cultures that emphasize individualism and uncertainty avoidance. We conclude with a discussion of the implications for theory and practice.  相似文献   
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This study examines the attributes of organizational rules that influence rule following. Rule following fosters organizational effectiveness by aligning individual behaviours with organizational preference. While a range of theoretical explanations have been offered for rule following, the characteristics of rule design and implementation have received less empirical attention. Borrowing from the green tape theory of effective rules, this study examines the influence of two particular characteristics—rule formalization and rule consistency—on rule following. Three studies, which include two vignette experiments and a survey of two local government organizations, provide the data for the research. The results suggest that rule formalization and rule consistency independently increase rule following, with mixed evidence of interaction effects. The broad implication is that public managers must attend to both rule design and implementation to foster organizational rule following.  相似文献   
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In order to evaluate histological features of freezing damages to human tissue after death, we froze samples of liver and heart tissue to temperatures of -12 degrees C, -28 degrees C and -80 degrees C, and stored them for 24 and 72 h, respectively, at those temperatures. After thawing and routine preparation for histology, the samples were evaluated both by microscope and with an electronic image analyzer. In all cases, we found extended extracellular spaces and shrunken cells resulting from the freeze-thaw cycle. These features were more pronounced in tissues stored for longer durations. Such findings seem to be typical of tissue that has been frozen prior to examination. Two cases of dead bodies found outdoors at subzero temperatures demonstrate that formerly frozen and unfrozen tissues can be distinguished histologically. The findings are examined in relation to the fundamental laws of cryobiology.  相似文献   
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A sensitive and specific method for the determination of gamma-hydroxybutyric acid (GHB) in urine and serum is described. Prior to quantification by LC/MS in a Pauli-type ion trap, the molecule is converted by a fast and simple one-step procedure into its n-butyl ester derivative. Hexa-deutero GHB has been used as internal standard.  相似文献   
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Party organisations are often 'black boxes' overlooked by scholars in favour of party leaders, manifestos and elections. Yet intra-party dynamics are often crucial. This article uses the example of the Ulster Unionist Party (UUP) to illustrate this. Events in the 1998–2003 period highlight the importance of splits within the party's governing Ulster Unionist Council (UUC) over the Good Friday Agreement (GFA) which underpins the Northern Ireland peace process. This article pioneers the use of postcode-profiling technology and uses multi-level analysis of a UUC party list to show that delegates' party section provides the best predictor of their stance towards the Good Friday Agreement. Consequently, the article argues that delegate voting behaviour within the UUC is best explained by reference to highly embedded, meso-level institutions which perpetuate networks and condition individual preferences.  相似文献   
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DNA extracted from 33 postmortem muscle specimens was analyzed using MZ 1.3, a hypervariable minisatellite probe, as well as locus-specific minisatellite probes (g3, MS1 and MS43). After storage at -25 degrees C for 10 months, DNA from all the samples was partially (approximately 21% of total DNA) degraded even when autopsy was performed 1 day postmortem. However, more than 90% of DNA samples up to at least 3 days postmortem were suitable to obtain good restriction fragment length polymorphism (RFLP) patterns. When small strips of specimen were stored for 8 days at room temperature in moist chambers, approximately 42% of total DNA was degraded. Only 30% of these DNA samples still showed good RFLP patterns. However, no obvious relation between qualities of DNA analyzed by detection of RFLP and quantities of total and high-MW DNA became apparent. A case of familial relationship was ascertained by DNA fingerprints. Since DNA of good quality can be recovered from muscle tissues in large quantities, DNA extraction from muscle tissues and detection of RFLP patterns should be very useful for individual identification in autopsy cases.  相似文献   
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