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121.
比较D1S80 ,DQA1+PM和CTT等位点在法医物证鉴定中应用情况 ,经研究发现 ,D1S80位点识别能力较强 ,基因遗传稳定 ,其电泳检测操作简单 ,易推广 ;DQA1+PM位点扩增片段较短 ,反向斑点杂交法灵敏度高 ,特别适用于陈旧、降解和微量生物检材的鉴定 ;CTT位点的荧光检测法稳定、可靠 ,实验结果便于保存和管理。总之 ,D1S80、DQA1+PM和CTT位点的遗传符合孟德尔定律 ,分型简便、可靠 ,在亲子鉴定和个人识别中发挥重要作用。 相似文献
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Zúñiga J Ilzarbe M Acunha-Alonzo V Rosetti F Herbert Z Romero V Almeciga I Clavijo O Stern JN Granados J Fridkis-Hareli M Morrison P Azocar J Yunis EJ 《Forensic science international》2006,164(2-3):266-270
Allelic frequencies of 15 short tandem repeats (STR) markers (CSF1PO, FGA, THO1, TPOX, VWA, D3S11358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, D19S433 and D2S1338) were determined using the AmpFl STR Identifiler PCR Amplification Kit in Puerto Rican American individuals (N=205) from Massachusetts. The FGA, D18S51 and D2S1338 loci had a high power of discrimination (PD) with values of 0.967, 0.965 and 0.961, respectively. Significant deviations from the Hardy-Weinberg (HW) equilibrium were not detected. An important genetic contribution of Caucasian European (76.4%) was detected in Puerto Rican Americans. However, comparative analysis between Puerto Rican American and other neighboring populations from United States mainly with African and Caucasian Americans, revealed significant differences in the distribution of STR markers. Our results are important for future comparative genetic studies of different American ethnic groups, in particular a cultural group called Hispanic-Americans and should be helpful for forensic and paternity testing. 相似文献
125.
A new method of age estimation based on the changes in human non-collagenous proteins from dentin 总被引:1,自引:0,他引:1
The new method exploits the characteristic of staphylococcal protease V8, which specifically splits peptide bonds where l-glutamic or l-aspartic acid participate. Those peptide bonds where d-aspartic acid is present remain unsplit because of the stereospecifity of enzymes. In accordance with expectations, fewer peptide bonds are split by this protease at more advanced ages and larger peptide fragments are thus formed due to the higher content of d-amino acid residues in the proteins of older people. The samples of acid-extracted non-collagenous proteins from dentin were separated using high performance liquid chromatography after enzymatic hydrolysis. A peak with a retention time of 45.3 min was chosen and his enlarging area showed a linear correlation with increasing age. Although the linear correlation with age was proved, the scattering of values decreases the usefulness of the proposed method for age estimation. 相似文献
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Bligh-Glover WZ Miller FP Balraj EK 《The American journal of forensic medicine and pathology》2004,25(3):255-258
Two cases of suicidal electrocution are presented: one using a household lamp, the other using a homemade electrocution machine. The mechanisms of death and the histologic changes in electrocution are discussed. 相似文献
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An unconscious 8 weeks old infant was admitted to hospital and found to have bilateral, subdural and retinal haemorrhages. He died the following day. The explanation for the subdural haemorrhage put forward by his carers was that the infant had been in a baby-rocker and that the carers had seen the rocker being rocked vigorously by their 14 months old daughter on two separate occasions. This paper describes the biomechanics of an infant model in the particular baby-rocker used and determines the maximum forces generated, comparing them with the 'forces' thought to be compatible with the causation of subdural haemorrhage. 相似文献
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