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71.
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Development of a Real‐Time PCR‐Based Method for Analyzing Semen‐Specific Unmethylated DNA Regions and Methylation Status in Aged Body Fluid Stains
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Ken Watanabe Ph.D. Tomoko Akutsu Ph.D. Koichi Sakurada D.D.S. Ph.D. 《Journal of forensic sciences》2016,61(Z1):S208-S212
The detection of semen in forensic investigation is considered important evidence of sexual assault. In this study, we report the development of a real‐time polymerase chain reaction‐based method for identifying semen that can simply and rapidly analyze the semen‐specific unmethylated region of the DACT1 gene. Using two fluorescent probes designed for the methylated or unmethylated status, this method could perform quantitative analysis of the methylation status in this region. Furthermore, this method was used to analyze various body fluid samples, including 29‐year‐old semen and blood stains. The results showed that this method can detect almost exclusively semen or nonsemen signals even in highly decomposed samples, while a few semen or nonsemen samples showed slight signals of the other fluorescence probe. Although there is still a need for further analysis such as setting thresholds to analyze unknown samples, this method could be a useful supplementary tool for identifying semen, especially in old stains such as those in cold‐case investigations. 相似文献
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Ken Shimizu 《Asia-Pacific Review》2018,25(1):112-123
The year 2018 marks the 150th anniversary of the establishment of diplomatic relations between Japan and Sweden. It is believed that the first contact between Sweden and Japan came during the Edo Period and was mediated by the Netherlands. The Treaty of Friendship, Commerce and Navigation between Japan and Sweden, signed in 1868, also came into being thanks to the good offices of the Dutch. This treaty, like many others into which Japan entered at that time, was an “unequal treaty.” As Japan increased its presence in East Asia, this unequal treaty with Sweden was revised in 1911. The world was changing. Sweden and Japan shared a common interest in opposing Russia’s ambitions. Russia in particular was a common threat to the diplomatic posture and security of both Japan and Sweden. These two nations, positioned like bookends on either side of Russia, could be important strategic partners for one another and allies in their relationships with Russia. Considering the current issue of North Korea, Sweden also has the potential to act as a mediator because it maintains diplomatic relations with North Korea. There is reason to believe that the diplomatic relationship between Japan and Sweden will develop further. 相似文献
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A. M. Levi 《心理学、犯罪与法律》2013,19(3):273-285
Abstract Simultaneous lineups allow witnesses to compare lineup members, causing excessive mistaken identifications. Levi (1998b) has tested MSL lineups: they are sequential, larger, and allow multiple choices. [The MSL lineup was originally termed a Modified Sequential Lineup (Levi, 1998b). However, there are other modified sequential lineups.] Each factor decreases mistaken identifications. However, witnesses make fewer single choices of culprits. Sometimes witnesses choose suspects more confidently than any foil. This analysis examines such multiple choices in four experiments. They account for half of multiple choices with culprits. Few foils are chosen, and such responses are rare in culprit-absent lineups, no more than single choices. They are therefore identifications too. An experiment comparing simultaneous, sequential, and MSL lineups is also reported. The culprit was identified more in simultaneous lineups than in sequential ones. The simultaneous lineup had more mistaken choices than sequential and MSL lineups, whose results were identical. The simultaneous and sequential lineups were equally diagnostic, while the MSL lineup, four times larger, was more than four times more reliable. 相似文献
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Ken Young 《Intelligence & National Security》2013,28(6):901-925
When Socialist intellectual John Strachey was appointed as Secretary of State for War in 1950, his pre-war record as a Marxist writer with close connections to the Communist Party of Great Britain (CPGB) became a matter of public debate. A bitter campaign was run against him in the Beaverbrook press, and some members of the US defense and nuclear establishment pressed for an embargo on sensitive information being passed to the UK War Office. American suspicion of the political reliability of the Labour government was heightened by the appointment, but this does not explain how and why some Americans were so hostile to Strachey. The FBI's dossier on his pre-war activities, circulated amongst his American critics, documented Strachey's supposed secret membership of the CPGB's Central Committee. MI5 and Special Branch files show that this supposition was based on faulty intelligence. The readiness of American anti-Soviet protagonists to lend credence to such suspicions contrasts with the relaxed view of Strachey's past that was taken in Whitehall. Both positions were characteristic of their time, and of this stage in the Anglo-American alliance. This paper explores the ways in which American insecurities and a British climate of tolerance towards fellow travellers shaped the way that episode played out. 相似文献
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Agricultural intensification, or increasing yield, has been a persistent theme in policy interventions in African smallholder agriculture. This article focuses on two hegemonic policy models of such intensification: (1) the ‘Alvord model’ of plough-based, integrated crop-livestock farming promoted in colonial Zimbabwe; and (2) minimum-tillage mulch-based, Conservation Agriculture, as currently preached by a wide range of international agricultural research and development agencies. An analysis of smallholder farming practices in Zimbabwe's Zambezi Valley, reveals the limited inherent understanding of farmer practices in these models. It shows why many smallholder farmers in southern Africa are predisposed towards extensification rather than intensification, and suggests that widespread Conservation Agriculture adoption is unlikely. 相似文献
79.
David Levi‐Faur 《Law & policy》2013,35(1-2):29-50
This article assesses the “odyssey” of the regulatory state from a mere American thin and monomorphic concept to a global, thick and polymorphic concept that captures some of the more important features of the capitalist–democratic state. The burgeoning literature on the regulatory state presents a confusing number of images and characterizations that are increasingly conflicting, and it too often presents a monomorphic conception of the regulatory state. The article suggests that we need to define the regulatory state rather than merely characterizing it. And we need to do so in a manner that will allow us to move beyond the specific institutional features of a certain era, nation, region, or sector. Rather than contrasting regulation with distribution and redistribution, and contrasting the regulatory state with other forms of state, I treat the regulatory state as one morph of the polymorphic capitalist state, a morph that may help constitute other morphs (such as the welfare state and the developmental state) instead of replacing them. This in turn may help remove the artificial walls between the regulatory scholarly community and other social scientists, and promote more fruitful social science. 相似文献
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