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DNA quantification of forensic evidence is very valuable for an optimal use of the available biological material. Moreover, sex determination is of great importance as additional information in criminal investigations as well as in identification of missing persons, no suspect cases, and ancient DNA studies. While routine forensic DNA analysis based on short tandem repeat markers includes a marker for sex determination, analysis of samples containing scarce amounts of DNA is often based on mitochondrial DNA, and sex determination is not performed. In order to allow quantification and simultaneous sex determination on minute amounts of DNA, an assay based on real-time PCR analysis of a marker within the human amelogenin gene has been developed. The sex determination is based on melting curve analysis, while an externally standardized kinetic analysis allows quantification of the nuclear DNA copy number in the sample. This real-time DNA quantification assay has proven to be highly sensitive, enabling quantification of single DNA copies. Although certain limitations were apparent, the system is a rapid, cost-effective, and flexible assay for analysis of forensic casework samples. 相似文献
123.
A comparison of adjustment methods to test the robustness of an STR DNA database comprised of 24 European populations 总被引:3,自引:0,他引:3
Gill P Foreman L Buckleton JS Triggs CM Allen H 《Forensic science international》2003,131(2-3):184-196
An aim of the European Network of Forensic Science Institutes (ENFSI) is to produce a DNA database of second generation multiplex (SGM) STR profiles that is representative of the resident cosmopolitan populations. To achieve this, data were collected from 24 different populations. All of the data were combined to form one database of 5700 profiles from which allele proportions were calculated. The robustness of this combined European database was tested by estimating parameter d for every DNA profile, where d=log(10)(Pm(c)/Pm(E)) Pm(c) is the match probability of the profile calculated from its cognate database and Pm(E) is the match probability of the combined European database. Overall there was a small tendency for Pm(c)>Pm(E) primarily because of sampling bias. This bias was removed by the simple expediency of applying an adjustment factor to the calculation of Pm(E). These were selected from the Balding size bias correction, the Balding and Nichols Fst correction, a minimum allele proportion (between 0.01 and 0.02), an upper bound of a 95% confidence interval (CI) and a lower bound on the genotype match probability. It was demonstrated that a single European database is a feasible proposition. A combination of different adjustment methods can be used to ensure that the result is conservative relative to the cognate database, and their effect measured by parameter d. 相似文献
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This article continues the themes developed in a previous paper looking at reparations for past wrongs in post-colonial Australia.
It narrows the focus to examine the scope of the law of tort to provide reparations suffered as a result of colonisation and
dispossession, with particular emphasis on the assimilation policies whose legacy is now known emphatically, although it ought
not be exclusively, as the Stolen Generations. The search for more than just words is particularly topical in light of the
Australian Prime Minister’s formal apology in early 2008. This highly symbolic step must not lose momentum. That momentum
could be both political and legal. In the latter context, we explore here the concept of alien tort, in the light of current
Australian tort jurisprudence, which has thus far proved inadequate to achieve compensation for the harms suffered. Taking
a comparative approach, procedural and substantive developments are to better deliver justice consistent with community values.
In particular, the greater acceptance of psychological harm as actionable tortious conduct in Canada and the United States
is examined. The article further examines the scope for stolen children to bring suit under the US Alien Tort Claims Act 1789. While the Australian government enjoys sovereign immunity in the courts of the United States, non-state organisations such
as churches that were complicit in the forcible removals may be subject to United States jurisdiction wherever they have a
presence in that country. The procedural and substantive elements of such a claim are discussed. In particular, the relevant
cause of action, the issue of forum non conveniens and the statute of limitations will be analysed to map out whether a claim could be developed and successfully prosecuted. 相似文献
129.
Veruska Muccione Boris Orlowsky Simon K. Allen Christian Huggel Nadine Salzmann Nilton Montoya 《Development in Practice》2019,29(3):336-348
This article reflects on the merits and shortfalls of bilateral research programmes aimed at strengthening climate change research capabilities, using the experience from two programmes, the PACC and IHCAP in Peru and India, respectively. The study highlights key aspects of these types of bilateral programmes, namely: capacity; performance, salary and appreciation; funding; bureaucracy and hierarchy; publishing; and data sharing. Furthermore, it emerged that these programmes would benefit from a more extensive consolidation phase of the research activities and partnership rather than rapidly transferring into out- and up-scaling phases. 相似文献
130.
Representative democracies require sufficient numbers of citizens to put themselves forward as candidates for political office. Existing studies have shown that political institutions are not representative of the population as a whole, suggesting that political ambition is not evenly distributed across all potential candidates. We discuss evidence from the first systematic study of political ambition in Britain, examining the question of who is interested in putting themselves forward for political office. We find patterns in the distribution of political ambition that help to explain why British political institutions do not look like the British people as a whole and include a gender gap, a social class gap, an education gap, a north–south divide, and a personality gap. We discuss the implications of our findings for political parties, arguing that they need to adjust practices of candidate recruitment in such a way that minimises the effects of these biases. 相似文献