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211.
Munchelou M. Gomonit BS Britni Skillman PhD Madeleine J. Swortwood PhD 《Journal of forensic sciences》2024,69(2):678-687
There has been burgeoning interest in psilocybin-use for the treatment of various neurological and neurodegenerative diseases. Psilocybin is mistakenly perceived as the principal pharmacologically active compound due to its high concentrations found in magic mushrooms; however, it is the prodrug of psilocin. Despite the expanding body of clinical research seeking to understand the pharmacodynamic/pharmacokinetic properties of psilocin, and its role in inducing dramatic changes to cognitive function, there has not been a corresponding increase in the development of sensitive analytical methods that can quantify psilocin in different biological fluids. Existing analytical methods have been developed using plasma, serum, and urine as the matrix of choice, but with the unknown blood-to-plasma ratio of psilocin, any pharmacokinetic conclusions drawn solely on plasma data may be misleading. Thus, the main objective of this study is to develop the first analytical method that utilizes SPE and LC–MS/MS to quantify psilocin in human whole blood. The SPE procedure yielded a high recovery efficiency (≥89%) with minimal matrix effects. The method was validated according to ANSI/ASB 036 guidelines. Linearity was between 0.7–200 ng/mL and encompassed previously reported ranges found in plasma/serum. Bias, within- and between-run precision for all quality controls met ANSI/ASB 036 acceptability criteria. Endogenous/exogenous interferences and carryover were negligible. Psilocin stability was assessed at 4°C over 48 h and was considered stable. Although a proof-of-concept study will need to be performed to characterize the method, this analytical workflow was able to detect and quantify psilocin in human whole blood at low limits of quantification. 相似文献
212.
Sara C. Zapico PhD Christian Stadler PhD Gabriela Roca PhD 《Journal of forensic sciences》2024,69(2):631-639
Despite current advances in body fluid identification, there are few studies evaluating the effect of environmental conditions. The present work assessed the detection of body fluids, blood, semen, and saliva, through lateral flow immunochromatographic (LFI) tests, exposed to tropical weather conditions over time, also evaluating the possibility of obtaining STR (short tandem repeat) profiles and identifying mitochondrial DNA (mtDNA) polymorphisms. Blood, semen, saliva samples, and mixtures of these fluids were deposited on polyester clothes and exposed to open-air tropical weather conditions for 1 month. The test versions from LFI (SERATEC®, Germany) Lab and crime scene (CS) used for the detection – one per each body fluid type – demonstrated that it is possible to identify body fluids and their mixtures up to 14 days after deposition. At 30 days, blood and semen were detected but not saliva. Full STR profiles were obtained from 14-day-old blood samples, and partial profiles were obtained from the remaining samples. It was possible to sequence mtDNA in the samples previously analyzed for STR profiling, and haplogroups could be assigned. In conclusion, this study demonstrated for the first time the possibility of body fluid identification and DNA profiling after exposure to tropical weather conditions for 1 month and also demonstrated the value of mtDNA analysis for compromised biological evidence. 相似文献
213.
JiLong Zheng PhD DeMin Huo MM HongYang Wen PhD QingFa Shang MM WenKai Sun MM ZiTong Xu MM 《Journal of forensic sciences》2021,66(1):356-364
The changes of postmortem corneal opacity are often used to roughly estimate the postmortem interval (PMI) in forensic practice. The difficulty associated with this time estimate is the lack of objective means to rapidly quantify postmortem corneal changes in crime scenes. This study constructed a data analysis model of PMI estimation and implemented an intelligent analysis system for examining the sequential changes of postmortem corneal digital images, named Corneal‐Smart Phone, which can be used to quickly estimate PMI. The smart phone was used in combination with an attachment device that provided a darkroom environment and a steady light source to capture postmortem corneal images. By segmenting the corneal pupil region images, six color features, Red (R), Green (G), Blue (B), Hue (H), Saturation (S), Brightness (V) and four texture features Contrast (CON), Correlation (COR), Angular Second Moment (ASM), and Homogeneity (HOM), were extracted and correlated with PMI model. The results indicated that CON had the highest correlation with PMI (R2 = 0.983). No intra/intersubject variation in CON values were observed (p > 0.05). With the increase in ambient temperature or the decrease in humidity, the CON values were increased. PMI prediction error was <3 h within 36 h postmortem and extended to about 6–8 h after 36 h postmortem. The correct classification rate of the blind test samples was 82%. Our study provides a method that combines postmortem corneal image acquisition and digital image analysis to enable users to quickly obtain PMI estimation. 相似文献
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215.
Determined to Die! Ability to Act Following Multiple Self‐inflicted Gunshot Wounds to the Head. The Cook County Office of Medical Examiner Experience (2005–2012) and Review of Literature 下载免费PDF全文
Ponni Arunkumar M.D. Aniello Maiese M.D. Giorgio Bolino PhD Lorenzo Gitto M.D. 《Journal of forensic sciences》2015,60(5):1373-1379
Cases of multiple (considered 2+) self‐inflicted gunshot wounds are a rarity and require careful examination of the scene of occurrence; thorough consideration of the decedent's psychiatric, medical, and social histories; and accurate postmortem documentation of the gunshot wounds. We present a series of four cases of multiple self‐inflicted gunshot wounds to the head from the Cook County Medical Examiner's Office between 2005 and 2012 including the first case report of suicide involving eight gunshot wounds to the head. In addition, a review of the literature concerning multiple self‐inflicted gunshot wounds to the head is performed. The majority of reported cases document two gunshot entrance wound defects. Temporal regions are the most common affected regions (especially the right and left temples). Determining the capability to act following a gunshot wound to the head is necessary in crime scene reconstruction and in differentiation between homicide and suicide. 相似文献
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217.
古代东亚秩序的形成与维系,同历史悠久的朝贡体系有着直接的关系。因此,要有效揭示古代东亚秩序的内在逻辑,应当批判地借鉴以历史主义见长的英国学派的分析方法,探讨朝贡体系的运行机制和内在本质。在古代,中国与朝鲜长时段的朝贡体系实质上是一种由中国主导并以其特有文化为基础、周边诸政权普遍认可和积极参与的特殊国际制度。就中国而言,完全独立的政治体制、自给自足的经济结构、等级制的世界观,使其对外部的需求仅限于其他政权对其至高无上地位的承认及其对相关制度安排的服从。而对周边诸政权来说,只有与实力超强的中国建立朝贡关系,它们才能获得合法性和安全保障,并取得与中国贸易的机会,从而实现生存与发展两大目标。由此可以得出如下结论:在缺乏文化共识的前提下,由地区强国主导,可以促成某种功能性的国际制度,使地区秩序得以维护,并在交往过程中反向促成文化认同。这一结论修正了英国学派关于共有文化与国际制度的关系的观点。 相似文献
218.
本文系统总结了自2002年底以来美韩同盟再定义的磋商过程,以及双方在驻韩美军基地调整、同盟内部军事安全角色分工、同盟的未来战略构想等三大焦点问题上所取得的实质性进展,认为通过区域扩展和内涵扩展的路径选择,美韩同盟再定义已基本实现利益平衡与威胁平衡的双重目标。通过对美韩全球性战略同盟关系的定位与确认,韩国也由此结束了数年来激烈的外交方向辩论,再度确立了中近期亲美的结盟外交路线,然而有关韩国对外战略的争论不会就此终结。 相似文献
219.
频繁的世界危机凸显出全球化时代下国际问题不可阻挡的跨国溢出与无序的世界治理秩序之间的深刻矛盾。从能源到气候变化问题,从大规模杀伤性武器的扩散到传染性疾病的防治,从国际金融系统的稳定到跨国宏观经济协调,政治、经济、安全问题复杂交织,世界越来越陷入动荡不安之中。当前处于世界新秩序重构的重要转型时期,中国不断增加的国家实力、声望和影响力赋予其在世界新秩序的构建中举足轻重的地位。本文旨在阐述中国在新秩序构建中的力量平衡、制度融合与理念引导作用。 相似文献
220.
Kobi Peled 《中东研究》2017,53(2):229-249
This article addresses the complex identity of Israel's Palestinian Arab citizens from an atypical perspective: through manifestations of their material culture. The cultural expressions that will be examined are objects from the past and objects that relate to the past, particularly to the rustic Palestinian life profoundly destabilized by the 1948 Arab–Israeli war. These objects, displayed in the homes of some of Israel's Arab citizens, are interpreted by contemplating their design, the design of their environment, their relationship to other objects, and their placement within the domestic sphere. Our study is animated by the desire to sketch a cultural portrait of Palestinian Arab Israelis, as well as by a methodological interest in interpretations based on a socio-architectural reading of objects.
This article reveals the various layers of meaning within these nostalgic displays in all their diversity, and will discuss at length their uniqueness, which is linked to the past traumas and present difficulties of Israel's Arabs. Our main purpose is to develop a line of thought whereby objects that express nostalgia are understood as the embodiment of a consciousness that is characteristic of the present. 相似文献