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931.
K Sawazaki T Yasuda D Nadano E Tenjo R Iida H Takeshita K Kishi 《Forensic science international》1992,57(1):39-44
We describe a method for obtaining specific and reproducible deoxyribonuclease I (DNase I) typing from liquid semen. Isoelectric focusing of the enzymes on polyacrylamide gel (IEF-PAGE, pH 3.5-5) was accomplished using a 0.5-mm thick gel. The separated isozymes were visualized by a new activity staining method, dried agarose film-overlay (DAFO). Pretreatment of semen samples with neuraminidase markedly enhanced the isozyme-band resolution and sensitivity. The method was simple and reliable, with high resolution and sensitivity. The DNase I types in semen samples were correlated with the types found in corresponding blood and urine samples. DNase I typing could therefore provide an additional discriminant characteristic in the forensic examination of semen. 相似文献
932.
Wendell E. Primus 《Journal of policy analysis and management》1989,8(1):23-34
By gathering and disseminating information, members of the Committee on Ways and Means, and committee staff under their supervision, have played an important role in informing the debate on poverty issues during the 1980s. In the face of President Reagan's 1981 budget program, which reduced spending in the major antipoverty programs, committee members targeted substantial resources to an ongoing, albeit ad hoc, education process. The process has not only provided evidence of the adverse impact of the 1981 reductions on the poor, but has also made new contributions to the understanding of poverty. Although many other factors have contributed to outcomes in poverty policy during the 1980s, it is clear that this education process played an important role in the successes achieved thus far; partial reversal of the 1981 reductions has been achieved, tax changes have been implemented which help low-income families, and a welfare reform bill has been enacted. 相似文献
933.
Kathleen M. Heide Ph.D. Eldra P. Solomon Ph.D. 《Journal of Police and Criminal Psychology》1997,12(2):35-41
The authors discuss a variety of forensic matters using cases in which they have been involved as experts. Pertinent issues
are illustrated in cases in which the offender is charged with homicide. The need for a comprehensive evaluation, the importance
of corroboration of the offender's statements, and the value of preparing a thorough report are discussed. 相似文献
934.
P. Cortivo M. Biasiolo C. Crestani C. Scorretti P. Benciolini 《Forensic science international》1984,24(1):65-68
The distribution of Tf phenotypes in the population of Padua was investigated by ultrathin-layer isoelectric focusing. In our sample (n = 618) nine phenotypes, Tf C1, C2, C3, C3?1, C2?1, C3?2, C1B, C2B and C1D, were observed and the following frequencies calculated: TfC1 = 0.77837; TfC2 = 0.1804; TfC3 = 0.03641; TfB = 0.0040; TfD = 0.0008. These gene frequencies have been compared to those found in other populations. Analysis of 101 mother-child pairs was in agreement with an autosomal codominant mode of inheritance. 相似文献
935.
936.
The applicability of isoelectric focusing in immobilized pH gradients for the Gc-subtyping in the forensic examination of bloodstains was tested. It is shown that due to the excellent separation of the Gc 1S and 1F bands subtyping of bloodstains can be done with high reliability. 相似文献
937.
K S Blisard C Martin G W Brown J E Smialek L E Davis P J McFeeley 《Journal of forensic sciences》1988,33(6):1457-1462
The causes of death of 53 severely to profoundly developmentally disabled patients who died in an intermediate care facility were reviewed. Respiratory disease, predominantly pneumonia and aspiration, accounted for 72% of deaths. Seven patients died of nonrespiratory causes, and in 8 patients, no cause of death could be determined, even after a complete autopsy or investigation. The median age at death was 20 years. The weights of these patients' organs at autopsy were lower than those for normal individuals of the same age. The lifespan of these severely impaired individuals continues to be significantly shortened, even with improved methods of care. 相似文献
938.
E S Traisman S Young B D Lifschultz H S Traisman I Benuck I Chasnoff 《Journal of forensic sciences》1988,33(1):267-271
This paper describes a case of a neonate with disseminated herpes simplex born to a 14-year-old asymptomatic mother. The infant's physical examination was normal at birth, and subsequent abnormalities were so subtle that infection was not recognized during life. Postmortem cultures of liver and spleen grew herpes simplex virus, and immunofluorescent direct antibody typing revealed Type 2. A cervical culture of the mother obtained after the infant's death was negative. 相似文献
939.
940.