The use of phosphate buffered saline for the recovery of cells and spermatozoa from swabs.

In the forensic science laboratory, the recovery of spermatozoa from vaginal swabs, or vaginal cells from penile swabs, can help determine if sexual intercourse may have taken place. There are several methods used to recover spermatozoa and cells from the swabs before visualisation on a microscope slide and most of these methods use water. Phosphate buffered saline (PBS) is a non-toxic solution used in many biological laboratories. Unlike water, PBS prevents cells rupturing or shrivelling up due to osmosis. This study demonstrates that PBS can be used for the extraction of spermatozoa and cells from swabs and that PBS does not affect subsequent DNA profiling.     

Judicial Procedures as Instruments of Political Control: Congress's Strategic Use of Citizen Suits

Citizen suit provisions, which give proregulatory interests access to the federal courts, can be used by Congress to increase the courts' role in regulatory policy. I analyze 284 environmental regulation bills reported favorably out of committee and show that committee support for citizen suits is a function of the committee's policy goals and the political context in which the bills are generated. These findings indicate that Congress deliberately uses judicial procedures as instruments of political control and that scholars examining judicial policymaking must include legislative goals in the list of explanatory factors.     

柔嫩艾美球虫杨凌株表面抗原SAG10基因的克隆与原核表达

从柔嫩艾美球虫第二代裂殖子总RNA中扩增EtSAG10基因,与pGEM-T Easy载体连接后转化大肠杆菌(E.coli)DH5α,筛选阳性克隆,扩增不含N端信号肽的编码序列,分别插入表达载体pET-32a( )和pMAL-c2X,转化至E.coliRosetta,以IPTG诱导表达。结果表明,pET-32a( )-EtSAG10在E.coliRosetta中的表达产物约占菌体总蛋白的43%,融合蛋白分子质量约为47 ku,以包涵体形式存在;而pMAL-c2X-EtSAG10在E.coliRosetta中表达的重组蛋白为可溶性,表达产物约占菌体总蛋白的35%,融合蛋白分子质量约为69 ku。以表达的可溶性EtSAG10重组蛋白100μg/只肌肉注射免疫雏鸡,攻虫后以盲肠病变计分、盲肠卵囊数(OPG)、相对增重率和抗球虫指数(ACI)评价,免疫组相对盲肠卵囊产量为47.7%,抗球虫指数由86.79提高至152.13。提示,重组表达的EtSAG10可诱导雏鸡产生一定的抗球虫免疫保护。     

The age of death: Appraising public opinion of juvenile capital punishment

Despite its original purpose to protect and rehabilitate wayward children, the juvenile system has grown more punitive and has embraced the use of harsher punishments, including execution, for juvenile offenders. Relatively little is known, however, about public attitudes toward the use of capital punishment for juveniles. This research explored the determinants of death penalty opinion, identified the minimum age at which respondents were willing to allow a juvenile to be put to death and examined the willingness of respondents to support an alternative sentence of life without the possibility of parole (LWOP). The results suggested that, while one-quarter of the sample was willing to execute juveniles who were fifteen and under at the time of the crime, there was less support for the execution of juveniles than of adults. In addition, of those who supported the use of the death penalty for juveniles, almost one-half would support LWOP as an alternative to the death penalty.     

中俄两国商业银行不良资产处置之比较

中国与俄罗斯商业银行都面临着大量银行不良资产的问题，为提高银行资产质量。两国采取了一系列改革措施。两国在不良资产形成原因、处置措施方面存在相似之处和差异，将二者进行比较，可对我国的商业银行处置不良资产提出有益的启示和借鉴。     

Der Begriff der „Landeskultur“ im deutschen Recht

Der Begriff der Landeskultur umfasst alle Maßnahmen zur Verbesserung der land- und forstwirtschaftlichen Nutzung des Bodens einschließlich der Erhaltung der Kulturlandschaft. Landeskultur ist somit alles Planen und Handeln mit dem Ziel, die Umwelt als gegebenes Naturraumpotential, insbesondere Wasser, Boden, Luft und die dazugehörigen Natur-, Kultur- und Sachgüter optimal zu erhalten, gestalten und rationell zu nutzen sowie dieses Potential mit bestmöglicher Qualität als natürliche und kulturelle Lebensgrundlage für die Allgemeinheit nachhaltig zu sichern. Landeskultur dient somit auch der Belebung und Werterhöhung der gebauten und gepflanzten Umwelt. Dies schließt auch und gerade die kulturelle Seite einschließlich Kulturlandschaften mit ein. Dabei ist es bis zur Überprüfung durch das BVerfG noch hinnehmbar, dass der Begriff „Landeskultur“ in Art. 89 Abs. 3 GG wegen der Mischkompetenz von Bund und Land vom BVerwG enger ausgelegt wird als in anderen Rechtsvorschriften.     

Toxicologic findings in the USS Iowa disaster.

The toxicologic results from the 47 victims of the explosion on the USS Iowa are presented. Good correlation between carboxyhemoglobin saturations and cause of death was found. There were no correlations between blood cyanide concentrations and causes of death. Volatile analysis suggested postmortem ethanol production rather than antemortem ethanol ingestion. No drugs except nicotine were detected in any of the victims.     

The establishment of the features of the blade of a stabbing-cutting weapon by the damages to human skin and to tricot clothing]

Presents forensic medical characteristics of the specific features of stabbed and cut injuries of human skin and jersey. Suggests a complex of signs that will help determine some structural parameters of a knife blade and the conditions of its interaction with the injured site of human body.     

Impact of tropical conditions on thin layer chromatography in analytical toxicology: high temperatures and moderate humidities.

The impact of high temperatures (24 to 39 degrees C) and low to moderately high humidities (20 to 70%) on the applicability of TLC systems for drug identification was studied during a 6 month climatologic cycle in Burkina Faso (West Africa). In general, the Rf values as observed on the plates were found to be substantially affected as compared with values obtained at temperate climates. Some TLC systems were more affected than others and the largest deviations of up to 30 Rf units were at low humidities. Tropical conditions also had a negative effect on the reproducibility of Rf values. However, when an Rf-correction procedure was applied, using reference mixtures of known drugs on each plate, accuracy as well as reproducibility of the resulting Rfc values were drastically improved and data thus corrected were found to be compatible with existing TLC data bases developed under moderate climatological conditions. The impact of high to extremely high humidities (70 to 100%) remains to be investigated.     

Law enforcement information sharing: A Florida case study

Many attempts have been made over the last several decades to improve communication among law enforcement agencies. This article is a case study of a “low-level” data sharing project in Florida that could serve as a national model. The Florida Law Enforcement Data Sharing Consortium is a partnership between the University of Central Florida and more than one hundred law enforcement agencies. It offers an inexpensive, yet technically advanced alternative to the proprietary data sharing model. Its distributed architecture was endorsed by the Markle Foundation, the 9/11 Commission, and the 2004 National Security Act. Civil liberties concerns raised by this and other types of data sharing projects are discussed.