Prospects for Sino-Russian Relations and China''s National Interests in the Next Decade

The next 5-10 years will be a pivotal period in which China will implement its "11th Five-Year Plan" and accelerate its construction of "a moderately prosperous society." It will also be an important period for Russia's resurgence. Sino-Russian relations are therefore facing new challenges and will be steadily developing. Under these new circumstances, clearly defining China's national interests in Sino-Russian relations will be an important prerequisite of ensuring the healthy and stable development of bilateral relations and of safeguarding China's peace and security. For the foreseeable future, China's national interests in Sino-Russian relations will be the further consolidation of mutual trust between the two countries and increasing Sino-Russian security cooperation so as to maintain peace and security in China's northern border areas. As the two countries are both in a phase of social transformation, they can nevertheless learn from each other throughout this process. China will continue promoting diversity of cultures and the multi-polarization of world. China should also exploit Sino-Russian economic cooperation and try to frame a joint strategy with Russia in order to achieve common development and prosperity. Consequently, the two countries will be able to march forward side by side along the path of economic reforms and structural adjustment." In the 21st Century, China and Russia not only need to maintain a good neighbor relationship and guarantee their joint security, but also build a closer cooperation in their common development.Studies, China Institutes of Contemporary International Relations.     

副溶血性弧菌LUXTM荧光PCR快速检测方法的建立

根据LUXTM荧光PCR技术原理,针对副溶血性弧菌toxR基因的保守序列,设计了特异的LUXTM荧光标记引物,通过优化反应条件与参数,建立了可快速检测副溶血性弧菌的LUXTM荧光PCR方法。结果显示,该方法高度敏感,对纯菌的检测低限达到每个反应体系7个菌(CFU),经6 h增菌培养后检测,对样品的检测低限(CFU)达到100/25 g;特异性强,对副溶血性弧菌2株标准菌株和10株参考菌株的检验均呈阳性,而对霍乱弧菌等28株非目标菌的检测均呈阴性;重复性好,定量检测的批内和批间变异系数均小于1%。应用此方法检测水产样品103份,检出阳性样品6份,与TaqMan荧光PCR和SN标准的检测结果完全一致。用此方法可在8 h内完成对样品中副溶血性弧菌的检测,其检测的快速性、敏感性和特异性与TaqMan荧光PCR技术相当,但检测成本更低。     

试析冷战后东盟与俄罗斯的关系

冷战结束后，俄罗斯与东盟的关系在政治、经济、安全等各方面都得到了较快的发展，这种发展态势是伴随俄罗斯外交政策的转变和东亚区域合作的兴起，以及国际、国内、双边一系列有利因素推动下形成的。但双边关系仍面临种种困难与挑战。以俄罗斯目前总体实力，在短期内还难以打破中美日在东南亚的力量平衡状态。     

小亚璃眼蜱4D8基因的克隆与原核表达

根据GenBank上登录的边缘革蜱4D8基因序列设计1对引物,采用PCR技术自半饱血小亚璃眼蜱雌性成蜱唾液腺cDNA表达文库中扩增获得了4D8基因;将其连入pMD18-T载体,构建重组克隆载体pMD18-Hyaa4D8.测序分析表明,克隆的小亚璃眼蜱4D8基因与边缘革蜱4D8基因的核苷酸序列的同源性为89.2%.将此片段定向亚克隆到原核表达载体pGEX-4T-1,构建重组原核表达载体pGEX-4T-1-Hyaa4D8,表达并纯化重组蛋白,通过免疫印迹试验鉴定该重组蛋白的生物学活性.结果显示,该重组蛋白是分子质量为45ku的融合蛋白,且表达产物能被半饱血小亚璃眼蜱雌性成蜱唾液腺抗原免疫兔血清识别.     

猪传染性胃肠炎病毒S基因抗原位点B与C之间片段的克隆与原核表达

将RT-PCR扩增的猪传染性胃肠炎病毒S基因抗原位点B与C之间的目的片段经克隆、双酶切后连接于表达载体,经酶切、PCR和测序鉴定的阳性重组质粒转化BL21(DE3),用IPTG诱导重组菌株表达其融合蛋白.结果显示,目的片段的大小为357bp,序列分析表明,S基因与其他猪传染性胃肠炎病毒相应基因具有很高的同源性;Western-blotting检测表明,分子质量约34ku的融合蛋白具有良好的反应原性.     

东南亚地区非传统安全合作机制架构与中国的策略思考

在东南亚地区的非传统安全合作中,存在3个层次的合作机制。东盟主导下的非传统安全合作机制,深化缓慢,执行乏力,主要扮演着合作的倡导者和规划者的角色;成员国间的双边或多边小范围合作机制是东盟合作计划的实施者,但合作领域单一,能力有限;区外西方大国主导的双边或多边合作机制,将传统安全与非传统安全合作相结合,发展迅速。这3个层次的非传统安全合作机制既相互支持,又相互竞争。面对东南亚非传统安全合作机制的独特架构,中国需进行策略思考。     

Top-Level Design for Chinese-Style Public Diplomacy against the Backdrop of Globalized Communication

<正>In the era of new media, the nature of being real-time and globalized of the public communication has posed unprecedented challenges to China’s public diplomacy as a whole. This is mainly manifested in the increasing obscurity of the boundary between domestic and foreign affairs. Any domestic event,     

政党政治与政治稳定——乌克兰案例研究

原苏联东欧国家的历史经验表明,在由传统社会迈向现代社会转型的进程中,都存在着大量的影响社会政治稳定的矛盾与问题。本文选取转轨国家中的乌克兰为案例,通过对政党政治涉及的三种主要关系来研究政治稳定。指出政党制度设计需要考虑到转轨前的政治社会和文化条件;缓慢发展的政党体系制约了政治稳定的实现;极化的政治文化限制了政治稳定的达成。     

日本血吸虫重组蛋白LHD-Sj23-GST诱导的细胞免疫应答和免疫保护效果

为探讨日本血吸虫重组蛋白LHD-Sj23-GST与3种不同类型佐剂(弗氏佐剂、ISA 206佐剂、ISA70M佐剂)配伍后免疫小鼠诱导的细胞免疫应答及免疫保护效果,应用流式细胞术检测并比较该重组蛋白配伍3种佐剂3次免疫后小鼠脾细胞中的CD4+、CD8+T细胞及细胞因子IFN-γ、IL-4、IL-10水平;3次免疫后小鼠人工攻击感染日本血吸虫尾蚴,6周后经肝门静脉灌注冲虫,计数各组平均虫体数并计算减虫率。与PBS对照组相比,LHD-Sj23-GST/FA和LHD-Sj23-GST/70M免疫组的CD4+T细胞显著升高(P<0.05),LHD-Sj23-GST/206免疫组未呈现明显变化;3种不同佐剂配伍LHD-Sj23-GST免疫组CD8+T细胞水平均显著降低(P<0.05);与PBS对照组相比,免疫组的IFN-γ及IL-10水平升高,而IL-4水平降低。LHD-Sj23-GST/FA、LHD-Sj23-GST/206和LHD-Sj23-GST/70M免疫组与PBS对照组相比,分别获得65.52%、51.72%和51.72%的减虫率;而相较于各自的佐剂对照组,则分别获得58.76%,26.31%,54.28%的减虫率。结果表明,用重组蛋白LHD-Sj23-GST配伍3种不同类型佐剂免疫小鼠,均刺激产生了偏向Th1型的细胞免疫应答,且都对小鼠诱导了较高的免疫保护作用。