Internal Validation of Human Mitochondrial DNA Quantification Using Real‐Time PCR

The quantity of mitochondrial DNA (mtDNA) template added for amplification and subsequent dye terminator reactions is critical for obtaining quality sequence data. Validation of a human mtDNA real‐time quantitative PCR (qPCR) assay demonstrated its high degree of reproducibility and precision as well as an extremely sensitive threshold of detection (0.0001 pg/μL or approximately six human mtDNA copies/μL). A study of 35 nonprobative bone and teeth evidence samples revealed that 20 pg of mtDNA template is recommended for successful HV1 and HV2 sequence analysis; however, as little as 0.013 pg can generate a full mtDNA profile when using enhanced amplification reactions. The assay can also detect PCR inhibition and is useful for identifying samples that may benefit from re‐purification. Overall, the assay is an excellent method to quantify mtDNA and is useful for determining the best analytical approach for successful sequencing.     

What does it mean to suffer loss? Haxton v Philips Electronics

In Haxton v Philips Electronics the Court of Appeal considered whether a widow could recover the diminution in value of her dependency claim following the defendant's tortious reduction of her life expectancy. The note outlines the development of the common law, demonstrating that Haxton is novel but not unorthodox, and tests whether Haxton's principles can provide a sound foundation for future cases. Positing three hypothetical scenarios, it argues that the disparity in outcome, rather than indicating a lack of unifying principle, as was suggested in Jobling v Associated Dairies, may be explained by combining Austin's division between primary and secondary rights with Gardner and Stevens' contributions as to how they are protected. Restitutio in integrum requires consideration of the reasons and values underlying the right in question and these are discernible in the jurisprudence. The note also considers whether Haxton could have been decided on the basis that a defendant should not profit from its own wrongdoing.     

A Criminological Approach to Explain Chronic Drunk Driving

The purpose of this paper is to use criminological theories to explain chronic drunk driving. There is little criminological research explaining recidivist drunk driving with criminological theories. Instead, most researchers posit that repeat drunk driving is explained as a byproduct of substance abuse. Although substance abuse is likely correlated to chronic drunk driving, theoretical explanations need to go further to understand a broader set of social and psychological predictors. Factor analysis and linear regression techniques are used to estimate the relationship between items from two assessment instruments with a number of drunken driving offenses. The sample consists of nearly 3,500 individuals on probation and parole in a Southwestern state. The findings support our contention that criminological frameworks are helpful to understand chronic DUI. We found significant results for volatility, antisocial friends, teenage deviance, and negative views of the law, while controlling for age, gender, marital status, and race. DUIs are a serious problem for the criminal justice system and understanding the individual level correlates of repetitive DUI is crucial for policy development. Further, chronic DUI offers criminologists an opportunity to determine the ability of criminological theories to explain this type of behavior.     

Of Race,Prison, and Perception: Seeking to Account for Racially Divergent Views on the Relative Severity of Sanctions

Several studies have found that offenders do not always perceive prison to be a harsher sanction than community-based punishments. Moreover, the literature shows that white offenders tend to estimate prison to be relatively more severe than do black offenders. The present study develops and tests eight possible explanations for the observed racial gap in perceptions. Relying on survey data from inmates in a large urban jail to establish sentencing preferences for black and white inmates, multivariate analyses show that the racial gap was attenuated but not eliminated by the explanations. This persistent racial difference in opinions of sanction severity is consistent with differential perceptions of criminal justice system fairness and merits additional research. The race gap has implications for theories on the effects of incarceration as well as sentencing practice.     

The religious conversion and race of a prisoner: Mock parole board members' decisions,perceptions, and emotions

Parole board members (PBMs) decide whether to release inmates on parole. Decisions may be affected by in‐group bias or stereotypes regarding religion and race. Two experiments investigated whether religious conversions/secular lifestyle changes and race affect mock PBMs' release decisions, emotions, and perceptions. Mock PBMs read a case file of an inmate who was eligible for parole and decided whether to grant parole. Study 1 manipulated whether the inmate had converted to Christianity or Islam, had a secular lifestyle change, or had no lifestyle change. Study 2 also varied race (African American or Caucasian). Race was not a significant factor, possibly because the manipulation was not strong enough to influence participants or because participants did not want to appear racist. Conversions to Islam and Christianity impacted the parole decision, and effects were mediated by believability of the conversion. Secular lifestyle changes affected release decisions and were mediated by perceptions of the inmate and beliefs about his likelihood of recidivism. Such inmates were the most likely to be released and were perceived most positively; their conversions were the most believable. Inmates who made no changes were perceived least positively, indicating that any lifestyle change is better than none. Importantly, no bias towards either religion (Islam, Christianity) was found. Furthermore, conversion type affected how scared PBMs were of the inmate, but this fear did not impact release decisions.     

Real interest rate persistence in South Africa: evidence and implications

The real interest rate is a very important variable in the transmission of monetary policy. It features in vast majority of financial and macroeconomic models. Though the theoretical importance of the real interest rate has generated a sizable literature that examines its long-run properties, surprisingly, there does not exist any study that delves into this issue for South Africa. Given this, using quarterly data (1960:Q2-2010:Q4) for South Africa, our paper endeavors to analyze the long-run properties of the ex post real rate by using tests of unit root, cointegration, fractional integration and structural breaks. In addition, we also analyze whether monetary shocks contribute to fluctuations in the real interest rate based on test of structural breaks of the rate of inflation, as well as, Bayesian change point analysis. Based on the tests conducted, we conclude that the South African EPPR can be best viewed as a very persistent but ultimately mean-reverting process. Also, the persistence in the real interest rate can be tentatively considered as a monetary phenomenon.     

Perceived Quality of Life and Health Complaints in Children Exposed to Intimate Partner Violence

Children 9 to 13 years old exposed to intimate partner violence (IPV) reported on their violence exposure, attachment to both parents, temperament (negative emotionality and emotion regulation), perceived quality of life, and health complaints. Half of the children perceived their quality of life as good and did not have recurrent health complaints. When controlling for socioeconomic status, health complaints were associated with higher IPV exposure and negative emotionality, whereas quality of life was associated with attachment security, higher capacity for emotion regulation, and lower negative emotionality. These results underscore the importance of increasing and supporting the capacity of children exposed to IPV to handle and express their emotions, as well as making school nurses and other primary care practitioners more attentive to IPV as a possible background factor in children’s health complaints.     

Navigating the role of the principal investigator: a comparison of four cases

Principal investigators are the lead actors on projects at the forefront of nascent technologies, yet few studies have explored the personal actions and experiences of PIs as they navigate their roles. I investigated principal investigators and their approach to new boundary spanning and entrepreneurial roles. Following a multiple case study methodology with a combination of interviews and observation, four PIs in nanotechnology related fields are explored in three dimensions: career and institutional alignment, boundary spanning activities and the tensions created in the still largely uncharted waters of nanotechnology commercialization. I found that these PIs actively sought organizational alignment that allowed them “to make things happen” while keeping harmony between the university and enterprise. The PIs demonstrated boundary-spanning activities, in particular a propensity for welcoming strangers into their labs in the hopes of finding new knowledge and opportunities, and practicing “good grantsmanship” to convert these new relations into collaboration. I found that the PIs managed tensions related to academic progression and lack of institutional support. Through this study, I offer researchers an opportunity to hear the voice of PIs on these topics and seek to contribute to our understanding of PIs as critical actors in the pursuit of science.