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61.
The Use of Laser Microdissection in Forensic Sexual Assault Casework: Pros and Cons Compared to Standard Methods 下载免费PDF全文
Sergio Costa M.Sc. Paulo Correia‐de‐Sá M.D. Ph.D. Maria J. Porto Ph.D. Laura Cainé Ph.D. 《Journal of forensic sciences》2017,62(4):998-1006
Sexual assault samples are among the most frequently analyzed in a forensic laboratory. These account for almost half of all samples processed routinely, and a large portion of these cases remain unsolved. These samples often pose problems to traditional analytic methods of identification because they consist most frequently of cell mixtures from at least two contributors: the victim (usually female) and the perpetrator (usually male). In this study, we propose the use of current preliminary testing for sperm detection in order to determine the chances of success when faced with samples which can be good candidates to undergo analysis with the laser microdissection technology. Also, we used laser microdissection technology to capture fluorescently stained cells of interest differentiated by gender. Collected materials were then used for DNA genotyping with commercially available amplification kits such as Minifiler, Identifiler Plus, NGM, and Y‐Filer. Both the methodology and the quality of the results were evaluated to assess the pros and cons of laser microdissection compared with standard methods. Overall, the combination of fluorescent staining combined with the Minifiler amplification kit provided the best results for autosomal markers, whereas the Y‐Filer kit returned the expected results regardless of the used method. 相似文献
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Larry J. Nelson Laura M. Padilla-Walker Sarah Badger Carolyn McNamara Barry Jason S. Carroll Stephanie D. Madsen 《Journal of youth and adolescence》2008,37(5):605-615
Many studies have documented the ways in which shyness can be a barrier to personal well-being and social adjustment throughout
childhood and adolescence; however, less is known regarding shyness in emerging adulthood. Shyness as experienced during emerging
adulthood may continue to be a risk factor for successful development. The purpose of this study was to compare shy emerging
adults with their non-shy peers in (a) internalizing behaviors, (b) externalizing behaviors, and (c) close relationships.
Participants included 813 undergraduate students (500 women, 313 men) from a number of locations across the United States.
Results showed that relatively shy emerging adults, both men and women, had more internalizing problems (e.g., anxious, depressed,
low self-perceptions in multiple domains), engaged in fewer externalizing behaviors (e.g., less frequent drinking), and experienced
poorer relationship quality with parents, best friends, and romantic partners than did their non-shy peers.
Larry J. Nelson is an Associate Professor in the School of Family Life at Brigham Young University. He received his Ph.D. in 2000 from the University of Maryland, College Park. His major research interests are in social and self development during early childhood and emerging adulthood. Laura M. Padilla-Walker is an Assistant Professor in the School of Family Life at Brigham Young University. She received her Ph.D. in 2005 from the University of Nebraska—Lincoln. Her major research interests center on the parent-adolescent relationship as it relates to adolescents’ moral and prosocial behaviors and internalization of values. Sarah Badger received her Ph.D. in 2005 from Brigham Young University. Her major research interests are marriage formation and development as well as emerging adulthood and marriage readiness. Carolyn McNamara Barry is an Assistant Professor of Psychology at Loyola College in Maryland. She received her Ph.D. in 2001 from the University of Maryland, College Park. Her major research interests are in social and self development during adolescence and emerging adulthood. Jason S. Carroll is an Associate Professor in the School of Family Life at Brigham Young University. He received his Ph.D. in 2001 from the University of Minnesota. His major research interests are in marriage formation and development as well as emerging adulthood and marriage readiness. Stephanie D. Madsen is an Associate Professor of Psychology at McDaniel College. She received her Ph.D. in 2001 from the Institute of Child Development, University of Minnesota. She is particularly interested in how relationships with significant others impact child and adolescent development. 相似文献
Larry J. NelsonEmail: |
Larry J. Nelson is an Associate Professor in the School of Family Life at Brigham Young University. He received his Ph.D. in 2000 from the University of Maryland, College Park. His major research interests are in social and self development during early childhood and emerging adulthood. Laura M. Padilla-Walker is an Assistant Professor in the School of Family Life at Brigham Young University. She received her Ph.D. in 2005 from the University of Nebraska—Lincoln. Her major research interests center on the parent-adolescent relationship as it relates to adolescents’ moral and prosocial behaviors and internalization of values. Sarah Badger received her Ph.D. in 2005 from Brigham Young University. Her major research interests are marriage formation and development as well as emerging adulthood and marriage readiness. Carolyn McNamara Barry is an Assistant Professor of Psychology at Loyola College in Maryland. She received her Ph.D. in 2001 from the University of Maryland, College Park. Her major research interests are in social and self development during adolescence and emerging adulthood. Jason S. Carroll is an Associate Professor in the School of Family Life at Brigham Young University. He received his Ph.D. in 2001 from the University of Minnesota. His major research interests are in marriage formation and development as well as emerging adulthood and marriage readiness. Stephanie D. Madsen is an Associate Professor of Psychology at McDaniel College. She received her Ph.D. in 2001 from the Institute of Child Development, University of Minnesota. She is particularly interested in how relationships with significant others impact child and adolescent development. 相似文献
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This paper argues that transitional justice needs to adopt a participatory approach to achieve longer-term sustainability, shifting away from the top-down 'one-size-fits-all' approach to allow 'voices from below' to be heard and heeded. It critiques dominant interpretations of key transitional justice concepts, and links them to the difficulties of post-conflict transitional justice in a range of violently divided societies. Popular participation and local agency, it is argued, is necessary to achieve ends identified in much transitional justice discourse, and to embed mechanisms for the creation of sustainable peace. A Northern Ireland initiative (the Ardoyne Commemoration Project) will be explored in-depth, illustrating how a bottom-up 'truth-telling' process can make a significant contribution to transitional justice and casting doubt on the validity of the deference to legal dominance in current policy and practice. The paper recommends that knowledge available in development studies and participatory theory be applied more clearly in debates and approaches in transitional justice. 相似文献
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This paper examines the noncustodial parent earned income tax credit (NCP EITC), a new type of credit recently enacted in New York and Washington, D.C., and proposed by Senator Bayh and then‐Senator Obama in 2007. The NCP EITC offers an earned income tax credit to low‐income noncustodial parents who work and pay their full child support. This paper provides background information about the policy and presents national estimates of eligibility and benefits for an NCP EITC under three alternative policy scenarios. It also discusses several key design and implementation issues. © 2010 by the Association for Public Policy Analysis and Management. 相似文献
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Increasingly development theorists and practitioners view NGOs as catalysts of sustainable development. NGOs have been regarded as champions of democratisation and promoters of new ways of engaging in politics, with considerable influence on the development of civil society and new partnerships in environmental and social advocacy. This article analyses the ways in which Costa Rican environmental NGOs (ENGOs) engage in politics, by focusing on their perceptions of their roles in environmental governance and in representation of civil society. The results of this study suggest that the ENGOs' ways of engaging in politics differ little from traditional forms of governance, while their conceptions of engaging in politics without being political are novel. While most ENGOs had no clear conception of the stakeholders whom they were supposed to be representing, the notion of representativeness is complex and should be revisited. 相似文献