蓝舌病病毒NS2蛋白单克隆抗体的制备及其应用

本研究旨在制备针对蓝舌病病毒(bluetongue virus,BTV)非结构蛋白NS2的单克隆抗体,进而用于C-ELISA鉴别BTV自然感染动物与BTV灭活疫苗免疫动物。通过原核表达BTV NS2蛋白的高保守多肽(1~228 aa)来制备抗原,并通过免疫BALB/c小鼠制备抗BTV NS2蛋白的单克隆抗体,结果,获得1株分泌单克隆抗体的小鼠杂交瘤细胞,命名为BTV-2A4。鉴定结果显示,该单克隆抗体的亚类/亚型为IgG1/κ;其抗原表位在NS2蛋白的91~138 aa区间;能特异性地与1~24型BTV的NS2蛋白反应;可应用于Western-blot、间接免疫荧光、间接ELISA及C-ELISA技术。C-ELISA应用的试验数据表明,BTV疫苗免疫动物及未被BTV感染动物的血清中检测不到NS2抗体,而BTV感染动物的血清中大多可被检出NS2抗体。     

The impact of stakeholder engagement on local policy decision making

Policy Sciences - We investigate how grassroots stakeholder engagement in municipal meetings shapes the decision making of local elected officials (LEOs) by examining the choices LEOs in New York...     

国际特许经营中商标独占使用权保护的困境与选择——由一起商标商品平行进口案例引发的思考

国际特许经营中商标商品的平行进口造成了对特许经营人商标独占使用权的侵害。本文着力分析了这一具体平行进口模型中平行进口行为的性质,试图探求一种被侵害知识产权的救济方式。在效益不同而权利发生冲突时应遵循效益最大化原则。如何通过保护特许经营人之商标权来实现利益最大化或者至少防止利益失衡应成为我们要思考的一个问题。     

城市高龄老人社会化养老服务问题研究——基于南京市G区的实证调查

高龄老人由于生理机体、经济能力、心理承受力等特征的弱化．与中低龄老人有着不同的服务需求。基于南京市G区的实证调查，将两群体有关生活照料、医疗卫生、精神慰藉和社会参与等四方面的服务供求情况进行对比研究，总结高龄老人对于养老服务需求的殊异性，进而有针对性地提出完善城市高龄老人社会化养老服务的对策建议。     

Urban Governance And Policy Networks: On The Urban Political Boundedness of Policy Networks. A French Case Study

The paper analyses the changes within two policy domains in one French city. The aim is not so much to demonstrate the role of policy networks but to stress the importance of their articulation within a given political space. The paper does not conclude on the emergence of a new type of network governance but rather shows how urban political élites have deliberately encouraged the formation of policy net-works in Rennes. That strategy makes sense in their attempt to strengthen an urban collective actor. Policy networks therefore should not be analysed autonomously as their impact is largely shaped by the interest and collective goals of the existing regime of governance in Rennes.     

新农村建设背景下农村地区遗传资源的保护和利用

遗传资源是上世纪末以来人们日益关注的宝贵资源之一,可以分为植物遗传资源、动物遗传资源和人体基因资源。我国是遗传资源保护大国,大量的遗传资源存续于农村地区。当前的遗传资源面临流失和被剽窃的局面,存续状况不容乐观。新农村建设为农村地区的遗传资源保护工作明确了指导思想,提供了良好的工作环境和有效的工作方式。因此,完善法制建设,发挥基层组织作用,鼓励科学合作研究,提高农民素质和保护意识,探索遗传资源利用的商业化运作模式,是加强农村地区遗传资源保护和利用的有效途径。     

Structural budget deficits in the federal government: A theoretical perspective

Survey data show managerial motivations and behaviors are statistically linked to numerous aspects of computer use in public agencies. Motivations to enhance service quality and work environments seem to be particularly influential in using technology to enhance productivity. Empirical research in public administration should be attentive to individuals’ perceptions regarding the impacts of computers. Normative deliberation, however, is needed to determine the appropriate role of managers.     

美国信息安全战略发展研究

网络与信息系统安全已经引起了全世界共同关注,美国在此领域一直走在世界前列。伴随世界经济、文化的日益融合,美国信息安全立法及政策方面的丰富经验为我国信息安全战略的制定和完善提供了可资借鉴的蓝本。解读美国信息安全战略的演变轨迹、组织体系及其特点,结合我国信息安全保障立法现状,分析当前我国信息安全立法的主要着力点,旨在构建有中国特色的信息安全法律体系。     

安源对新型人民军队创建的历史贡献

安源是湘赣边界秋收起义的策源地和主要爆发地之一,以安源工人为主力的工农革命军第一师第二团在秋收起义中作出了突出贡献。安源是毛泽东最早创建新型人民军队的实践地,安源和安源工人在创建这支新型人民军队和发展壮大这支新型军队的历史进程中,发挥了重要作用。     

Substance Use as a Longitudinal Predictor of the Perpetration of Teen Dating Violence

The prevention of teen dating violence is a major public health priority. However, the dearth of longitudinal studies makes it difficult to develop programs that effectively target salient risk factors. Using a school-based sample of ethnically diverse adolescents, this longitudinal study examined whether substance use (alcohol, marijuana, and hard drugs) and exposure to parental violence predicted the perpetration of physical dating violence over time. 1,042 9th and 10th grade high schools students were recruited and assessed in the spring of 2010, and 93 % of the original sample completed the 1-year follow-up in the spring of 2011. Participants who had begun dating at the initial assessment and who self-identified as African American (n = 263; 32 %), Caucasian (n = 272; 33 %), or Hispanic (n = 293; 35 %) were included in the current analyses (n = 828; 55 % female). Slightly more than half of the adolescents who perpetrated dating violence at baseline reported past year dating violence at follow-up, relative to only 11 % of adolescents who did not report perpetrating dating violence at baseline. Structural equation modeling revealed that the use of alcohol and hard drugs at baseline predicted the future perpetration of physical dating violence, even after accounting for the effects of baseline dating violence and exposure to interparental violence. Despite differences in the prevalence of key variables between males and females, the longitudinal associations did not vary by gender. With respect to race, exposure to mother-to-father violence predicted the perpetration of dating violence among Caucasian adolescents. Findings from the current study indicate that targeting substance use, and potentially youth from violent households, may be viable approaches to preventing the perpetration of teen dating violence.