La commission parlernentaire: mécanisrne d'imputabilitéà l‘égard des sous-ministres et des dirigeants d'organismes

Sommaire: Plusieurs parlementaires, divers analystes et deux commissions d'étude récentes, l'une au palier fédéral (Lambert) et l'autre au Québec (Bisaillon), ont traité de la possibilité d'implanter un régime d'imputabilité pour les administrateurs publics, particulièrement les sous-ministres et les dirigeants d'organismes. Les modifications apportées, au début de la présente année, aux règles de procédure de l'Assemblée nationale vont permettre une certaine expérimentation à cet égard. Une telle réforme améne l'auteur à examiner diverses contraintes qui pourraient influencer et même jouer contre son implantation, notamment: les principes de la responsabilité et de la solidarité ministérielles, l'évolution des rapports entre le gouvernement et l'administration, les différences de statut entre les sous-ministres et les dirigeants d'organismes et les précédents relatifs à la participation des sous-ministres et des dirigeants d'organismes aux travails de l'Assemblée. Ces contraintes sont assurément sérieuses car elles remettent en cause des éléments majeurs du système politique. Reste à savoir si, comme cela se produit souvent, les hommes politiques réussiront encore une fois à concilier ce qui paraît incompatible. Abstract: Several members of Parliament, various analysts and two recent commissions of inquiry, one federal (Lambert Commission) and the other from Quebec (Bisaillon), have studied the possibility of implementing an accountability system for public administrators, particularly deputy ministers and chief executive officers of agencies. Amendments brought forth earlier this year to the procedures of the National Assembly of Quebec will allow for experimentation in that area. Such a reform leads the author to examine various constraints in its implementation; in particular, the principles of ministerial responsibility and soolidarity; the evolution of relationships between government and administration; the status differences between deputy ministers and chief executive officers of agencies; the precedents concerning the participation of deputy ministers and chief executive officers in the work of the Assembly. Those constraints are of great importance since they challenge some major elements of the political system. It remains to be seen whether politicians will succeed once again in reconciling what appears to be incompatible.     

Distribution of Y chromosome lineages in Jerba island population

We have analysed Y chromosome polymorphism on six STR markers (DYS19, DYS389I, DYS390, DYS391, DYS392, and DYS393) and eight classical UEP markers (SRY10831a, YAP, SRY4064, M2, 92R7, M9, SRY2627 and 12f2) in three distinct ethnical, linguistic and cultural groups of Jerba island (Berbers, Arabs and a Jerban group of Sub-Saharan origin). Fst genetic distance and principal co-ordinate analysis based on STR haplotype frequencies, showed a genetic differentiation between the three Jerban groups and a genetic relationship between Jerban Berbers and Mozabites (a well defined Berber group in Algeria). Compound use of UEP and STR markers have increased discriminatory capacity. The detection of the most common haplotype (H9) in both Berbers and Mozabites may be useful in forensic special cases.     

Identification of alprazolam in hair in two cases of drug-facilitated incidents

The use of a drug to modify a person's behaviour for criminal gain is not a recent phenomenon. However, the recent increase in reports of drug-facilitated crimes (sexual assault, robbery) has caused alarm by the general public. Among the drugs that can be used, alprazolam (Xanax), an anxiolytic benzodiazepine, has been seldom observed. To document two cases involving this drug, we have developed an approach based on hair testing by LC-MS/MS. After pH 8.4 buffer incubation and extraction with methylene chloride/diethyl ether (80/20, v/v), hair extracts were separated on a XTerra MS C18 column using a gradient of acetonitrile and formate buffer. Alprazolam and diazepam-d5, used as internal standard, were detected by electrospray tandem mass spectrometry. In the first criminal case, alprazolam tested positive in two consecutive 2 cm hair segments at 4.9 and 2.4 pg/mg, from a 12-year-old girl, assaulted by her father who had sedated her three or four times. In the other case, alprazolam was detected in four consecutive 1cm hair segments at 3.1-0.4 pg/mg, obtained from an adolescent who had been forced to prostitute herself.     

Women's response to intimate partner violence

The responses of women to a situation of abuse by their partner has hardly been addressed in the literature. Using a self-administered, anonymous questionnaire, 400 women attending three practices in a primary health care center in Granada (Spain) were studied. The women's response to abuse was used as a dependent variable. Sociodemographics, intensity, duration, and combination of the types of abuse were used as independent variables. Lifetime prevalence of any type of partner abuse was 22.8%. Of abused women, 68% showed an active response, attempting to resolve the situation mainly through separation (58.2%). The factors independently associated with a woman's active response were being separated, widowed, or divorced; reporting a greater intensity of abuse; and being younger than age 35 years. The results of this study show that a large majority of abused women in Spain try to resolve their situation, and that they are not passive victims.     

Development of a capillary zone electrophoresis method including a factorial design and simplex optimisation for analysis of amphetamine, amphetamine analogues, cocaine, and heroin

A capillary zone electrophoresis (CZE) method was developed for the analysis of amphetamine and 13 amphetamine analogues. A full factorial design was used to screen for important design variables (i.e. carrier electrolyte concentration, pH, and separation temperature), and a modified simplex was employed in a final optimisation step. The resolution values of the target compounds were used as responses in the screening and optimisation phases. This approach made it possible to control the effects of the design variables on the separation of the target compounds. The best results were obtained using a 100mM Tris/phosphate buffer (pH 3.1) at a separation temperature of 10 degrees C, and the analysis time was 23 min under these conditions. After slight modification, the method also enabled baseline resolution of the most commonly encountered amphetamine derivatives, as well as cocaine and heroin, within 7 min. There was a linear relationship between peak area and concentration for all substances, with correlation coefficients in the range of 0.9975-0.9999. Moreover, the technique was repeatable and exhibited relative standard deviation (R.S.D.) values in the ranges of 0.01-0.11% and 0.54-1.60% for relative migration time and corrected peak area, respectively. Lastly, the method was successfully applied to analyse street samples.     

Y-chromosome STRs in an Antioquian (Colombia) population sample

Haplotype data were obtained from a sample of 777 unrelated male individuals from Antioquia Department (Colombia), for eight Y-chromosome STRs (DYS19, DYS385, DYS389 I, DYS389 II, DYS390, DYS391, DYS392 and DYS393). A total of 442 different haplotypes were identified of which 334 were represented only once in the database and the most frequent haplotype was found in 32 individuals. A high haplotype diversity was found (99.45%). Genetic distances were calculated using previously published haplotype data and the lowest values were found for the comparisons with samples of lberian origin.     

Automated comparison of firearm bullets

Fired bullets bear striation marks that can be thought of as a "fingerprint" left by the firearm. This new comparison approach is based on an automated extraction of a "signature" encompassing the relevant marks from an image. To this end, multiple pictures of the bullet are recorded first by using different illumination patterns, and a high quality image is generated by means of fusion techniques. After a preprocessing, the image intensities are filtered along the striations direction, yielding a compact representation of the marks. A non-linear filter selects the striae of interest. The actual comparison takes place by cross-correlating the signatures obtained this way. Finally, an assessment strategy is proposed to objectively evaluate the performance of the system. As demonstrated with an image database of real bullets, the proposed approach outperforms a state-of-the-art commercial system.     

Nuclear and mitochondrial DNA quantification of various forensic materials

Due to the different types and quality of forensic evidence materials, their DNA content can vary substantially, and particularly low quantities can impact the results in an identification analysis. In this study, the quantity of mitochondrial and nuclear DNA was determined in a variety of materials using a previously described real-time PCR method. DNA quantification in the roots and distal sections of plucked and shed head hairs revealed large variations in DNA content particularly between the root and the shaft of plucked hairs. Also large intra- and inter-individual variations were found among hairs. In addition, DNA content was estimated in samples collected from fingerprints and accessories. The quantification of DNA on various items also displayed large variations, with some materials containing large amounts of nuclear DNA while no detectable nuclear DNA and only limited amounts of mitochondrial DNA were seen in others. Using this sensitive real-time PCR quantification assay, a better understanding was obtained regarding DNA content and variation in commonly analysed forensic evidence materials and this may guide the forensic scientist as to the best molecular biology approach for analysing various forensic evidence materials.