A Concentrated Hydrochloric Acid-based Method for Complete Recovery of DNA from Bone

The successful extraction of DNA from historical or ancient animal bone is important for the analysis of discriminating genetic markers. Methods used currently rely on the digestion of bone with EDTA and proteinase K, followed by purification with phenol/chloroform and silica bed binding. We have developed a simple concentrated hydrochloric acid-based method that precludes the use of phenol/chloroform purification and can lead to a several-fold increase in DNA yield when compared to other commonly used methods. Concentrated hydrochloric acid was shown to dissolve most of the undigested bone and allowed the efficient recovery of DNA fragments <100 bases in length. This method should prove useful for the recovery of DNAs from highly degraded animal bone, such as that found in historical or ancient samples.     

The Government as Litigant: Further Tests of the Case Selection Model

We develop a model of the plaintiff's decision to file a lawsuitthat has implications for how differences between the federalgovernment and private litigants translate into differencesin trial rates and plaintiff win rates at trial. Our case selectionmodel generates a set of predictions for relative trial ratesand plaintiff win rates, depending on the type of case and whetherthe government is defendant or plaintiff. To test the model,we use data on about 474,000 cases filed in federal districtcourt between 1979 and 1994 in the areas of personal injuryand job discrimination, in which the federal government andprivate parties work under roughly similar legal rules. We findbroad support for the predictions of the model.     

Severe personality disorder,treatment engagement and the Legal Aid,Sentencing and Punishment of Offenders Act 2012: what you need to know

Empirical research has demonstrated a link between legal coercion and treatment engagement following conviction among those with severe personality disorder. Legal coercive pressures were often applied by the Indeterminate Sentence for Public Protection (IPP), until it was replaced by the Extended Determinate Sentence by the Legal Aid, Sentencing and Punishment of Offenders Act 2012. In this paper, it is proposed that use of the new determinate sentence will lessen motivation for treatment engagement. One effect of treatment refusal may be greater reliance by the Secretary of State for Justice on his jurisdiction to transfer prisoners due for release to secure hospital transfers under the Mental Health Act 1983. Not only will this risk posturing undermine the principal aim of the Offender Personality Disorder Implementation Pathway to improve treatment engagement among the target group, it will also have negative implications for medical practitioners working in secure forensic hospitals. To demonstrate what is at stake, the paper briefly recapitulates empirical findings familiar to readers of the journal, before drawing on original unpublished data.     

Managing rural landscapes in the context of a changing climate

Global competition for natural resources is intense and the supply of those resources is increasingly more constrained by climate variability and change. Governments and international development agencies have the dual responsibility to meet the socio-economic needs of the poorest and most vulnerable people while preserving and enhancing their natural capital. These responsibilities often are at odds with each other and different stakeholder groups have prioritised one over the other. This paper suggests that the landscape approach provides a solution for stakeholders to achieve climate change mitigation, adaptation, and poverty reduction goals, though not without some trade-offs.