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121.
Richard Kristinsson M.S. ; Sarah E. Lewis B.S. ; Phillip B. Danielson Ph.D. 《Journal of forensic sciences》2009,54(1):28-36
Abstract: Denaturing high-performance liquid chromatography (DHPLC) was evaluated as a sequencing-independent means of detecting the presence of sequence differences in pair-wise mixtures of nonconcordant amplicons of human mitochondrial DNA (mtDNA). A total of 920 pair-wise combinations of HV1 and HV2 mtDNA amplicons from 95 individuals were assayed by DHPLC for sequence concordance/nonconcordance. For the 72 combinations of amplicons from different individuals who shared identical DNA sequences, DHPLC assays consistently indicated sequence concordance between the samples. This was in 100% agreement with sequencing data. For the 849 combinations of amplicons which differed in sequence, DHPLC detected the presence of sequence nonconcordance in all but 13 assays to yield 98.5% concordance with sequencing. Thus, DHPLC can be used to detect a diversity of sequence differences (transitions, transversions, insertions, and deletions) in the mtDNA D-loop. Accordingly, DHPLC may have utility as a presumptive indicator of mtDNA sequence concordance samples, as a screen for heteroplasmy/situational mixtures, and as a means for the physical fractionation of the individual contributors to an mtDNA mixture prior to sequencing. 相似文献
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Investigations into the causes and effects of parallel importinghave concentrated on price discrimination, but arbitrage canalso occur on non-price dimensions. Using a natural experimentin the New Zealand film distribution industry between May 1998and November 2001, we examine the effect of parallel importingon quality as it relates to the timing of the availability offilm media. We demonstrate that (a) cinema revenues were underminedas consumers substituted viewing films on parallel importedDVDs for the cinema format and (b) that studios responded tothe threat of parallel imported DVDs by bringing forward therelease of films into New Zealand cinemas. The reduced delaybetween US and New Zealand cinematic release dates is shownto be consistent with the introduction of competition when timingis a dimension of quality and choice. We conclude that parallelimportation of DVDs almost certainly resulted in a net increasein welfare in New Zealand. 相似文献
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Eric Lewis 《International Journal for the Semiotics of Law》2006,19(3):293-309
This essay investigates the interconnections between early Greek cosmology and legal theory. In particular, it demonstrate that spatial and legal concepts developed hand in hand, and that it would be mistaken to assume that legal terminology in cosmology, and/or spatial terminology in legal texts, are best read metaphorically. This state of affairs may be contrasted with contemporary legal theory, which often employs spatial terms metaphorically. The principal authors I␣consider are Solon, Anaxamander, Heraclitus and Parmenides. The essay concludes that justice and space/place were, for the Early Greeks, often equated—that distinct differing meanings for the two were not yet clearly established, and so, in a sense, there was both a spatial component to law, and a legal component to space. 相似文献
127.
Gail Lewis 《Feminist Review(on-Line)》2005,79(1):5-19
The article explores the textual construction of gender categories in the political discourse of Simón Bolívar by means of a close critical reading of his seminal writings made public between 1812 and 1820. The historical and political processes known as Latin American independence constitute a moment of radical transformation. It was during this period that the questions of political rights, nationality and citizenship were most open to debate throughout the continent. The article shows how the category woman is constructed ambiguously in Independence/anti-colonial discourse, how gender is employed to create hierarchical systems of social organization to legitimate the exercise of power by an elite of white creole men and how myth is deployed in order to reinforce gender hegemonies. It will be shown that in Bolívar's writings colonial relations are recast as family relations and political independence from Spain legitimated in terms of sexual difference and masculine domination. 相似文献
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This study investigates the effects of federal policy and budgetchanges during the Reagan years as well as the revenue strategiesof governmental and private, nonprofit agencies in Stamford,Connecticut. Systematic examination of agency budgets and interviewswith agency directors in 1982 found widespread anxiety aboutpossible revenue decline, but few departures from traditionalrevenue strategies. In Stamford, federal changes produced revenueproblems for only a few agencies, most of which went about "businessas usual" and did not experience a budget crisis. This situationremained unchanged in late 1985. 相似文献
130.
Lewis Anthony Dexter 《Society》1993,30(4):63-64
He has worked for various political organizations and governmental offices. Among his books are American Business and Public Policy (with Raymond Bauer); Tyranny of Schooling: An Inquiry into the Problem of Stupidity;and Representation Versus Direct Democracy.He is at work on a paper on loyalty and reform as sources of corruption. 相似文献