A minimally destructive technique for sampling dentin powder for mitochondrial DNA testing

The purpose of this paper is to present the horizontal sectioning technique used by odontologists at the Central Identification Laboratory to sample dentin for mtDNA analysis. From the perspective of DNA testing, anthropologists and odontologists at the Central Identification Laboratory work with ancient remains. In many instances, the lack of comprehensive antemortem records, the potential for fragmentation and commingling, and environmental exposure makes the use of traditional forensic identification techniques difficult or impossible. Teeth are highly resistant to environmental degradation and are an excellent source of mitochondrial DNA (mtDNA). This technique is simple, quick, and relatively conservative, allowing for preservation of the majority of the external portion of the tooth structure.     

Characterization and validation studies of powerPlex 2.1, a nine-locus short tandem repeat (STR) multiplex system and penta D monoplex

In order to increase the power of discrimination for human identification purposes, a nine-locus short tandem repeat (STR) multiplex, the GenePrint PowerPlex 2.1 system (PowerPlex 2.1) developed by Promega Corporation and a separate pentanucleotide-repeat locus, Penta D, were tested. This megaplex system includes the highly polymorphic loci FGA, TPOX, D8S1179, vWA, Penta E, D18S51, D21S11, TH01, and D3S1358 and may be used in combination with the eight-locus STR multiplex, the GenePrint PowerPlex 1.1 system (PowerPlex 1.1) that has been previously developed. Three of the loci, TPOX, TH01 and vWA, have been included in both systems for quality control purposes. As with PowerPlex 1.1, PowerPlex 2.1 is also based on a two-color detection of fluorescent-labeled DNA products amplified by polymerase chain reaction (PCR) and provides a valuable tool for accurate and rapid allele determination. The primer sequences used in the PowerPlex 2.1/Penta D system are also presented in this report. To meet the "Quality Assurance Standards for Forensic DNA Testing Laboratories" (FBI), we tested the efficiency and reproducibility of the PowerPlex 2.1/PentaD system by several validation studies that were conducted as a joint project among seven laboratories. Validation tests included concordance studies, sensitivity, and species specificity determination, as well as performance in forensic and environmentally impacted samples. The results produced from these tests demonstrated the consistency and reliability of the PowerPlex 2.1/Penta D system.     

Surveying Research on Parliament and Parliamentary Oversight of the Public Sector

This presentation begins by offering a brief overview of the existing research into Parliament and Parliamentary Oversight of the Public Sector. It highlights the work of those who are experts in the area and concludes by identifying areas which would benefit from further research.     

Political Appointments, Civil Service Systems, and Bureaucratic Competence: Organizational Balancing and Executive Branch Revenue Forecasts in the American States

Scholarship on executive politics provides conflicting views about whether staffing administrative agencies through politicized or (politically) autonomous means is the best method for maximizing bureaucratic competence. We offer a theoretical account which maintains that obtaining a proper balance between both types of personnel systems across the supervisory and subordinate levels of an organization will best foster bureaucratic competence. We evaluate our organizational balancing thesis using data on executive branch general revenue fund forecasts in the American states from 1987 to 2002. States with a combination of politically appointed agency executives and merit-selected subordinates generally provide more accurate revenue forecasts than states that possess uniformly politicized personnel selection systems. Conversely, states with a combination of department head–appointed executives and subordinates chosen from an at-will system (i.e., nonmerit) produce more accurate forecasts than states with uniformly autonomous personnel selection systems. Our statistical findings underscore the positive consequences associated with balancing politicized and autonomous means of selecting personnel within hierarchies of political organizations.     

Contesting Feminist Orthodoxies

This article is concerned with the construction of feminist literary studies in the last twenty years and points out how we have created a literary history which is both selective and schematic. It suggests that we should be more critically aware of what we are constructing, how we are constructing it and of the political consequences of those constructs. It stresses three critical modes which might help us to complicate our history: a greater awareness of institutional contexts, a concern with empirical detail, and an ongoing analysis of the cultural and political significance of feminist literary practice. This article briefly applies these critical modes in a survey of eleven introductions to feminist literary studies - introductions which feature frequently and influentially in the teaching situation. The final section focuses on the key problem of inclusion and exclusion. Considering arguments from Third World feminism and postmodernist feminism, the study concludes that white, academic feminists should confront the privilege of their own inclusion as a necessary spur to political action.     

The New Federalism and Substate Regionalism: Changing Perceptions of Rural Officials

This study examines the attitudes of officials in rural governmentsabout regional commissions. Survey data from a sample of Georgiaand Michigan respondents were collected in the 1970s and againin 1985. The purpose initially was to measure attitudinal supportfor the then precariously placed and often criticized commissions.The follow-up survey was intended to measure whether attitudinalsupport had become greater or weaker after well-publicized Reaganadministration initiatives had led to reduced federal funding.The data show mixed findings in the two states but indicatethat, at least in some rural areas, greater acceptance of regionalismwas achieved in the face of decreased activities by regionalcommissions.