Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

In this report, we applied the TissueFAXS 200 digital pathological analysis system to rapidly and accurately identify neutrophils during regeneration of contused skeletal muscle, and to provide information for follow-up studies on neutrophils to estimate wound age. Rat injury model was established, and skeletal muscle samples were obtained from the control group and contusion groups at 1, 1.5, 2, 3, 4, and 6 h, as well as at 1, 3, 5, and 15 d post-injury (n = 5 per group). The expression of nuclei and neutrophils was detected by hematoxylin and eosin (HE) staining and immunohistochemical (IHC) staining. A total of 20 injury site areas of 0.25 mm² (0.5 mm × 0.5 mm) were then randomly selected at all time points. A TissueFAXS 200 digital pathological analysis system was used to identify the positive and negative numbers. Knowledge of five professional medical workers were considered the gold standard to measure the false positive rate (FPR), false negative rate (FNR), sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic (ROC) curves. As a result, with a staining area of neutrophils from 8 µm² to 15 µm², the FPR was 4.28%–12.14%, the FNR was 12.42%–64.08%, the sensitivity was 35.92%–87.58%, the specificity was 87.86%–95.72%, the Youden index was 0.316–0.754, the accuracy was 82.80%–88.30%, and the AUC was 0.771–0.826. The AUC was largest when the cut-off value of the staining area was 12 µm². Our results show that this software-based method is more accurate than the human eye in evaluating neutrophil infiltration. Based on the sensitivity and specificity, neutrophils can be accurately identified during regeneration of contused skeletal muscle. The TissueFAXS 200 digital pathological analysis system can also be used to optimize conditions for different cell types under various injury conditions to determine the optimal cut-off value of the staining area and provide optimal conditions for further study. Furthermore, it will provide evidence for forensic pathology cases.     

美国高校学生事务管理专业硕士生培养模式初探

近三十年来,美国高校学生事务管理专业的学科建设与人才培养在快速发展。该专业的硕士学位课程设置科学、研究生培养各环节的设计优化、实践性课程的特点突出,对当前我国高校学生事务管理专业的建立与完善有很好的借鉴意义。     

美国大学校长遴选与考核指标体系构建及价值借鉴

美国大学董事会是美国大学校长遴选与考核的主要机构。经董事会委托或授权而成立的校长遴选与考核委员会,无论是遴选校长还是考核校长,都普遍重视校长在学术管理、交流、筹款、判断、决策、合作能力和处理内外部关系等方面的能力。这些举措对于中国建立符合高等教育自身发展逻辑的大学校长遴选和考核制度具有一定的借鉴价值。     

基于信任视角的高校教师组织承诺的培育

高校教师是高等学校教学与科研的主体,是推动学校全面、协调、可持续发展的重要力量,高校教师组织承诺的培育是高校人力资源管理的关键.高校教师组织承诺的培育分为三个阶段:建立认知型信任--产生组织承诺;产生情感型信任一发展组织承诺;强化情感型信任一提升组织承诺.     

中国实施反贫困战略的法学分析

本文在分析我国贫困形成的制度性原因基础上 ,剖析了公共权力在反贫困中的作用 ,探讨了开发性扶贫中的基础资源配置问题、分配正义与反贫困的关系问题、贫困者的权利保障机制问题 ,并提出了我国制定《反贫困法》的构想。     

论警力开发

警力开发是使警察的社会治安控制能力得以提高和增强的过程。本文通过对目前公安队伍警力不强的原因及进行警力开发必要性的探讨,在此基础上阐述了如何开发警力的建议。     

TD-RAPD技术用于罂粟品种鉴定初探

目的探索TD-RAPD技术用于罂粟品种鉴定的可行性。方法采用改良CTAB法从罂粟叶片中提取DNA;TD-RAPD技术对种植于云南西双版纳地区的1个罂粟样品进行扩增分析。结果建立了罂粟DNA提取方法,从10个随机引物中筛选出6个引物用于罂粟TD-RAPD分析。结论TD-RAPD技术可用于罂粟DNA的分子标记,为罂粟DNA数据库建立提供技术方法,最终从DNA分子水平上追溯罂粟植物毒源。     

法院证据调查与当事人主义

我国审判方式改革在批判超职权主义观念的基础上,主张向当事人主义的目标发展,大大削弱了法官依据职权调查证据的权力。本文通过对大陆法系和英美法系的当事人主义诉讼模式的分析发现,对真实的追求是民事诉讼的真谛,当事人主义并不排斥法院实施证据调查。我国应当通过法院证据调查制度的规范化促进司法者能动地输出司法正义。     

新农民阶层与乡村司法理论的反证

作为当前学界主流的乡村司法理论,基于对乡村社会结构内向、封闭的理解,具有明显的地方化特征。然而,新农民阶层的出现及其推动的乡村社会变迁,导致乡村司法理论产生一些悖论性事实。这种变迁和悖论能够从"地位获得理论"与乡村司法运作存在的关联中加以解释。据此,现有的乡村司法理论应当有所修正,使得带有"治理"味道的地方化特征不再那么显著,转而复归到追求普适化的乡村法治化轨道。     

Validation studies of the ParaDNA® Intelligence System with artificial evidence items

Short tandem repeat (STR) profiling is one of the mostly used systems for forensic applications. In certain circumstances, STR profiling is time-consuming and costly, which potentially leads to delays in criminal investigations. LGC (Laboratory of the Government Chemist, UK) Forensics has developed a robust STR profiling platform called the ParaDNA^® Intelligence Test System which can provide early tactical intelligence and aid investigators in making informed decisions on sample prioritization for detection. Here, we validated the ParaDNA intelligence test for its application in forensic cases using a range of mock evidence items following guidelines set by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Specifically, we tested the sensitivity and accuracy of the ParaDNA intelligence test, as well as the success rates for detecting mock samples and for use in case scenarios. Our findings demonstrate that the ParaDNA intelligence test generates useful DNA profiles, especially for samples such as blood, saliva, and semen that contain ample DNA, indicating the benefits of including ParaDNA as a prior step in forensic STR profiling pipelines.