快速PCR扩增体系的建立及法医学应用

目的建立快速PCR扩增体系,探讨其在法医实践中的应用价值。方法设置不同的缓冲液、酶浓度与Mg SO4浓度、热循环参数等,观察不同参数对扩增结果的影响,建立快速PCR扩增体系。使用该体系对常见检材进行STR分型,与常规方法扩增的结果比较,考察其分型准确性与扩增用时。结果本文建立的快速PCR扩增体系含DNA TyperTM19试剂盒的Primer Mix(5×)2μL;Ta KaRa试剂的Fast BufferⅡ(10×)1μL,Speed STARTMHS DNA Polymerase(5U/μL)0.3μL,Mg SO4(50mmol/L)为0.1μL。扩增程序为95℃120s;95℃2s、61℃15s,27个循环;72℃60s。结论快速PCR扩增体系分型结果准确,用时仅19min,缩短了常规扩增用时,有较大的法医学应用价值。     

东盟产业集群发展的现状与特点

随着东盟工业化进程的加速发展,东盟各国产业集群逐步形成与发展,其比较优势和竞争优势也逐步显现。东盟较有竞争力的产业集群都有跨国公司的参与,跨国公司的投资带动了产业集群的发展,而产业集群又吸引了更多跨国公司的投资。跨国公司与东盟产业集群的互动,促进了各国的经济发展、产业调整和技术进步。     

Restructuring China's State Corporatist Industrial Relations System: the Wenling experience

Intensifying labour conflict in China has posed a serious challenge to the industrial relations system. Under growing pressure, the Chinese government has sought to reform the system but the results are meagre. Among the supposedly successful cases, the development of collective bargaining in Wenling, Zhejiang province has been hailed as a model of labour relations to be replicated elsewhere. Based on a detailed case study of Wenling, this study aims to analyze the process whereby local government reconstructs the industrial relations system by organizing and incorporating the interest of employers and workers, leading to regularized wage growth and reduced labour dispute. This restructuring, the study argues, is designed to create a functional state corporatist system by means of expanding union representation and instituting tripartite collective bargaining. However, the tensions in the state corporatist structure may still undermine any attempt by the government to reconstruct industrial relations.     

建构主义理论在痕迹检验课教学改革中的应用

建构主义学习理论,强调个体认识的能动作用,即认识并非是人脑对客观世界的直接简单反映,而是以自己原有知识为基础,通过指向事物的活动在各主体相互作用中建构而成的。也就是说,学习者必须亲自且主动进行知识的建构才能获得真正意义上的知识,否则不能。这一理论对公安院校痕迹检验课教学的改革,尤其是对当前形势下培养创新型公安人才所必须的创新性教学改革极具启发意义。     

论要约收购

要约收购是我国上市公司收购的重要方式。我国的法律对要约收购作了一些规定 ,但存在许多缺陷 ,需要进一步完善。重建强制要约收购 ,设立自愿要约收购 ,规制收购后续行为 ,是健全要约收购的重要步骤。     

Emergence of the Greater China Circle Economies: Cooperation versus competition

The integration of the so-called greater China economies among Mainland China, Taiwan, and Hong Kong is one part of the global trend of regionalism. The significance of integration in terms of foreign trade and foreign direct investment demonstrates not only rapid growth but also diversity, ever since Mainland China pursued her open door policy of economic reform in 1979. For instance, the combined volume of Hong Kong and Taiwan accounted for as much as 74.1% of Mainland China's capital utilization in 1993, which in turn contributed to the rapid economic growth of Mainland China over the last two decades. The membership of Mainland China and Taiwan to the WTO, as expected by the end of 2001, will facilitate a more official arrangement of intergovernmental coordination within these Triangle Economies. However, the government of Taiwan will continue to evaluate the cross-strait relations in the context of the nation's overall political and economic security as long as the government of Mainland China does not renounce the use of military force against Taiwan.     

保险诈骗犯罪客体的探讨

保险诈骗罪的直接客体是保险制度或保险秩序,具体而言,是保险合同关系或保险利益.保险诈骗罪的客体是简单客体,不是复杂客体.其犯罪对象是保险金.保险诈骗罪的同类客体是社会主义市场经济秩序.犯罪客体是复杂客体还是简单客体,关键是看犯罪构成要件的行为所侵害的对象反映出什么社会关系或社会利益.     

关于西部大开发战略若干问题的思考

西部大开发的战略正在紧锣密鼓的实施。如何保证西部大开发能够顺利、持续的进行 ,这是全国人民十分关注的事情。本文对西部大开发中的东西部关系、西部地区的自力更生与对外开发、物质文明与精神文明建设的关系 ,经济发展与环境保护的关系以及西部开发的过程等问题进行了一番思考。     

公共安全问题及其化解途径

公共安全问题包括自然灾害、治安事故、食品卫生、公共卫生、群体性事件、违法犯罪等多个方面。当前我国公共安全问题形势非常严峻,而和谐社会的目标之一就是稳定,严峻的公共安全问题与我国建设社会主义和谐社会的目标是相悖的。要化解公共安全问题需要健全和完善相关法律法规,建立国家公共安全综合管理体系和保障体系,提高应对公共安全问题的能力。