13C4-secobarbital as the internal standard for the quantitative determination of secobarbital--a critical evaluation

In this study, 13C4-secobarbital was used as an exemplar compound to illustrate the mechanism based on which the effectiveness of a proposed internal standard (IS) could be evaluated. A deuterated analog, 2H5-secobarbital, was also studied in parallel for comparison purposes. Well-established solid-phase extraction and methylation procedures were used prior to the GC/MS measurement step. The contribution of the intensity of an ion designated for the analyte (secobarbital) by the proposed IS, and similarly, the contribution of the intensity of an ion designated for the IS by the analyte--a phenomenon termed "cross-contribution"-were evaluated based on a "direct measurement" procedure in which equimolar amounts of the analyte and the IS were used to generate intensity data. These intensity data were then used as the basis for the calculation of "cross-contribution" (in percentages) of ions designated for the analyte and the IS. Cross-contribution data were compared with the linearity data resulting from two series of standards containing 25 to 9600 ng/mL secobarbital using two sets of quantitation ion pairs--m/z 196/200 and 195/199 with 13C4-secobarbital as the IS and m/z 196/201 and 195/200 with 2HS-secobarbital as the IS. 13C4-secobarbital was found to be much less problematic and thus can serve as a very effective IS. Cross-contribution data alone cannot fully explain the observed differences resulting from the use of these two ISs; further systematic study is needed to provide better understanding of the underlying interference mechanism.     

D1S80等位点在法医物证鉴定中应用价值评估

比较D1S80 ,DQA1+PM和CTT等位点在法医物证鉴定中应用情况 ,经研究发现 ,D1S80位点识别能力较强 ,基因遗传稳定 ,其电泳检测操作简单 ,易推广 ;DQA1+PM位点扩增片段较短 ,反向斑点杂交法灵敏度高 ,特别适用于陈旧、降解和微量生物检材的鉴定 ;CTT位点的荧光检测法稳定、可靠 ,实验结果便于保存和管理。总之 ,D1S80、DQA1+PM和CTT位点的遗传符合孟德尔定律 ,分型简便、可靠 ,在亲子鉴定和个人识别中发挥重要作用。     

用反相杂交技术对HLA-DQA1基因分型及其应用

目的 :HLA -DQA1其因的分型研究及在实际办案中的应用。方法 :PCR结合反相杂交检测技术对HLA -DQA1基因进行分型。结果 :检见7种基因 ,24种基因型 ,获得上海地区HLA -DQA1基因的基因频率及基因型的频率分布数据。结论 :对法医学中常见的血液 (痕 )、牙齿、精斑、混合斑、人体组织等检材进行HLA -DQA1分型检测 ,其结果稳定可靠 ,为实际办案提供了科学依据     

STR复合扩增及荧光检测技术在个体识别中的应用

目的 :使用 310型遗传分析仪对D3S1358等 10个位点进行基因型检测并应用于法医物证学个体识别案件。方法 :用PCR复合扩增结合四色荧光检测技术对样本DNA进行基因分型。结果 :常见物证检材可成功地得到检验。结论 :这些位点适用于法医物证学个体识别。     

Mind your manners: quality of manner of death certification among medical examiners and coroners in Taiwan

To assess the quality of manner of death (MOD) certification among medical examiners/coroners (ME/Cs) in Taiwan, death certificates issued in 2002 for which the final MOD was suicide or undetermined were extracted for analysis. Indicators of the quality of MOD certification included (1) MOD not given by the ME/Cs; (2) MOD assigned by the ME/Cs was changed by the coder; (3) ratio between undetermined and suicide deaths (U/S ratio). There were 450 death certificates for which the ME/Cs did not assign the MOD in the original certificate. Three fifths (285/450) of them were issued by 4 ME/Cs. The same 4 ME/Cs also had extremely high U/S ratios (1.25-1.84) compared with the average (0.31). The overall quality of MOD certification among ME/Cs in Taiwan was fair; only a small number of ME/Cs had poor quality in MOD certification. The high U/S ratio among the 4 ME/Cs would certainly affect the suicide mortality rates of the counties the 4 ME/Cs were in charge of. Actions should be taken to improve the certification quality of these 4 ME/Cs.     

放射影像学在颈部损伤尸体检验中的应用

多层面计算机断层成像(MSCT)和磁共振成像(MRI)对尸体骨骼系统和软组织损伤的诊断价值等同于传统的尸体解剖,运用在颈部损伤的尸体检验中可以弥补传统尸体解剖的某些不足,在某些特定的环境、腐败、烧焦或具有传染性的尸体检验中,MSCT和MRI联合使用可以作为机械性窒息、挥鞭样损伤、颈椎损伤及颈部血管损伤的可选择的法医学检查手段之一。     

广告文化中"自然"的意识形态化

"自然"这个原本作为自为存在的事物在广告文化中成了被大量操纵的能指符号用来推销产品,这同时也是重新塑造一个"自然"的过程,"烹调自然"与"回归自然"成了广告文化中两个重要的策略,而这种广告文化最终在改变着自然与人类之间的关系.这种广告文化对于"自然"与人类的关系影响巨大.     

关于珠海工商应对农村市场体系的对策与路径

农村市场是社会主义市场经济体系的有机组成部分,是推动或制约"三农"发展的重要力量.通过对珠海农村市场体系的历史回顾和现状阐述,在分析珠海农村市场体系的缺陷与不足的基础上,探讨在社会主义新农村建设的新的历史背景下,珠海工商行政管理部门关于建立和完善农村市场体系实践活动中的职能定位、应对策略和操作路径.