提取CO Ⅰ基因片段鉴定嗜尸性蝇类

目的通过扩增蝇类COⅠ基因片段,结合形态学特征鉴定嗜尸性蝇类的种类。方法运用改良平衡酚—tris饱和酚法提取蝇类DNA,进行COⅠ序列扩增和测序,与数据库序列进行比对和分析。结果改良平衡酚—tris饱和酚提取DNA方法可获得有效的蝇类DNA,并可应用于COⅠ基因片段扩增,进而进行蝇类种类的鉴定。结论平衡酚—Tris饱和酚法提取的噬尸性蝇类虫体DNA作为模板,用于COⅠ序列扩增,测序后与数据库目的序列分析比对,可准确鉴定嗜尸性蝇类的种类;和传统的昆虫形态学特征鉴定方法比较,该体系更准确,应用范围更广。     

脑钠尿肽的表达调控机制及其法医学应用

脑钠尿肽是主要由心肌细胞分泌的钠尿肽类激素,通常在心肌细胞受到牵拉时产生,具有利钠、利尿、舒张血管、调节血压等功能。临床上BNP是反映心功能的重要指标之一,近年的研究表明,BNP在法医学中对心源性猝死的诊断也具有十分重要的价值。本文综述了BNP的分子结构、表达调控及法医学应用的进展。     

“杀亲案件”135例法医学回顾分析

目的通过总结我省135例杀亲案件的案件资料,研究杀亲案件的特点,分析杀亲案件的侦办要点。方法收集2011~2013年间我国中部某省区域内发生的135例杀亲案件,对数据资料进行分析。结果杀亲案件主要发生在夫妻之间及父母子女,好发于农村,青壮年多见。一般无明显杀人动机,作案工具多为就地取材,死亡原因以机械性损伤和机械性窒息为主。结论杀亲案件在嫌疑人社会学特点、致伤工具、死亡原因、案发时间等方面反映出一定共性,但案件证据的收集是案件的侦办难点。     

To be committed or not: examining effects of personal and workplace variables on the organizational commitment of Southern prison staff

This study examined how personal and workplace variables were related to organizational commitment among staff working at a large Southern prison. The personal variables were gender, age, position, tenure, educational level, and supervisory status. The workplace variables were assessment of training, job variety, role clarity, input into decision-making, and instrumental communication. The results indicate workplace variables play a greater role in shaping affective commitment of surveyed Southern prison staff than do personal variables. The personal variables explained only 10% of the variance in the commitment index, while workplace factors accounted for approximately 59% of the variance and were significant determinants of organizational commitment among the respondents. In the multivariate regression analysis, age, assessment of training, job variety, role clarity, input into decision-making, and instrumental communication all had positive associations with commitment. Educational level had a negative relationship with commitment. Implications of these findings for policy and future research are also discussed.     

GENERAL AND PARTICULAR APPLYING ELEMENTS OF THE PRECAUTIONARY PRINCIPLE IN INTERNATIONAL TRADE

In the context of WTO dispute settlement, the precautionary principle is a predominant concept, in the face of serious risks and scientific uncertainty, that is often characterized as “better safe than sorry.” Although the precautionary principle appears in various treaties, declarations, and even laws, it is often formulated in abstract terms, leading to the great ambiguity of its primary elements. The Panel and the Appellate Body always avoid discussing the precautionary principle in WTO cases due to its obscure definition. On March 13, 2012, the United States, the European Union, and Japan each requested consultations with China as China’s measures that made restrictions on the exportation of rare earths, tungsten, and molybdenum had been in conflict with their national interest. After examining the matter at issue, the Panel and the Appellate Body rejected China’s arguments regardless of its repeated claims about its consideration of environmental protection. With the case as the starting point, this article focuses on the precautionary principle and explores its status and elements, in order to establish a two-dimensional model of the precautionary principle that is applicable in international trade.     

浅谈社区矫正若干问题——兼论人民法院在社区矫正中的作用

社区矫正在全国部分省、市的开展,标志着我国刑罚改革的重要进步。但在实践中,由于法律规定缺失或者过于粗疏,已越来越困扰社区矫正的正常开展,随着试点时间的增长,如果没有明确的法律支持,社区矫正工作将面临更大的困难。本文对社区矫正的部分立法问题及人民法院在社区矫正中的作用进行探讨,以期对推动这项工作有所裨益。     

An estimate of the proportion of drug-facilitation of sexual assault in four U.S. localities

In recent years, drugs including flunitrazepam, gamma-hydroxybutyrate, ketamine, and ethanol, have become popularly associated with drug-facilitated sexual assault. Other drugs are also candidates as factors in "drug facilitated sexual assault" (DFSA). The true extent of DFSA is not known, and is difficult to estimate. We recruited sexual assault complainants at four clinics in different parts of the U.S. to anonymously provide urine and hair specimens, and to answer questions about suspected drugging, drug use, and the sexual assault incident. Urine and hair specimens were tested for 45 drugs, including ethanol, and those pharmacologically capable of inducing sedation, amnesia, or impairment of judgment. Analytical test results were used to estimate the proportion of subjects, and the proportion of all complainants to the clinic in the same time period, who were victims of DFSA. Overall, cases of 43% of 144 subjects, and 7% of 859 complainants, were characterized as DFSA. Subjects underreported their use of drugs. The role of toxicological results and history in characterizing DFSA cases is discussed.     

Guidelines for postmortem protocol for ocular investigation of sudden unexplained infant death and suspected physical child abuse

Postmortem examination is a cornerstone in identifying the cause of unexplained sudden death in children. Even in cases of suspected or known abuse, an autopsy may help characterize the nature of the abuse, which is particularly important in the forensic autopsy of children in the first 3 to 4 years of life when inflicted neurotrauma is most common. Forensic examinations are vital in cases that might otherwise be diagnosed as sudden infant death syndrome. The ocular autopsy in particular may demonstrate findings that were not appreciated on antemortem clinical examination. This protocol for postmortem examination of the eyes and orbits was developed to promote more consistent documentation of findings, improved clinical and forensic decision making, and more replicable and coherent research outcomes.     

Sudden cardiac death due to giant cell inflammatory processes

Granulomatous inflammation of the myocardium may occur in a number of systemic disease processes including those with infectious etiologies such as fungal, mycobacterial and parasitic infections, as well as hypersensitivity reactions, and rarely autoimmune disorders. In many of these disorders, giant cells are components of the inflammatory infiltrate. Systemic granulomatous processes of unknown pathogenesis, most notably sarcoidosis, may also be associated with involvement of the myocardium. Occasionally, these disorders are associated with sudden death due to pathologic involvement of the heart. In contrast, giant cell myocarditis, also known as idiopathic myocarditis, a rare, frequently fulminant and fatal disorder of unknown etiology, is isolated to the heart and lacks systemic involvement. This disorder is most commonly diagnosed at autopsy. We present two cases in which sudden death resulted from a giant cell inflammatory process affecting the myocardium. Both individuals lacked antemortem diagnoses and collapsed at their respective places of employment. These cases compare and contrast the clinical and pathologic issues involved in the differential diagnoses of the subgroup of sudden cardiac deaths resulting from giant cell inflammatory processes that affect the myocardium, as well as the value of histologic examination and immunohistochemical studies.