Estimating Individual Contributions to Complex DNA SNP Mixtures.,

High‐throughput sequencing (HTS) of large panels of single nucleotide polymorphisms (SNPs) provides an alternative or complimentary approach to short tandem repeats (STRs) panels for the analysis of complex DNA mixture forensic samples. For STRs, methods to estimate individual contribution concentrations compare capillary electrophoresis peak heights, peak areas, or HTS allele read counts within a mixture. This article introduces three approaches (mean, median, and slope methods) for estimating individual DNA contributions to forensic mixtures for HTS/massively parallel sequencing (MPS) SNP panels. For SNPs, the major:minor allele ratios or counts, unique to each contributor, were compared to estimate contributor proportion within the mixture using the mean, median, and slope intercept for these alleles. The estimates for these three methods were typically within 5% of planned experimental contributions for defined mixtures.     

No Handmaidens Here: women,volunteering and gender dynamics in the Sydney New Theatre

This paper considers the role of women in the Sydney branch of the New Theatre, from 1936 to 1969. In contrast to other gendered spaces found in the theatrical, industrial and political spheres, women held together the New Theatre. Not only did the theatre give opportunities to women as performers, but women embraced roles as directors, stage managers, writers, designers as well as holding elected offices. Drawing on oral histories and archival research, this study presents new scholarship on Australian women’s leadership in the theatre, arguing that their pattern of involvement was shaped by the voluntary nature of the work, the longevity of involvement, their political commitment and the theatre’s democratic structure. The blending of organisational and creative leadership created spaces for women’s voices in ways that were crucial to the long-term success of the Theatre, at a time when women were generally expected to focus on the domestic sphere.     

Hearts of Darkness: white women write race

Abstract

In an effort to popularise the gas stove, boost sales and minimize the genuine and perceived dangers of using this volatile domestic appliance, late Victorian gas managers and stove manufacturers employed female cookery teachers to perform public demonstrations at trade exhibitions throughout the country. Eventually, these women comprised a special department of gas sales, calling directly on customers in their homes to offer personal instruction in the proper use and care of gas stoves. Part social workers, part salesgirls, the ‘lady demons’, short for lady demonstrators, moved between the traditionally gendered separate spheres of work and home, consciously constructing a professional image that reconciled the social tensions of this new occupation. They offer an early example of women's entrance into the corporate business world, the feminisation of consumption, and the combination of customer service with social welfare.     

Confrontation and co‐operation in the Indian Ocean

The end of the Cold War has created new dangers and opportunities. The formation of an Indian Ocean Rim trading bloc has the potential to benefit some of the poorest people in the world. Regional maritime co‐operation could provide the catalyst for alleviating poverty on the Indian Ocean Rim by giving a spurt to incomes and employment from the seas.