Using FastID to analyze complex SNP mixtures from indoor dust

Forensically relevant single nucleotide polymorphisms (SNPs) can provide valuable supplemental information to short tandem repeats (STRs) for investigative leads, and genotyping can now be streamlined using massively parallel sequencing (MPS). Dust is an attractive evidence source, as it accumulates on undisturbed surfaces, often is overlooked by perpetrators, and contains sufficient human DNA for analysis. To assess whether SNPs genotyped from indoor dust using MPS could be used to detect known household occupants, 13 households were recruited and provided buccal samples from each occupant and dust from five predefined indoor locations. Thermo Fisher Scientific Precision ID Identity and Ancestry Panels were utilized for SNP genotyping, and sequencing was completed using Illumina^® chemistry. FastID, a software developed to permit mixture analysis and identity searching, was used to assess whether known occupants could be detected from associated household dust samples. A modified “subtraction” method was also used in FastID to estimate the percentage of alleles in each dust sample contributed by known and unknown occupants. On average, 72% of autosomal SNPs were recovered from dust samples. When using FastID, (a) 93% of known occupants were detected in at least one indoor dust sample and could not be excluded as contributors to the mixture, and (b) non-contributor alleles were detected in 54% of dust samples (29 ± 11 alleles per dust sample). Overall, this study highlights the potential of analyzing human DNA present in indoor dust to detect known household occupants, which could be valuable for investigative leads.     

Terrorist Victimization: Prevention,Control, and Recovery

The Muslim Brotherhood poses a unique challenge to efforts to combat Al Qaeda and like-minded groups. It is one of the key sources of Islamist thought and political activism, and plays a significant role in shaping the political and cultural environment in an Islamist direction. At the same time, it opposes Al Qaeda for ideological, organizational, and political reasons and represents one of the major challenges to the salafi-jihadist movement globally. This dual nature of the Muslim Brotherhood has long posed a difficult challenge to efforts to combat violent extremism. Does its non-violent Islamism represent a solution, by capturing Islamists within a relatively moderate organization and stopping their further radicalization (a “firewall”), or is it part of the problem, a “conveyor belt” towards extremism? This article surveys the differences between the two approaches, including their views of an Islamic state, democracy, violence, and takfir, and the significant escalation of those tensions in recent years. It concludes that the MB should be allowed to wage its battles against extremist challengers, but should not be misunderstood as a liberal organization or supported in a short-term convergence of interests.     

Escherichia coli,Corporate Discipline and the Failure of the Sewer State

Recent panics over adulterated beef have led the US federal government to institute what it calls a ‘risk-based, science-based’ approach to food safety. Rather than having federal inspectors monitoring carcasses for microbial contamination, the USDA has implemented a system known as HACCP that asks firms to regulate their own production processes. HACCP is an incidence of audit, a new method of governmentality widely used by state agencies. This paper argues that although ‘sewer states’ try to create trust in government by eliminating contamination, audit-based systems paradoxically create anxiety by highlighting overflows of filth and ‘zones of wildness’ where contamination is rampant.     

Deaths Involving Methylenedioxypyrovalerone (MDPV) in Upper East Tennessee

Two deaths involving 3, 4‐methylenedioxypyrovalerone (MDPV) are reported. MDPV is a synthetic cathinone stimulant found in “bath salts” with neurological and cardiovascular toxicity. Biological specimens were analyzed for MDPV by GC/MS and LC/MS. A White man was found dead with signs of nausea and vomiting after repeatedly abusing bath salts during a weekend binge. Femoral venous blood and urine had MDPV concentrations of 39 ng/mL and 760 ng/mL. The second fatality was a White man with a history of drug and bath salt abuse found dead at a scene in total disarray after exhibiting fits of anger and psychotic behavior. Femoral venous blood and urine had MDPV concentrations of 130 ng/mL and 3800 ng/mL. The blood and urine MDPV concentrations are within the reported recreational concentration ranges (blood 24–241 ng/mL and urine 34–3900 ng/mL). Both decedents’ deaths were attributed to relevant natural causes in a setting of MDPV abuse.     

A novel FTA™ elute card collection method that improves direct DNA amplification from bloodstained concrete

Concrete is a common construction material found in residential and commercial buildings, bridges and parking lots that is a composite matrix containing aggregate held together with cement. The porous nature of concrete can make the collection and genotyping of biological fluids, such as blood, challenging. Forensic evidence can become embedded within the matrix, potentially reducing the amount of DNA available for analysis. In forensic science, “direct” amplification refers to a genotyping method that amplifies a DNA profile directly from a sample without DNA extraction, saving time and money. We investigated a novel application of Whatman? FTA? Elute cards in their ability to directly amplify PowerPlex® Fusion and Y23 profiles from minute amounts of blood that had been deposited on different concrete structures. In comparison to traditional collection methods, directly profiling blood stained construction materials using FTA? Elute cards increased the percentage loci amplified and significantly improved both allele peak height and peak height ratio while reducing allelic drop-out. FTA? Elute cards can provide a reliable, inexpensive and superior alternative to traditional methods.     

Accuracy and Reliability of Total Body Mass Estimation Techniques from Stature and Bi‐iliac Breadth in Non‐Hispanic U.S. Whites from the Bass Donated Skeletal Collection

This paper tests the fidelity of a recent method that used the NHANES III dataset as a proxy to estimate total body mass from stature and bi‐iliac breadth in U.S. White males and females. The bi‐iliac breadths of 230 males and 152 females identified as non‐Hispanic U.S. White from the Bass Donated Skeletal Collection were measured, and along with stature from predonor paperwork, total body mass estimates were calculated and then compared to body masses recorded on predonor paperwork. Male and female samples were subdivided by body mass index (BMI [kg/m²]) categories established by the World Health Organization. Our results suggest that total body mass estimates can be accurately assessed provided that the individual is within 18.50 ≤ BMI ≤ 29.99 for White males and 18.50 ≤ BMI ≤ 24.99 for White females. Recommendations on how to report total body mass estimates are also presented.     

The formation of in-formation by the US military: articulation and enactment of infomanic threat imaginaries on the immaterial battlefield of perception

This article explores the role of perception management as a doctrinal concept in the construction and deconstruction of threats. Threat construction involves a continual, reciprocal and constitutive relationship between the attribution of meaning and practice. It takes place in an environment changed by the multiple forces of the so-called information revolution, in which discursive links are forged between technology and victory as well as between scientific progress and military superiority. Through articulation and enactment, information and information technology are represented as both threatening and threatened in three dimensions: dependency on information as data, possible loss of information superiority, and loss of control over information as image. The enemy ‘Other’ in these threat constructions is everywhere and nowhere at the same time, and is seen as being greatly empowered by the same tools that empower the United States military establishment. But while, for many military actors, regaining control in the information domain through technological innovation seems more or less straightforward, the immaterial battlefield of perception proves harder to master. We purport that military strategists do not seem to fully grasp the illusionary nature of the belief that the information space is a thing to be shaped at will and without restrictions.     

On the Prospects of a Return to Keynes: Taking Keynes’s Political Philosophy Seriously

This article addresses the prospects of a “return to Keynes” in terms of Keynes's own philosophy. It shows that Keynes's moral and political philosophy provide little guide to how Keynesian economics might now be achieved. Keynes's gradualist reformism, derived from both Burke and Moore, leaves a gulf between his economic agenda and the means of its implementation, which is widened in attempts to transpose his proposals onto the global political economy of the 21st century. Keynes's faith in elite intuition and enlightened rule are never securely established and are undermined by his own insights into uncertainty. However, the priority of the short-run and Keynes's depictions of organic unity suggest potential if underdeveloped avenues for alternative social choices and policy re-direction.     

Changing the design: cabinet decision-making in three provincial governments

Abstract: The subject of this investigation is cabinet decision-making in the provincial governments of Saskatchewan, Manitoba, and British Columbia. Specifically, it attempts to examine the forces that underlie the initiation and persistence of cabinet institutionalization in the postwar period. The postwar period has witnessed the replacement of the unaided (or traditional) cabinet by the institutionalized (or structured) cabinet. In other words, unstructured and relatively uncoordinated central executives have given way to those which are more structured, more collegial, and more prone to emphasize planning and coordination. The factors promoting initial cabinet institutionalization in the three provinces were a mixture of ideology, pragmatism, and historical precedent unique to each province. There were both endogenous factors (those growing from within government) and exogenous factors (those acting from without) which affected the persistence of institutionalized cabinets. They were common to more than one province but their relative weight differed between premiers. Not surprisingly, the institutionalization of provincial cabinets has had major effects on political actors and functions in the three provinces studied. Cabinet structure has both changed and been changed by power relations within cabinet. Full cabinet appears to have been overshadowed as a decision-making center. It is apparent that central agencies and central departments do not always yield similar political effects. Lastly, planning and budgeting have grown in both complexity and complementarily. Sommaire: Cette enquête traite du le processus décisionnel au sein du Conceal des ministers dans les governments provinciaux de la Saskatchewan, du Manitoba et de la Colombie-Britannique. Plus précisément, elle cherche B examiner les forces qui ont favorisé l'apparition puis le maintien de l'institutionnalisation des Conseils des ministres au cours de l'après-guerre.