Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides

Traditionally, sperms are isolated from vaginal cell mixtures by preferential extraction methods. Although these methods work well when there is a reasonable amount of DNA present, they are problematic when there are limited amounts (ca. 250 pg). In particular, the analysis of sperm from microscope slides has proven difficult. Here, we describe the use of laser capture microdissection (LM) for the isolation of spermatozoa from microscope slides containing sperms and vaginal cells. Such slides are frequently an important source of evidential material during the forensic investigation of rape and other sexual assaults. Low copy number (LCN) PCR was used to compare profiles of sperm DNA prepared using LM and preferential lysis. LM was found to outperform preferential lysis in 15 out of 16 samples. The application of LM to the processing of actual casework slides, and in particular the potential use of LM for the analysis of old cases, is discussed. Finally, 77 post-coital slides were processed in order to accurately assess the robustness of the technique. There was a significant association between the quality of the male profile recovered and time since intercourse that was independent of the number of sperms analysed, suggesting that the DNA was degraded even though the spermhead was intact.     

Protective Factors Interrupting the Continuity From School Bullying to Later Internalizing and Externalizing Problems: A Systematic Review of Prospective Longitudinal Studies

A systematic review is presented, based on prospective longitudinal studies, on protective factors that interrupt the continuity from bullying perpetration at school to externalizing problem behaviors later in life; and from bullying victimization to later internalizing problems. Some common factors were established, which seem to interrupt the continuity from school bullying perpetration and victimization to later maladjustment problems. These included having a good performance at school and good social skills, coming from a stable (undisrupted) family, being attached to parents, and having prosocial friends. Findings are consistent with the wider resilience literature on factors that confer emotional and behavioral adjustment to school youth. This is not surprising given the interconnectedness and common pathways between bullying and other risk behaviors. Effective antibullying and other multicomponent programs may successfully interrupt this long-term continuity. Future intervention research should be informed by the resilience literature and incorporate existing findings in future program planning.     

Characteristics of female patients admitted to an adolescent secure forensic psychiatric hospital

Bluebird House is the only mixed gender NHS secure forensic psychiatric hospital for adolescents in the South of England. It has admitted more than 30 female patients since the service opened in 2008. The admission criteria are that patients must be detained under the Mental Health Act and present evidence of being a risk of harm to others. This article describes the clinical characteristics of 30 consecutive female patients admitted to a highly specialised adolescent forensic inpatient service. Key results include a very high rate of incidents of risk behaviours exhibited by female patients within the unit but good clinical outcomes. The majority of patients had severe symptoms of mental disorder, especially emotional instability, self-harm behaviours and aggressive behaviours. Few had diagnoses of mental illness. Assessment findings from the Millon Adolescent Clinical Inventory and the Structured Assessment of Violence Risk are discussed, as are parallel with studies from other female secure services.     

How the ‘entry’ defines the ‘exit’: contradictions between the political and military strategies adopted in 2001 and how they have deleteriously affected the longer-term possibilities for stabilisation in Afghanistan

Focusing on the period 2001–2003, the paper discusses how the Western military intervention in Afghanistan changed power structures early on, undermining the political settlement in the long term and culminating in the present electoral crisis. Key concepts, a theoretical framework and the initial military approach (including how the initial military strategy undermined an indigenous solution for toppling the Taliban) are outlined and literature focused on hybrid governance and ‘legitimacy’ as an important but overlooked facet of peace-building is presented. Examples of ‘lessons learned’ in relation to the failure to engage with traditional forms of governance–early on in the Afghan conflict—are set out. The objective is to show that the military strategy adopted in 2001–2002 had adverse long-term consequences that were hard to reverse later on (e.g. by shifting the goalposts of military activity to, for example, a counter-insurgency strategy from 2005 onwards). This paper does not aim to resolve these paradoxes in the Afghan context going forward but offers implicit recommendations based on ‘lessons learned’.     

The Good Lives Model – new directions for preventative practice with children?

The study of young offenders has tended to focus on adolescents, despite knowledge that those who are engaging in criminality during childhood are more likely to experience long-lasting, life-impairing consequences. This qualitative study investigated how child offenders experience the process of desisting from crime. It was hoped that this would provide further insight for those involved with prevention programmes for young offenders. Seven young people aged between 10 and 18, engaging with the Preventing Youth Offending Project (PYOP) in the UK were interviewed, and the data collected was subject to Interpretative Phenomenological Analysis. Four themes emerged from the narratives, all converging on a changed self-identity for those successfully desisting. PYOP aims to enhance the lives of young people, and this approach appears to encourage this identity transformation through the provision of purposeful activity, supported education and mentoring. The increasing popularity of strengths-based enhancement approaches to rehabilitation, such as the Good Lives Model (GLM), is discussed in relation to its potential role in the prevention of criminality in young people. It is proposed that the GLM principles could provide essential foundations for early intervention approaches as well as rehabilitative measures for established offenders.     

The Effects Of Children'S Age and Delay on Recall in a Cognitive or Structured Interview

The study examined; (i) whether the enhanced cognitive interview (ECI) would aid event recall when used with children, (ii) whether the effects of a delay between the witnessed event and interview would have an impact on the effectiveness of the ECI, (iii) whether the age of the child would have a bearing on the effectiveness of the ECI, and (iv) which category(ies) of event recall might be effected. Thirty-two 8 to 9 year old children and thirty-two 11 to 12 year old children were shown a video recording of a staged shoplifting. Half were interviewed four hours after viewing the event and half after a six day delay. Children were interviewed individually using either the ECI or a structured interview (SI). Those interviewed using the ECI recalled significantly more correct details (especially detail pertaining to actions) with no increase in the reporting of erroneous information. The ECI was found to be a reliable interviewing technique regardless of age and delay.     

Deficits in Theory of Mind,empathic understanding and moral reasoning: a comparison between young offenders and non-offenders

Previous research suggests a lack of pro-social skills is characteristic of an antisocial or offending personality. It is therefore reasonable to assume that an inadequate understanding of another's mental state may contribute to antisocial or offending behaviour. Forty-six young-adult male offenders and a control completed measures to assess: Theory of Mind (ToM), empathic understanding (EU) and moral reasoning. Significant differences in the performance of young-adult offenders and the control group were detected in ToM, EU and moral reasoning with young-adult offenders scoring lower than the control group. A positive association was also found between ToM, EU and moral reasoning. These findings contribute to a further understanding of how individuals make sense of, and respond to, the social world around them. The ability to measure ToM, EU and moral reasoning and subsequently identify any specific deficits, as well as recognise the link between these three key skills, is not only useful for researchers but it will also allow practitioners to tailor existing (or develop new) interventions specific to the needs of an individual. This could be particularly useful in terms of recidivism when applied to those involved in antisocial or offending behaviour.     

Predicting dropout from a residential programme for adolescent sexual abusers using pre-treatment variables and implications for recidivism

Abstract

This study addresses the prediction that dropout from a UK specialized residential treatment program for adolescent sexual abusers can be determined from pre-treatment variables. Participants were 49 adolescents aged 12–16?years, who had sexually abused children, peers/adults or both. Of the variables examined, 25 showed a significant association with treatment dropout. A scale, consisting of 20 items, was designed to predict treatment dropout. As a measure it showed internal consistency (alpha?=?0.84) and predictive validity. Treatment dropout was linked to a greater risk of recidivism: offences of a general; violent; and combined violent or sexual nature. Missing data confounded the overall small sample size; therefore, a brief checklist of factors associated with dropout was produced as a guide for treatment managers and clinicians.     

Regionalism,personalism, ethnicity,and violence: parties and voter preference in the 2010 parliamentary election in Kyrgyzstan

On the basis of interviews, census data, and precinct-level electoral results from the October 2010 parliamentary election in Kyrgyzstan, this paper examines the influences on the party vote in Kyrgyzstan. Instead of traditional socioeconomic variables, this study finds that regionalism (North vs. South), ethnicity, personalism, and violence offered the most compelling explanations of party preference in Central Asia's most competitive election. The paper also examines the contextual factors that constructed and filtered voter demand, from Kyrgyzstan's political and economic conjuncture in 2010 to its unusual party system and electoral rules.