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171.
Abstract: A short tandem repeat multiplex assay has been successfully developed with 25 autosomal loci plus the sex‐typing locus amelogenin for a total of 26 amplified products in a single reaction. Primers for the loci were designed so that all of the amplicons present were distributed from 65 base pairs (bp) to less than 400 bp within a five‐dye chemistry design with the fifth dye reserved for the sizing standard. A multiplex design strategy was developed to overcome challenges encountered in creating this assay. The limits of the multiplex were tested, resulting in the successful amplification of a wide range of genomic DNA sample concentrations from 2 ng to as low as 100 pg with 30 cycles of PCR. The 26plex has the potential to benefit the forensic community for reference sample testing and complex relationship evaluation.  相似文献   
172.
Public procurement is a large sector of the economy with most procurement going to the defense sector. Procurement by the defense sector includes purchases made through contracts to private businesses that manufacture durable goods. Manufacturing of these goods results in pollution production with toxic wastes being among the most dangerous pollutants for public health. Despite green purchasing policy goals, most transactions in the United States through defense contracts result in disproportionately high-toxic pollution releases by manufacturers. We find that persistent exemptions granted defense agencies from following green purchasing policy result in a landscape where contractor environmental performance is unchanging with defense contractors persistently polluting in high amounts. Further, we find that defense contractors are linked to most toxic releases from procurement meaning that exemptions may be hindering potential advancements from green purchasing policy. Results can inform the design of new guidance about procurement and expand understanding of environmental inequality.  相似文献   
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Lisa Hill 《政治学》2001,21(2):101-113
This article responds to recent cases of parliamentary speech which reflect the ascendancy of a totalising 'mainstream' approach to public discourse and a political leadership that may, at times, be overly attentive to the majority-rule dimension of democracy. These developments spark a more general discussion of the phenomenology of privileged parliamentary speech, the role of speech freedoms in liberal democratic orders and the duties of parliamentary representatives within them. I make two general conclusions. First, the ways in which we normally argue and think about free speech will not generally apply to the speech of parliamentarians because their speech rights cannot be universalised. Secondly, even if parliamentary speech could be treated as standard speech there would be no legitimate defence (from a liberal democratic point of view) for a strictly populist approach to its use since this could undermine the deliberative function of parliament and lead to the violation of other important liberal democratic principles.  相似文献   
174.
This article examines the implications of high levels of informal (or invalid) voting in Australian national elections using a social exclusion framework. The rate of the informal vote is an indicator of social and political exclusion with particular groups of Australians experiencing inordinate electoral disadvantage. Poorer voters, voters from non‐English speaking backgrounds and those with low education levels are especially disadvantaged by factors peculiar to the Australian voting experience. We begin by exploring the character and pattern of informal voting and then canvass the technical and socio‐economic factors which explain it. We conclude by considering proposed options for reducing informality, some of which are: the abandonment of compulsory voting, major structural change to the voting system as well as ballot re‐design, electoral education and community information initiatives.  相似文献   
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This paper focuses on labour within immigration detention in the United Kingdom, offering an original national case study as well as a new conceptual framework for analysing such practices. It does so through an innovative engagement with recent literatures on forced labour, unfreedom and hyper-precarity, particularly amongst irregular migrants. We advance two key arguments in this paper. First, that the available data on labour within immigration detention indicate that detainees should legally be considered employees and granted access to labour protections, including the national minimum wage. Second, that work in immigration detention is an example of state-sanctioned exploitative, coercive and unfree labour amongst a hyper-precarious group of the population. This case has implications for other country contexts where immigration detention is used.  相似文献   
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