Primer binding site mutations affecting the typing of STR loci contained within the AMPFlSTR SGM Plus kit

The Forensic Science Service carries out human identification and familial investigations using the AMPFlSTR SGM Plus kit (PE Biosystems, Warrington, England). We have studied approximately 42,000 parent/child allelic transfers (meioses) for deviations from expected Mendelian Inheritance patterns. Of 55 apparent mutations detected, 20 had patterns suggestive of the presence of a primer binding site mutation producing a silent/null allele. The presence of a silent allele was unequivocally demonstrated in 13 of the 20 suspected cases by using alternative primer sets. Of the 13 confirmed cases, 9 involved the D18S51 locus. As the individuals in these cases all originated from the same geographic region of the Middle East, this cluster suggests the presence of a relatively common variant D18S51 allele in that particular group. These data taken together with our previously published work, confirm that the primer binding sites utilised for amplification of the loci contained in the AMPFlSTR SGM Plus kit have highly conserved nucleotide sequences.     

Amphetamines in washed hair of demonstrated users and workplace subjects

Cardiovascular complications of cocaine abuse include myocardial ischemia and infarction, dysrhythmias, cardiomyopathies and aortic dissection. The case in point pertains to a 26-year-old, Caucasian male, substance abuser who suffered a thoracic aortic dissection following the use of crack cocaine. The autopsy and histological findings showed a connective tissue abnormality including a focal microcystic medial necrosis and a fragmentation of the elastic fibers in the arterial walls. Blood concentrations of cocaine and benzoylecgonine, taken individually, were considered to be within a potentially toxic range. Blood concentrations of methadone also indicated use of this drug at the same time. The small amounts of morphine found in the blood and urine were compatible with heroine or morphine use more than 24 h before death.     

Incapacitation and fires

Traditionally quoted levels of the products of combustion are too high to account for all the problems of incapacitation and death in fires. Because people may not die instantly in a fire, they can continue to inhale toxic gases after collapse. Collapse may occur very quickly. Experience drawn from analysing the effects of the Manchester Boeing 737 accident and a building fire are used to debate this issue.     

An Investigation of Personality Traits in Relation to Adolescent School Absenteeism

We examined the Big Five personality traits of Agreeableness, Conscientiousness, Emotional Stability, Extraversion, and Openness, as well as four narrower traits of Aggression, Optimism, Tough-Mindedness, and Work Drive in relation to absences from school for middle- and high-school students. Participants were 248 seventh grade students, 321 tenth grade students, and 282 twelfth grade students. Most of the Big Five absence correlations were significant in the expected direction at all 3 grade levels. While Aggression, Optimism, Work Drive were significantly related to absences, only Work Drive added incremental variance to the prediction of absences beyond the Big Five traits. Study results were generally similar across grade levels. Findings were discussed in terms of dispositional absenteeism, the generalizability of the Big Five trait model, and the utility of more narrowband traits. Implications were drawn for early identification of absence-prone students and the precedent role of personality variables in school absence research on the effects of other variables, programs, and interventions.     

Municipal Bond Issuance: Is There Evidence of a Principal-Agent Problem?

Is there evidence of a principal-agent problem in the municipal bond industry? Do public managers, either on their own or through private sector agents, act to increase their own utility at the expense of the public? This article examines municipal bond decisions in the context of principal-agent theory based on data collected through a random sample survey of municipal bond issuers. Principal-agent theory is not well developed in the public sector. In the municipal bond industry, however, we have evidence that it helps explain the actions of public managers and elected officials, in particular for pay to play and the importance of interest rates vis-à-vis relationships. These actions carry potential costs to citizens.     

Menarcheal status and parent-child relations in families of seventh-grade girls

The associations between menarcheal status and several child-rearing and outcome variables were examined for mother-daughter and father-daughter dyads. All variables were assessed with questionnaires as an extension of earlier observational studies. Analyses were conducted via multiple regression analyses wherein menarcheal status was treated as a continuous variable and was entered into the regression equation as a set of power polynomial terms. The results indicated that most of the significant relations occurred for the mother-daughter dyad, and most of these relations were curvilinear. When menarche occurs at or around the modal time, changes in parent-child relations may be best thought of as temporary perturbations, but when menarche occurs early the effects may persist.The research reported here was funded by Father Flanagan's Boys Home, Inc., and by a grant to the senior author from the John D. and Catherine T. MacArthur Foundation, Family Relations in Early Adolescence.Received his Ph.D. in clinical psychology from Harvard University.Received his M.S. from Virginia Commonwealth University.Received her M.S. in counseling psychology from Virginia Commonwealth University.Received his M.S. from the University of Nebraska.She received her Ph.D. in developmental psychology from the Department of Human Development and Family Studies, Cornell University.     

Adolescents’ Experiences of Victimization: The Role of Attribution Style and Generalized Trust

Positive attribution style, negative attribution style, and generalized peer trust beliefs were examined as mediators in the relationship between adolescents’ peer victimization experiences and psychosocial and school adjustment. A total of 280 (150 female and 130 males, M_age = 13 years 4 months, SD_age = 1 year 1 month) adolescents completed measures of peer victimization, global self-worth, depressive symptoms, social confidence, school liking, loneliness, attribution styles, and generalized trust beliefs. Multigroup path analysis revealed that: (a) negative attribution style mediated the relationship between cyber victimization and school liking and depressive symptoms for males and females; (b) positive attribution style mediated the relationship between cyber victimization, school liking, global self-worth, and depressive symptoms for females; and (c) generalized peer trust beliefs mediated the relationship between social victimization, depressive symptoms, social confidence, and loneliness for females. Consequently, attribution style and generalized trust beliefs differentially influence the relationship between peer victimization and adjustment.     

On the Meaning of Survey Reports of Roll‐Call “Votes”

Contemporary efforts to evaluate representation often compare survey measures of how citizens say they would vote on legislation to what elected officials do in office. These comparisons generally suggest poor representation. We argue here that this common design is unlikely to effectively evaluate representation because responses to survey questions differ in important aspects from voting in legislatures. Measurement error and construct validity undermine the comparison. Three survey experiments show that providing partisan and nonpartisan information readily available to legislators materially changes respondents' expressed preferences on roll‐call votes. With information, expressed policy positions are both less centrist and more closely matched to legislator behavior in their preferred party. Respondents also appear aware of their own lack of knowledge in evaluating roll‐call policy votes. The treatment effect of information decreases in confidence judging policy in that area. We show similar patterns for respondent opinions on Supreme Court decisions.     

The single most polymorphic STR Locus: SE33 performance in U.S. populations

The STR locus SE33 (ACTBP2) located on chromosome 6 (6q14) is arguably the most polymorphic marker examined thus far by the forensic community with a heterozygosity of >0.95 in some populations. Three different primer sets were utilized in this study in order to assess the possibilities of primer binding site mutations. Population variation was measured in 460 U.S. Caucasian, 445 African American, 336 Hispanic, and 202 Asian samples along with mutation rates from almost 400 father–son pairs. In addition, the 10 genomic DNA components in NIST Standard Reference Material SRM 2391b were sequenced and found to exhibit a variety of additional base changes, insertions, and deletions outside of the SE33 repeat region.     

An Investigation of Biographical Information as a Predictor of Employment Termination among Law Enforcement Officers

An important service that police psychology can offer law enforcement administrators is assistance in the selection of future officers. Police psychologists have traditionally focused on the identification of psychopathology and exclusion of applicants who were found to be mentally ill. The introduction of the Americans with Disabilities Act (1990) has changed what had been considered appropriate exclusion criteria because of the introduction of increased civil rights protections for persons with disabilities. At the same time, increasing civil liability for law enforcement departments in regard to officer misconduct has compelled police psychologist to evaluate potential for future misconduct by non-clinical means. The scientific examination of biographical data may be one method of establishing the relationship between officer candidate variables and future misconduct. This paper examined the predictive validity of biographical information for a sample of 2965 police officer candidates. Results indicated that several biographical variables were significantly correlated with later termination. However, results of regression analyses indicated that biographical variables alone accounted for limited variance in the prediction of police officer termination. The use of biographical information as a sole determinant of police candidate suitability was not supported and the use of additional sources of data would be required for effectual forecasting purposes.