Determination of cocaine and benzoylecgonine in human amniotic fluid using high flow solid-phase extraction columns and HPLC.

A new solid-phase extraction procedure for the determination of cocaine and benzoylecgonine in amniotic fluid, using high flow co-polymeric sorbents is reported. The recoveries of cocaine and benzoylecgonine within the range 0.1-1 mg/l were 95.7% and 50.3%, respectively. The use of high-flow sorbents allowed the easy extraction of amniotic fluid regardless of sample viscosity or physical nature. The use of these solid-phase columns provided many advantages over the more commonly used solvent extraction, including an increase in extraction speed and efficiency, reduced operator time, reduced solvent use and disposal volumes and exceptional extract quality. Further, the determination of amniotic fluid obtained from pregnant cocaine users may provide important information about handling of cocaine by the fetus at various gestational ages. The procedure was successfully applied to amniotic fluid from suspected cocaine abusers.     

An improved method for complement subcomponent C1R typing

An improved method has been developed for the reliable classification of different C1R genetic variant forms from human serum or plasma. The method combines the use of neuraminidase-digested samples followed by monodimensional isoelectric focusing in the pH range 5 to 8 followed by immunoblotting. The method yields a simple pattern, with one major band in homozygote and two major bands in heterozygote cases.     

Civil society and democracy in the third world: Ambiguities and historical possibilities

The terminology of “civil society” has gained currency in recent discussions of democratic movements around the globe. Although less grandiose in its implications than claims about the “end of history,” this terminology does suggest a certain universality in human experience. We argue that this claim of universality is warranted, but also problematic. We establish the relevance of our argument in reference to the literatures in African and Indian studies. We note first that the common employments of the concept ignore the theoretical and historical specificity of civil society: civil society is used to label any group or movement opposed to the state, regardless of its intent or character, or used so generically that it is indistinguishable from the term “society.” Instead, we argue that civil society is a sphere of social life, involving a stabilization of a system of rights, constituting human beings as individuals, both as citizens in relation to the state and as legal persons in the economy and the sphere of private association. Thus, we link the wide resonance of the concept to its embeddedness in the logic of liberal capitalist society and the capitalist global division of labor. This conception allows us to see that, although the emergence of a sphere of civil society involves at least minimal democranization and is supportive of struggles for further democratization, the status of democracy is also made quite problematic by the tensions endemic to liberal capitalism and the processes of uneven development within international capitalism. Our usage also allows us to distinguish more clearly movements dedicated to the construction of civil society from those that may count actually as counter-civil society movements. David L. Blaney received his M.A. and Ph.D. at the Graduate School of International Studies, University of Denver. He is on leave from Hanover College, Hanover, Indiana as a visiting scholar for the 1993–94 academic year at The Elliott School of International Affairs, George Washington University, Washington, D.C. 20052. His main research interests include international political economy, culture and international relations theory, and democratic theory. Mustapha Kamal Pasha received his M.A. and Ph.D. at the Graduate School of International Studies, University of Denver. Currently, he is an assistant professor in the School of International Service, American University, Washington, D.C. 20016. His main research interests include international political economy, with particular regard to the Third World, and South Asian politics.     

G3M T typing by dot immunobinding with monoclonal anti-G3M T antibody

Dilutions of 100 serum samples of various GM phenotypes were dotted onto a nitrocellulose membrane. The serum dot-blots were detected with peroxidase-labeled anti-G3M T monoclonal antibody (anti-G3M T MAb). Up to a 1 : 256 dilution could be G3M T-typed correctly. By use of anti-G3M T MAb and peroxidase-labeled anti-mouse IgG or the biotin-avidin system instead of use of labeled anti-G3M T MAb, up to a 1 : 512 or 1 : 1024, respectively, dilution was typable. As with previous work with G3M G MAb, the dot immunobinding (DIB) method for G3M T typing was found very simple and practical.     

Children of Alcoholism: Implications For Juvenile Justice

Alcoholism and drug addiction affects the entire family. At least 22 million Americans have been raised in an alcoholic environment. Parental alcoholism and/or addiction can have profound emotional and psychological scars on the children. These homes are often characterized by emotional and or physical insecurity, loneliness, terror and inconsistency. The children from these homes often are extremely insecure have low self-esteem, and have a variety of emotional problems. Adolescents from addictive homes are over-represented in juvenile court as status offenders, delinquents and abuse and neglect cases. This article will discuss this issue and implications for the juvenile justice system and personnel working with these youth     

Gm/Km typing of bloodstains in U-bottom microtiter plates

A technique was developed for Gm/Km typing of bloodstains and sera in U-bottom microtiter plates. Gm/Km typing of sweat and urine samples was also attempted with limited success.     

Chronic disease and disadvantage: the new politics of HIV infection

HIV infection is now perceived as the end stage of a chronic disease that is spreading most rapidly among blacks and Hispanics. The politics of the HIV epidemic in the 1980s were dominated by four interacting factors: fear and fascination; who had the disease and to whom it seemed to be spreading; the endemic problems of United States social policy; and the impact on policy of advances in scientific knowledge. This paper analyzes the political history of each of these factors and describes the dominant policies of the federal government and the states regarding HIV in the areas of surveillance, prevention, research, and financing. Four uncertainties will have a profound influence on the future politics of the HIV epidemic: how the states and the federal government will address the general problems of paying for the care of people with chronic diseases and providing access to care for the uninsured and the underinsured; the number and distribution of the sexual behaviors that transmit infection with HIV and the effectiveness of policies to persuade people to modify these behaviors; precisely who uses addictive drugs and the effectiveness of measures to change their behavior; and the natural history of the virus.