Fingerprint enhancement revisited and the effects of blood enhancement chemicals on subsequent profiler Plus fluorescent short tandem repeat DNA analysis of fresh and aged bloody fingerprints

This study was aimed at determining the effect of seven blood enhancement reagents on the subsequent Profiler Plus fluorescent STR DNA analysis of fresh or aged bloody fingerprints deposited on various porous and nonporous surfaces. Amido Black, Crowle's Double Stain. 1,8-diazafluoren-9-one (DFO), Hungarian Red, leucomalachite green, luminol and ninhydrin were tested on linoleum, glass, metal, wood (pine, painted white), clothing (85% polyester/15% cotton, 65% polyester/35% cotton, and blue denim) and paper (Scott 2-ply and Xerox-grade). Preliminary experiments were designed to determine the optimal blood dilutions to use to ensure a DNA typing result following chemical enhancement. A 1:200 blood dilution deposited on linoleum and enhanced with Crowle's Double Stain generated enough DNA for one to two rounds of Profiler Plus PCR amplification. A comparative study of the DNA yields before and after treatment indicated that the quantity of DNA recovered from bloody fingerprints following enhancement was reduced by a factor of 2 to 12. Such a reduction in the DNA yields could potentially compromise DNA typing analysis in the case of small stains. The blood enhancement chemicals selected were also evaluated for their capability to reveal bloodmarks on the various porous and nonporous surfaces chosen in this study. Luminol. Amido Black and Crowle's Double Stain showed the highest sensitivity of all seven chemicals tested and revealed highly diluted (1:200) bloody fingerprints. Both luminol and Amido Black produced excellent results on both porous and nonporous surfaces, but Crowle's Double Stain failed to produce any results on porous substrates. Hungarian Red, DFO, leucomalachite green and ninhydrin showed lower sensitivities. Enhancement of bloodmarks using any of the chemicals selected, and short-term exposure to these same chemicals (i.e., less than 54 days), had no adverse effects on the PCR amplification of the nine STR systems surveyed (D3S 1358, HumvWA, HumFGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820) or of the gender determination marker Amelogenin. The intensity of the fluorescent signals was very similar and the allele size measurements remained constant and identical to those of untreated bloody fingerprints. No additional background fluorescence was noted. Continuous exposure (for 54 days) to two of the seven enhancement chemicals selected (i.e., Crowle's Double Stain and Hungarian Red) slightly reduced the amplification efficiency of the longer STR loci in profiles of fresh and 7 to 14-day-old bloodprints. This suggests that long-term exposure to these chemicals possibly affects the integrity of the DNA molecules. This study indicates that significant evidence can be obtained from fresh or aged bloody fingerprints applied to a variety of absorbent and nonabsorbent surfaces which are exposed to different enhancement chemicals for short or long periods of time. It also reaffirms that PCR STR DNA typing procedures are robust and provide excellent results when used in concert with fluorescence-based detection assays after fingerprint identification has taken place.     

Ketamine in non-hospital and hospital deaths in New York City

We reviewed all ketamine-positive deaths (87) examined at the New York City Office of Chief Medical Examiner over a two-year period (1997 to 1999). There were 15 non-hospital deaths with 12 due to acute multidrug intoxications, one due to sarcoidosis, and two due to physical injury (blunt and thermal). In no instance was a fatal intoxication caused exclusively by ketamine. Opiates (10/15), followed by amphetamines (7/15) and cocaine (6/15), were the most frequent co-intoxicants. Ethanol was found in only one death. The race of all decedents was white and the majority were men (11/15) between the ages of 18 and 30 years. The remaining 72 instances of positive ketamine findings were hospital deaths following surgical procedures or burns.     

B. Reviews: general

EUROPE'S FUTURE IN THE ARAB VIEW. Edited by Dieter Beilenstein. (Paper of the Institute for International Relations, No.7), Saarbrücken, Breitenbach, 1981. pp.163.

A CHANGING IMAGE: AMERICAN PERCEPTIONS OF THE ARAB‐ISRAELI DISPUTE. By Richard H. Curtiss. Washing D.C., American Educational Trust, 1982.

THE BATTLE OF BEIRUT: WHY ISRAEL INVADED LEBANON. By Michael Jensen. London, Zed Press, 1982.

FIRE OF ISLAM. By Desmond Meiring. London, Wildwood House, 1982.

PUBLIC DUTIES IN ISLAM: THE INSTITUTION OF THE HISBA. By Ibn Taymiya, Al‐Shaykh Al‐Imam Taqi Al‐Din Ahmad (trans. Mukhtar Holland). (Islamic Economic Series 3), Leicester, The Islamic Foundation, 1982.

ISSUES IN ISLAMIC BANKING: SELECTED PAPERS. By M.N.Siddiqui. (Islamic Economic Series, 4), Leicester, The Islamic Foundation, 1983.

REGESTEN PUBLIZIERTER SAFAWIDISCHER HERRSCHERURKUNDEN: ERLASSE UND STAATSSCHREIBEN DER FRUHEN NEUZEIT IRANS. By Renate. Schimkoreit. (Islamkuhdliche Untersuchungen, Bd 68), Berlin, Klaus Shwarz Verlag, 1982. pp. 552. DM.87.

THE HISTORY OF THE BRITISH PETROLEUM COMPANY.‐ VOLUME I: THE DEVELOPING YEARS 1901–1932.. By R.W.Ferrier. Cambridge University Press, 1982. pp.801. £37.50.

THE CHALLENGE OF ENERGY: POLICIES IN THE MAKING. Edited by Mohammad W. Khouja. (Energy Resources and Policies of the Middle East and North Africa.) London‐New York, Longman, 1981. pp.127. Paperback.

THE CAMBRIDGE HISTORY OF IRAN. Volume 3: THE SELEUCID, PARTHIAN AND SASANIAN PERIODS. Edited by Ehsan Yarshater. Vol. 3(1), pp. lxxv, 624, 48 pl., 4 line drawings, 13 maps; vol.3(2), pp.xix, thereafter pagination continuous with that of 3(1), ending at p.1488, 99 pl. (4 in colour), 25 line drawings, 3 maps. Cambridge, Cambridge University Press, 1983. £37.50.

TA ‘LIKI‐ZADE'S ?EHNAME‐YI HÜMAYUN: A HISTORY OF THE OTTOMAN CAMPAIGN INTO HUNGARY 1593–94. Edited with introduction and notes by Christine Woodhead. (Islamkundliche Untersuchungen, Band 82.) vii, pp.421, map. Berlin, Klaus Schwarz Verlag, 1983.

OTTOMAN YEAR BOOKS (SALNAME AND NEVSAL). Compiled by Hasan Duman. Istanbul, Research Centre for Islamic History, Art and Culture (IRCICA), 1402/1982. pp.144.

THE CRISIS OF TURKISH DEMOCRACY. By C.H.Dodd. Hull, Eothen Press, 1983. pp.136. £9.50 (cloth); £4.95 (paperback).

CULT, GHETTO AND STATE: THE PERSISTENCE OF THE JEWISH QUESTION. By Maxime Rodinson. London, Al Saqi Books, 1983. pp.239. £5.95.     

Embracing austerity? An ethnographic perspective on the Latvian public’s acceptance of austerity politics

ABSTRACT

Latvian austerity policy following the 2008 economic crisis has been touted as a success story by some and critiqued as a socially costly experiment by others. It has remained a puzzle, however, how such harsh socio-economy policies were possible without causing sustained popular protests. Drawing on ethnographic research at an unemployment office in Riga in the aftermath of the crisis, this article considers austerity as a political and moral phenomenon. I argue that welfare policies played an important role in disciplining the parts of the population most adversely affected by the crisis by framing post-crisis precarity as a matter of individual responsibility. Furthermore, this disciplining worked because it was underpinned by a particular moral discourse that I call ‘a discourse of freedom.’ Thus, this historically and culturally-shaped moral economy helped not only secure the implementation of post-crisis austerity in a way that yielded little sustained public resistance but also helped legitimate it.     

Child barbarity and torture: a case report

Penile strangulation with a strand of hair, acting as a tourniquet, and perforation of the eyes with a knife are uncommon accidental injuries in children. In such a situation, a mistreatment must be evoked. We present a case of barbarity and torture involving a young boy 3 years old. He was a victim of penis strangulation and perforation of the eyes, performed on previous surgical and infectious lesions. The mistreatment was performed by his mother's boyfriend. The perpetrator used the previous lesions to hide his misdeed. This amazing case of mistreatment points out the violence of the wounds and their being performed on previous lesions.     

The role of competition in health care: a Western European perspective

The Federal Trade Commission and Department of Justice 2004 report Improving Health Care: A Dose of Competition expresses a clear allegiance to competition as the organizing principle for health care. In Europe, by contrast, the key organizing principle of health care systems is solidarity. Solidarity means that all have access to health care based on medical needs, regardless of ability to pay. This is not to say that competition is not important in Europe, but competition must take place within the context of solidarity. This article critiques the report from a European perspective, describes the role of competition in Europe (focusing in particular on European Union law), and suggests that the United States could learn from the European perspective.     

Validation of Large White Pig as an animal model for the study of cannabinoids metabolism: application to the study of THC distribution in tissues

This study presents a new animal model, the Large White Pig, which was tested for studying cannabinoids metabolism. The first step has focused on determination of plasma kinetics after injection of Delta(9)-tetrahydrocannabinol (THC) at different dosages. Seven pigs received THC by intravenous injections (50, 100 or 200 microg/kg). Plasma samples were collected during 48 h. Determination of cannabinoids concentrations were performed by gas chromatography/mass spectrometry. Results showed that plasma kinetics were comparable to those reported in humans. Terminal half-life of elimination was 10.6 h and a volume of distribution of 32 l/kg was calculated. In a second step, this model was used to determine the kinetic profile of cannabinoids distribution in tissues. Eight Large White male pigs received an injection of THC (200 microg/kg). Two pigs were sacrificed 30 min after injection, two others after 2, 6 and 24 h. Different tissues were sampled: liver, kidney, heart, lung, spleen, muscle, fat, bile, blood, vitreous humor and several brain areas. The fastest THC elimination was noted in liver tissue, where it was completely eliminated in 6 h. THC concentrations decreased in brain tissue slower than in blood. The slowest THC elimination was observed for fat tissue, where the molecule was still present at significant concentrations 24 h later. After 30 min, THC concentration in different brain areas was highest in the cerebellum and lowest in the medulla oblongata. THC elimination kinetics noted in kidney, heart, spleen, muscle and lung were comparable with those observed in blood. 11-Hydroxy-THC was only found at high levels in liver. THC-COOH was less than 5 ng/g in most tissues, except in bile, where it increased for 24 h following THC injection. This study confirms, even after a unique administration, the prolonged retention of THC in brain and particularly in fat, which could be at the origin of different phenomena observed for heavy users such as prolonged detection of THC-COOH in urine or cannabis-related flashbacks. Moreover, these results support the interest for this animal model, which could be used in further studies of distribution of cannabinoids in tissues.     

Fatal accidental intrathecal injection of vindesine

ABSTRACT: A 25-year-old woman being treated for non-Hodgkin's lymphoma was accidentally given vindesine intrathecally. The error was recognized immediately and a spinal cord washing was performed through syringing with isotonic saline. However, the patient died 6 weeks later with increasing paralysis, which was followed by neurologic failure. The deceased was autopsied and the central nervous system was removed for a microscopic examination. The results showed microscopic lesions extending from the lumbar to the thoracic portion of the spinal cord, which included pseudocystic transformation of the cells, degeneration of myelin, and microhemorrhages. The brain was edematous and, in the cerebellum, the vermis showed a loss of granule and Purkinje cells. The authors compare this report on vindesine toxicity with cases in the literature involving vincristine. The treating physician admitted responsibility and was sentenced to both a fine and imprisonment.