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H C Lee E M Pagliaro K M Berka N L Folk D T Anderson G Ruano T P Keith P Phipps G L Herrin D D Garner 《Journal of forensic sciences》1991,36(2):320-330
Deoxyribonucleic acid (DNA) was isolated from a number of spongy and compact human bone tissue specimens, and the yield was estimated on a "per milligram of starting tissue" basis. DNA was, in addition, isolated from a number of corresponding blood and bone tissue specimens. Spectrophotofluorometry and ethidium bromide visualization on minigels were used to estimate the quantity and degree of degradation of DNA. The DNA from several blood-bone pairs is shown to give concordant restriction fragment length polymorphism (RFLP) typing results by two different typing protocols with five different single-locus probes. DNA from several additional blood-bone pairs is shown to give concordant results for human leucocyte antigen (HLA)-DQ alpha phenotypes following polymerase chain reaction (PCR) amplification and hybridization to specific allele-specific oligonucleotide (ASO) probes, and for the variable numbers of tandem repeats (VNTR) length polymorphisms 3' to the human apolipoprotein B (APOB) gene following PCR amplification with specific primers and analysis of the products by electrophoresis and ethidium bromide visualization. 相似文献
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The Institutional Basis of Secessionist Politics: Federalism and Secession in the United States 总被引:1,自引:0,他引:1
According to the literature on secession, the most importantdeterminant of secessionist sentiment is a high level of grievanceexperienced by the would-be secessionist group. However, therationale behind using secession (as opposed to another strategy)as a grievance-amelioration strategy remains almost completelyunexplained. This article contends that the institutional settingin which political conflict and grievance are experienced playsa crucial role in whether secession is conceived of as a viableoption. This contention is tested by examining the institutionalenvironment of federalism and the role it played in secessionin the United States in 1860 and 1861. The United States wasuniquely vulnerable to secessionist pressuresas are allfederal states, potentiallybecause of institutional structuresthat gave constituent states a high level of institutional authorityand capacity; this, in turn, contributed to a discourse of states'rights that included the contested right to secede. 相似文献
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From hegemonic to inclusive masculinities in English professional football: marking a cultural shift
AbstractIn this article, we conduct a generational study of measuring progress towards the inclusion of homosexuality in professional sport. We accomplish this by interviewing an openly gay football player, Anton Hysén, in the year 2017, using the precise same questions about the relationship between his homosexuality and sport that were asked of the first openly gay professional footballer in 1992, Justin Fashanu. We thus measure difference in attitude according to the 25 years that have elapsed between the interviews. Our results emphasize two themes: First, the near-total acceptance of being gay within professional football today, compared to 25 years ago; and second, the social praise that is levied upon today’s openly gay sportsmen, compared to the social stigma placed upon Fashanu. 相似文献
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Margaret Poulos 《澳大利亚政治与历史杂志》2000,46(3):418-427
This paper focuses on the recovery of the experience of partisan women who fought in the lines of the communist-led Greek Democratic Army (GDA) during the Greek Civil War (1946–1949). It is an extract from a research project which examines the extent to which the symbolism of Greek women in arms has informed the various waves of feminist discourse which have emerged since the founding of the modern Greek nation state. More specifically this work employs the image of the Greek woman warrior as an analytical category to investigate the relationship between militarism, nationalism and Greek feminist politics in relation to key nation-building conflicts of the modern Greek period. As such it belongs to the realm of scholarship informed by an understanding of 'gender' and 'nation' as constructed and contested relational systems of cultural and social meanings. Together the two systems not only shape the political culture in historically specific ways but also legitimate and limit the access of (groups of) people — women as well as men — to national movements as well as to the resources of the nation-state. This paper concentrates specifically on the heavily mythologised women of the GDA. It pays special attention to the historical association of their rebellion with national citizenship rights in post-war Greece. 相似文献