Detection of a primer-binding site polymorphism for the STR locus D16S539 using the Powerplex 1.1 system and validation of a degenerate primer to correct for the polymorphism

Quality assurance samples submitted from the NCSBI as part of a contract with TBTG to outsource DNA Database samples showed unexpected discrepancies for the locus D16S539 when all other loci yielded identical results. Discrepancies observed included allele drop out and an imbalance in sister alleles with samples returned from TBTG. This led to a comprehensive review of the technical procedures used between the two laboratories to determine the cause of the discrepancies noted for the locus D16S539, since both laboratories were using the PowerPlex 1.1 typing kit from the Promega Corporation. The NCSBI and the TBTG utilize different extraction methods (organic extraction vs. FTA) and amplification conditions (AmpliTaq vs AmpliTaq Gold), respectively, so the exact cause of discrepancy observed was not immediately apparent. Experiments at the NCSBI associated the observed allele drop out and the imbalance of the sister alleles with the use of AmpliTaq Gold and a hot start procedure. Sequencing data revealed that a point mutation resides on the D16S539 primer-binding site that reaches polymorphic levels in African-American populations. This led to the development of a degenerate primer by the Promega Corporation to detect "missing" alleles when AmpliTaq Gold is used. The degenerate primer was then thoroughly tested to show its efficacy in detecting the "true" D16S539 profile when used.     

Forensic comparison of soils by bacterial community DNA profiling

This preliminary investigation has shown that a soil microbial community DNA profile can be obtained from the small sample of soil recovered from the sole of a shoe, and from soil stains on clothing. We have also shown that these profiles are representative of the site of collection and therefore could potentially be used as associative evidence to prove a link between suspects and crime scenes. Soil community profiles were obtained using the T-RFLP fingerprinting method that uses fluorescent primer technology and semi-automated analysis techniques similar to those used in human DNA profiling in forensic laboratories.     

Policy feedback and public opinion: the role of employer responsibility in social policy

This study extends the literature on policy feedback and explores the extent to which public attitudes reflect learning from past government initiatives. We analyze the ways in which feedback mechanisms affecting public attitudes may differ from those earlier identified in the literature. We apply this general analytic framework to help explain variation in public attitudes toward private employer involvement in health care, explore possible causal pathways, and offer some preliminary empirical tests of these hypotheses. There are different levels of public support for the notion of employer obligation involving medical care, long-term care, and the treatment of substance abuse. Our evidence suggests that lessons about the performance of institutions in each of these policy domains represent the most important effect of existing policy on public attitudes. Furthermore, these differences correspond to what one would expect based on our model of policy feedback and cannot be explained by other plausible sources of policy legitimacy.     

Autopsy confirmation of severe pulmonary interstitial fibrosis secondary to Munchausen syndrome presenting as cystic fibrosis

Chronic factitious disorder with physical symptoms, or Munchausen syndrome, is a well-recognized but uncommonly diagnosed psychiatric condition characterized by the deliberate production of signs and symptoms of disease in order to receive medical attention. Clinical suspicion of this disease is rarely confirmed by autopsy, as the patients usually do not die as a consequence of feigning illness. Here we report the autopsy confirmation of a case of a suspected Munchausen syndrome patient who presented with a history of cystic fibrosis. Examination of the lungs demonstrated extensive severe interstitial fibrosis, and polariscopic examination revealed a large quantity of crystalline material throughout the tissue; X-ray diffraction identified the material as talc. Synopses of published cases of Munchausen syndrome presenting as cystic fibrosis, and cases of Munchausen syndrome with pulmonary talcosis are presented as part of the discussion.     

Adolescent Impulsivity: Findings From a Community Sample

Impulsivity is central to several psychopathological states in adolescence. However, there is little consensus concerning the definition of impulsivity and its core dimensions. In response to this lack of consensus, Whiteside and Lynam (2001, Pers. Individ. Differ. 30, 669–689) have developed the UPPS Impulsive Behavior Scale, which is able to distinguish 4 dimensions of impulsivity in adults: Urgency, lack of Premeditation, lack of Perseverance, and Sensation seeking. The question arises of whether these 4 dimensions also exist in adolescents and also of whether gender differences can be observed. A sample of teenagers (314 girls and 314 boys) completed a French version of the scale (Van der Linden et al., Eur. J. Psychol. Assess., 2005). Based on exploratory and confirmatory analyses, the 4-factor model is replicated in girls, boys, and the whole sample. Concerning gender differences, girls have a higher score for Urgency and boys a higher score for Sensation seeking. Overall, this study suggests that the UPPS is a promising tool for studying impulsivity in adolescence. Assistant, Child and Adolescent Psychology Unit, University of Geneva, Switzerland, PhD in preparation: Impulsivity, cognitive biases, cognitive deficits, and antisocial behavior in adolescence. Received PhD in 1988 from the University of Liège, Belgium. Major research topics are cognitive biases and cognitive deficits in psychopathology, impulsivity and executive functions, memory deficits in amnesia and Alzheimer's disease, and cognitive rehabilitation in schizophrenia and Alzheimer's disease.     

The Impact of School Mental Health Services on Out-of-School Suspension Rates

The current study investigated whether the presence of school-based mental health clinicians in an urban school district was associated with reduced out-of-school suspension rates. Forty one elementary schools with expanded school mental health (ESMH) programs were compared to 41 schools without ESMH programs. Correlational analyses revealed associations between suspension outcomes and several school-level demographic variables including school attendance rate, percent of students in poverty, and percent of nonwhite students in the school. After controlling for these variables, however, results of stepwise linear regressions demonstrated no significant differences between ESMH and non-ESMH schools on suspension outcomes. Results of the current study suggest that the presence of ESMH clinicians will not necessarily impact suspension rates in an elementary school. Targeted and well-implemented approaches to address behaviors that lead to suspension, or school- and system-level policies to provide alternatives to suspensions, will likely be a better route to achieving this outcome.     

Informing Juvenile Justice Policy: Directions for Behavioral Science Research

Recent policy initiatives threaten to reduce the rehabilitative mission of the juvenile court or eliminate the court entirely. This article lays out a framework for an empirical assessment of these developments. It first evaluates the available and potential empirical support for three hypotheses about juveniles that might justify maintaining a separate, rehabilitation-oriented juvenile justice system: the hypotheses that, compared to adults, juveniles are more treatable, less culpable, and less deterrable. On the assumption that the continued existence of a rehabilitation-oriented juvenile court can be justified, it then provides suggestions as to how existing intervention strategies for juveniles could benefit from research attention to several substantive and methodological issues. These include refining outcome criteria and sampling strategies, matching offender and program characteristics, reexamining intervention efficacy, and focusing on decision makers and resource allocations.     

Validation of the Propensity for Abusiveness Scale in Diverse Male Populations

The Propensity for Abusiveness Scale (PAS; Dutton, 1995) was designed as a self-report perpetrator profile for intimate abusiveness. It was empirically validated through reports of abuse by intimate partners. The original PAS (Dutton, 1995) was given to 144 men in treatment for partner abuse and 44 demographically matched controls. It correlated significantly with partner reports of abusiveness and correctly classified men 82.2% of the time, as one standard deviation above or below the mean partners' report score for abusiveness. In the present study, the PAS was given to clinical outpatients, gay males, male college students, and a group of spousal assaulters. A criterion measure for abusiveness (the Psychological Maltreatment of Women Inventory; Tolman 1989, or the Psychological Maltreatment Inventory; Kasian & Painter, 1992) was collected from intimate partners. In all groups, the PAS correlated significantly with partners' reports of both physical and psychological abusiveness on subscales of the criterion measures: Dominance/Isolation and Emotional Abuse. For the college students and wife-assault groups, a new criterion measure was used: the Severity of Violence Against Women Scale. The PAS correlated significantly with partners' reports of threats and violence measured by this scale. The PAS appears to provide a nonreactive assessment instrument that is a strong predictor of intimate abusiveness across a variety of populations.