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Robert S. McLaren Martin G. Ensenberger Bruce Budowle Dawn Rabbach Patricia M. Fulmer Cindy J. Sprecher Joseph Bessetti Terri M. Sundquist Douglas R. Storts 《Forensic Science International: Genetics Supplement Series》2008,2(4):257-273
Several laboratories have reported the occurrence of a split or n − 1 peak at the vWA locus in PowerPlex® 16 and PowerPlex® ES amplification products separated on 4- and 16-capillary electrophoresis instruments. The root cause of this artifact is post-PCR reannealing of the unlabeled, unincorporated vWA primer to the 3′-end of the tetramethylrhodamine (TMR)-labeled strand of the vWA amplicon. This reannealing occurs in the capillary post-electrokinetic injection. The split peak is eliminated by incorporation into the loading cocktail of a sacrificial hybridization sequence (SHS) oligonucleotide that is complementary to the vWA primer. The SHS preferentially anneals to the primer instead of the TMR-labeled strand of the vWA amplicon. In addition, the n − 10/n − 18 artifact that may be seen at the vWA locus was determined to be due to double-stranded amplicon formed post-electrokinetic injection into the capillary. This was also eliminated by adding in two Complementary Oligo Targets (COT1 and COT2) in addition to the SHS oligonucleotide into the loading cocktail. These three oligonucleotides are complementary to the 33 bases at the 5′-end of the unlabeled vWA amplicon strand and the 60 bases at its 3′-end and therefore compete for hybridization to the TMR-labeled amplicon strand. Incorporation of these three oligonucleotides in the Internal Lane Standard 600 (ILS600) eliminate both the split peak and n − 10/n − 18 artifact in PowerPlex® 16 and PowerPlex® ES amplification products without affecting sizing of alleles at the vWA locus or any locus in the PowerPlex® 16, PowerPlex® Y, PowerPlex® ES, AmpFlSTR® Profiler Plus® ID, AmpFlSTR® Cofiler®, and AmpFlSTR® SGM Plus® kits. 相似文献
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Although there is an already large British literature both supporting and attacking left realism, and a growing North American interest on the subject among criminologists, there has been surprisingly little written which attempts to locate both the strengths and weaknesses of the left realist position on crime control. Perhaps the place where the left realists may be weakest is in response to a feminist critique. Actually, it is not only left realism but the socialist left in general which has been unsuccessful in providing adequate responses to the issues brought forth by feminists. This paper attempts to locate the position of left realism within the left criminology debate, and to find its strong and weak points. Further, it attempts to explicate the feminist critique, and to suggest responses critical criminologists might explore, such as those proposed by peacemaking criminology. *** DIRECT SUPPORT *** AW502012 00004 相似文献
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Netherlands International Law Review - The Russia–Ukraine conflict is also being fought in the maritime dimension. Regarding methods of warfare, of particular note has been the possible... 相似文献
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Martin Mathieu Nganmeni Zéphirin Piggins Ashley Tchouante Élise F. 《Public Choice》2022,190(3-4):301-316
Public Choice - Pure-strategy Nash equilibria almost never exist in spatial majority voting games when the number of positional dimensions is at least two, as the majority core is typically empty... 相似文献
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Karin D. Martin 《Law & policy》2023,45(4):459-481
Fiscal contrition refers to the phenomenon of policy-makers becoming aware of the social costs of fines and fees, recognizing a need to reduce those costs, and taking action to do so. In order to reveal the occurrence of fiscal contrition, this analysis examines detailed budget data from three U.S. counties. Findings indicate a dominance of predatory over punitive monetary sanctions in county budgets. That is, fines and fees that extract revenue from a justice-involved population are more common than those with social control objectives. The analysis also reveals patterns and nuances in fine and fee usage and the revenue they produce, which illuminates pathways for reducing reliance on fine and fee revenue. This approach provides useful context for the burgeoning scholarship focused on the role of monetary sanctions in fueling social inequities. 相似文献
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