Rapid direct PCR for ABO blood typing

Many different molecular typing methods have been reported to complement routine serological ABO blood typing in forensics. However, these ABO genotyping methods are often time-consuming and call for an initial DNA isolation step that requires the use of expensive kits or reagents. We report here a rapid direct ABO genotyping method that eliminates the need for DNA extraction from fresh blood, hair, and body fluid stains before PCR. Using a fast PCR instrument and an optimized polymerase, the genotyping method-which employs a multiplex allele-specific primer set for the simultaneous detection of three single-nucleotide polymorphism (SNP) sites (nucleotides 261, 526, and 803)-identifies A, B, O01/O02, O03, and cis-AB01 alleles in around 70 min from sample collection to electropherogram. Not only will this ABO genotyping method be efficiently used in forensic practice for rapid screening of samples before full-blown multilocus short tandem repeat profiling, but it will also demonstrate an example of rapid direct genotyping of SNPs that offers the advantages of time- and cost-efficiency, convenience, and reduced contamination during DNA analysis.     

Imperialism in China: An Exchange

Abstract

From the viewpoint of those in control of state power, few things can be more frustrating than the relative autonomy of the populace in whose name political power is exercised. However, when a large number, preferably the majority, of those who cherish this autonomy are in need, an unquestionable reason for intervention is provided. When this state of need can be linked, if only in formal terms, to the low productivity of labor, a specific field of intervention is clearly defined. Finally, when relative autonomy, low labor productivity, and need can be shown to coincide in specific groups or social classes, the State and its allies are provided with the social, economic, and political legitimacy to intervene. It is then that their solicitude knows no bounds. Getting people to work more efficiently, more productively, becomes the major goal of society. While this preoccupation has always been present whatever the form of State power, it stands as the raison d’être of all contemporary authoritarian regimes, whether of the left or the right.     

Literatur

Ohne Zusammenfassung     

The Politics of the Human Rights Act

The Human Rights Act is the result of post-war changes in Europe. It is the consequence less of the campaigning of dedicated individuals and more of the change in the position of the United Kingdom in Europe and the world. Objections to incorporation of the Convention have given way to the desire to be like the rest of Europe. Traditional views of the judicial role have given way to a perception of the judiciary as the last bastion against an over-powerful executive, unchecked by Parliament.     

Victimization and fear of crime in an entertainment district crime “hot spot:” A test of structural-choice theory

Theories of criminal opportunity and criminal victimization (lifestyles, routine activities, and structuralchoice) all stress the convergence of motivated offenders, suitable targets, and the absence of guardianship in time and space. They each assert the chances of victimization increase as proximity to motivated offenders, exposure to highrisk environments, target attractiveness, and ineffective guardianship increase. This study tests Miethe and Meier’s structuralchoice theory by examining domainspecific victimization and fear of crime among patrons of an entertainment district crime “hot spot. ”Regression results show both victimization experience and fear of crime are associated significantly with indicators of proximity and guardianship, but not with exposure or target attractiveness. White patrons of this area are more likely to be victimized, nonwhites report significantly higher levels of fear. The research for this paper was supported by Grant No. 97PRWX0298 from the Office of Community Oriented Policing Services. Findings and conclusions of the research reported here are those of the authors and do not necessarily reflect the official position or policies of the U.S. Department of Justice.