Cardiac glycosides and metabolites--problems of recovery in tissue extracts. Separation of visible substance spots in the nanogram range (author's transl)]

The recovery measurements in rat tissues performed via i.p. injected radioactive digoxin derivates (3H-digoxin, 125J-digoxin derivative) showed that approximately 50% of the total glycoside content will be extracted. Thus, an addition of digoxin standards to drug-free tissues may lead to false negative determinations. By comparison of the radioactivity before and after extraction the following results were obtained: Recovery from tissues 3H-digoxin 50% 125J-digoxin 40% from serum 3H-digoxin 60% added to drug free tissue homogenates 3H-digoxin 85% After i.p. application of 15 mg/kg of beta-methyldigoxin to BD9 (Berlin)-rats the resulting tissue concentrations were extracted by Amberlite XAD-2. beta-Methyldigoxin and its metabolites digoxin and digoxinbisdigitoxide could be separated and distinguished from artifacts by fluorescence detection on HPTLC-plates with a detection limit of 60 ng/spot. Concentration determined by radioimmunoassay are in satisfactory agreement with HPTLC results.     

Forensic aspects of digoxin-poisoning: toxicological and morphological findings (author's transl)]

Case report. A 82 year old woman died 80 min after accidental ingestion of 5 mg beta-methyl-digoxin. The autopsy and the histological examination revealed non-specific alterations due to shock and preexisting coronary heart disease. Digoxin levels in various fluids and tissues were estimated by radioimmunoassay: bloodplasma 20--25 ng/ml, liquor 10--13 ng/ml, liver 100--110 ng/g, kidney 130--145 ng/g; the gastric fluid contained 0,6 mg. Forensic aspects of glycosid-intoxication, especially of the varying concentrations in different tissues, are discussed.     

The effect of a Charter of Rights on the policy-making role of Canadian courts

Abstract This paper explores the thesis that the main impact of the new constitutional Charter of Rights on the Canadian system of government is to increase the role of the judiciary in deciding how fundamental social and political values should be affected by public policy. The first part of the paper. by reviewing the significant role which trial and appeal courts already play in shaping public policy, aims at overcoming a tendency to exaggerate the change in our system of government resulting from the Charter. The second part identifies the factors which will influence the judicial response to the Charter and the policy areas in relation to which judicial interpretation will be most significant. The analysis shows that the Charter's major impacts will likely include much greater judicial supervision of police activity and centralization of policy-making under the leadership of the Supreme Court on questions of social justice and minority-language rights. It concludes that, while the Charter will increase Canadians' understanding of judicial policy-making, it represents a retreat from reliance on the political process to resolve divisive social issues. Sommaire. Le présent article examine la thèse selon laquelle le principal effet de la nouvelle Charte des droits constitutionnels sur le système de gouvemement canadien sera d'accroître le rôle du pouvoir judiciaire dans la détermination de la façon dont les valeurs sociales et politiques fondamentales devraient être affectées par les politiques publiques. La première partie de l'article passe en revue Ie rôle important que jouent déjà les tribunaux et les cours d'appel, ainsi que l'influence que ces institutions exercent sur la politique publique, ceci afin de neutraliser une tendance à exagérer les changements qu'apporterait la charte à notre système de gouvernement. La seconde partie identifie les facteurs qui pourront influencer la réponse judiciairs à la charte ainsi que les domaines politiques où l'interprétation judiciaire de cette charte aura le plus d'importance. L'analyse démontre que l'adoption de la charte entraînera des conséquences importantes, qui comprendront probablement une surveillance judiciaire des activités de la police beaucoup plus développée et une centralisation, sous la direction de la Cour suprême, de l'élaboration de politiques en matière de justice sociale et de droits linguistiques des minorités. L'auteur conclut que bien que la charte permettra aux Canadiens de mieux saisir le rôle de la Cour dans l'élaboration des politiques, elle représente un recul du recours au processus politique en ce qui concerne la résolution des questions sociales contentieuses.