Identification of furosemide in hair in a post‐mortem case by UHPLC‐MS/MS with guidance on interpretation

Testing for drugs in hair raises several difficulties. Among them is the interpretation of the final concentration(s). In a post‐mortem case, analyses revealed the presence of furosemide (12 ng/mL) in femoral blood, although it was not part of the victim's treatment. The prosecutor requested our laboratory to undertake an additional analysis in hair to obtain information about the use of furosemide. A specific method was therefore developed and validated to identify and quantify furosemide in hair by UHPLC‐MS/MS. After decontamination of 30 mg of hair, incubation in acidic condition, extraction with ethyl acetate, the samples were analyzed by UHPLC‐MS/MS. Furosemide was found in the victim's hair at 225 pg/mg. However, it was not possible to interpret this concentration due to the absence of data in the literature. Therefore, the authors performed a controlled study in two parts. In order to establish the basis of interpretation, several volunteers were tested (four after a single 20 mg administration and twenty‐four under daily treatment). The first part indicated that a single dose is not detectable in hair using our method. The second part demonstrated concentrations ranging from 5 to 1110 pg/mg with no correlation between dosage and hair concentrations. The decedent's hair result was interpreted as repeated exposures. In the case of furosemide analysis, hair can provide information about its presence but cannot give information about dosage or frequency of use.     

How Does Race Affect Perceptions of Police Trustworthiness?

While race is often discussed in relation to police, there is still little empirical evidence on the role of race in shaping citizens’ evaluations of government performance. Using an experimental design, this study examines how different levels of police performance affect perceptions of police trustworthiness and to which degree observed effects vary by individual race and across communities with different racial makeups. Specifically, we study the effect of different levels of performance on perceived trustworthiness in two communities—one predominantly African American and one predominantly White—and replicate across two samples: a sample consisting of primarily White participants and another consisting of only African American participants. Results indicate that the interaction between the racial composition of a community and individual race plays a critical role in shaping the effects of performance among White participants, who appeared more sensitive to community context than African American participants. Implications for research and practice are discussed.     

Social Anxiety and Aggression in Early Adolescents: Examining the Moderating Roles of Empathic Concern and Perspective Taking

Guided by a social information processing perspective, this study examined the unique and interactive contributions of social anxiety and two distinct components of empathy, empathic concern and perspective taking, to subsequent relational and overt aggression in early adolescents. Participants were 485 10- to 14-year old middle school students (54% female; 78% European-American) involved in two waves of a study with one year between each wave. Hierarchical regression analyses showed that higher levels of empathic concern were directly associated with decreases in subsequent relational and overt aggression one year later and buffered the impact of social anxiety on subsequent relational aggression. Although perspective taking did not moderate the impact of social anxiety on either form of aggression, it was a unique predictor of increased relational aggression one year later. Findings call for future research to assess both components of empathy separately as they relate to relational and overt aggression.     

The Effectiveness of Administrative Reform in New Democracies

Practitioners and scholars of postcommunist politics disagree on the accomplishments of administrative reforms in new Eastern European democracies. The transformation of the public sector after 1989 has aimed to consolidate the democratic process and enhance economic development. Skeptics, however, argue that administrative reforms face serious challenges in the context of economic liberalization, insufficient capacity for modernization, and cultural legacies of the past. The authors judge reform effectiveness by testing the impact of civil service reform on government transparency and foreign direct investment. The results of the empirical analysis confirm that once reform is adopted, administrations become more effective at reducing corruption and attracting investment. Despite the delays and difficulties of implementation, the adoption of reform is important in and of itself, and countries can expect positive results sooner than skeptics predict.     

Allele sharing in first-degree and unrelated pairs of individuals in the Ge F I AmpFlSTR Profiler Plus database

Eleven Italian forensic laboratories participated in a population study based on the AB Profiler Plus loci with proficiency testing. The validated database, including 1340 individuals, is available on-line. Tests for Hardy-Weinberg equilibrium, gametic unbalance, and heterogeneity of gene frequency were generally not significant. Gene frequencies at each locus were consistent with those of two previously published Italian studies, but different from a third. Individuals of each subsample were paired, and the total number of alleles shared across the nine loci was determined in each pair. The analysis was replicated over the total sample. In addition, two samples of mother-child pairs (N=315) and full-sib pairs (N=91) were subjected to allele sharing analysis. The resulting distributions were sufficiently distinct from the sample of unrelated pairs as to be of practical usefulness.     

Policy Target Populations and Public Participation in Agency Decision Making

Why are some government agencies more open to public input than others? Although many agree about the normative desirability of involving citizens in administrative decision making, there is significant variation across agencies in the extent of public participation. This article investigates the conditions under which public managers solicit greater public participation. We argue that, in addition to normative rationales, participation also serves instrumental considerations related to agency constituency. We draw on a rich body of literature examining participation in the policy process to develop empirically testable hypotheses about the patterns of participation in the administrative decision making of public agencies. Using data on the approaches to gathering citizen input in the budget process at four state departments—environmental protection, transportation, child protective services, and corrections—we find that the characteristics of target populations (namely, their political power and social construction) are strong predictors of an agency's openness to the public.     

How do international human rights influence national healthcare provisions for irregular migrants?: A case study in France and the United Kingdom

Debates about human rights have often questioned their potential for generating rights at national levels. In this article, we use the case of irregular migrants' access to health care in the United Kingdom and France to explore the extent to which international human rights influence national health care provisions for irregular migrants. We explore the extent to which health care access and provision for irregular migrants in these two countries is in agreement with international human rights. In so doing, we examine what constitutes an infringement of the international human right to health care. Finally, we sketch out some hypotheses about the role played by different state structures in the implementation of human rights norms, comparing the United Kingdom with France. We argue that, although international human rights often have a largely symbolic role in nation-state jurisdiction, they may sometimes represent a force for change.     

Fatal intoxication with 1,4-butanediol: Case report and comprehensive review of the literature

A fatal case of 1,4-butanediol (1,4-BD) oral ingestion is reported here, in which a 51-year-old man was found dead in his bed. According to the police report, the deceased was a known drug user. A glass bottle labeled (and later confirmed to be) “Butandiol 1,4” (1,4-BD) was found in the kitchen. Furthermore, the deceased's friend stated that he consumed 1,4-BD on a regular basis. The autopsy and histological examination of postmortem parenchymatous organ specimens did not revealed a clear cause of death. Chemical-toxicological investigations revealed gammahydroxybutyrat (GHB) in body fluids and tissues in the following quantities: femoral blood 390 mg/L, heart blood 420 mg/L, cerebrospinal fluid 420 mg/L, vitreous humor 640 mg/L, urine 1600 mg/L, and head hair 26.7 ng/mg. In addition, 1,4-BD was qualitatively detected in the head hair, urine, stomach contents, and the bottle. No other substances, including alcohol, were detected at pharmacologically relevant concentrations. 1,4-BD is known as precursor substance that is converted in vivo into GHB. In the synoptic assessment of toxicological findings, the police investigations and having excluded other causes of death, a lethal GHB-intoxication following ingestion of 1,4-BD, can be assumed in this case. Fatal intoxications with 1,4-BD have seldom been reported due to a very rapid conversion to GHB and, among other things, non-specific symptoms after ingestion. This case report aims to give an overview to the published of fatal 1,4-BD-intoxications and to discuss the problems associated with detection of 1,4-BD in (postmortem) specimens.