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101.
Fluorescence Imaging of Posterior Spiracles from Second and Third Instars of Forensically Important Chrysomya rufifacies (Diptera: Calliphoridae), 下载免费PDF全文
Danielle Flores M.S. Amy L. Miller M.S. Angelique Showman M.S. Caitlyn Tobita Lori M. N. Shimoda B.S. Carl Sung B.S. Alexander J. Stokes Ph.D. Jeffrey K. Tomberlin Ph.D. David O. Carter Ph.D. Helen Turner Ph.D. 《Journal of forensic sciences》2016,61(6):1578-1587
Entomological protocols for aging blowfly (Diptera: Calliphoridae) larvae to estimate the time of colonization (TOC) are commonly used to assist in death investigations. While the methodologies for analyzing fly larvae differ, most rely on light microscopy, genetic analysis, or, more rarely, electron microscopy. This pilot study sought to improve resolution of larval stage in the forensically important blowfly Chrysomya rufifacies using high‐content fluorescence microscopy and biochemical measures of developmental marker proteins. We established fixation and mounting protocols, defined a set of measurable morphometric criteria and captured developmental transitions of 2nd instar to 3rd instar using both fluorescence microscopy and anti‐ecdysone receptor Western blot analysis. The data show that these instars can be distinguished on the basis of robust, nonbleaching, autofluorescence of larval posterior spiracles. High‐content imaging techniques using confocal microscopy, combined with morphometric and biochemical techniques, may therefore aid forensic entomologists in estimating TOC. 相似文献
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There is widespread criticism of the behaviour of junior officials in post-communist Europe where they are often accused of treating citizens unfairly and soliciting presents or bribes to solve citizens' problems. To get the perspective of officials themselves we interviewed 1307 junior officials and state employees in Ukraine, Bulgaria, Slovakia and the Czech Republic. This paper focuses on their willingness to accept gifts from clients, their confessions to having done so, and the factors that lead some officials to accept gifts while others do not – factors such as occupational bargaining power and the (related) frequency of offers from clients, economic pressures, national and institutional cultures, fear of punishment, and moral perspectives on charging clients for favours. Economic pressures make officials more inclined to feel that they 'could not afford to refuse' gifts, but this motivation is only weakly related to actual behaviour. Occupational bargaining power and the frequency of offers from clients make them more likely to 'welcome' gifts or accept them 'out of politeness'– and these less excusable motivations are more strongly related to actual gift-taking. 相似文献
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James C. Miller III 《Public Choice》2010,142(3-4):407-408
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Norman H. Nie Darwin W. Miller III Saar Golde Daniel M. Butler Kenneth Winneg 《American journal of political science》2010,54(2):428-439
We propose a framework for understanding how the Internet has affected the U.S. political news market. The framework is driven by the lower cost of production for online news and consumers' tendency to seek out media that conform to their own beliefs. The framework predicts that consumers of Internet news sources should hold more extreme political views and be interested in more diverse political issues than those who solely consume mainstream television news. We test these predictions using two large datasets with questions about news exposure and political views. Generally speaking, we find that consumers of generally left‐of‐center (right‐of‐center) cable news sources who combine their cable news viewing with online sources are more liberal (conservative) than those who do not. We also find that those who use online news content are more likely than those who consume only television news content to be interested in niche political issues. 相似文献
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Chris Miller 《Orbis》2019,63(4):598-601
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This article analyzes the dynamics of contemporary cooperation and conflict in Japanese financial regulation through the prism of the jusen problem, the collapse of Japan's home mortgage lending industry in the 1990s. The jusen problem is one of the most striking examples of regulatory failure, strategic interest group bargaining, and large-scale dispute resolution in Japanese history. 相似文献