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11.
During a forensic toxicological GC/MS screening, changed compounds of hydroxyzine were found in a man's urine taken about 20 h after ingesting sake (Japanese liquor) and tranquilizers. The structures of those compounds were assigned to 4-chlorodiphenylmethane(I), p-chlorbenzophenone(III), norchlorcyclizine(IV), 7-(p-chloro-alpha-phenylbenzyl)-2-hydroxy-2H,3H-oxazolo-[3,2 -alpha] piperazine(V), 1-(p-chloro-alpha-phenylbenzyl)-4-methylpiperazine-3-one(VI), N-(p-chloro-alpha-phenylbenzyl)-N'-formylmethyl-N'- hydroxyethyl-1,2-ethenediamine(VII) and 1-(p-chloro-alpha-phenylbenzyl)-4-[2-ethoxy(2-ethoxy)-ethyl] piperazine(VIII) on the basis of the fragmentation patterns and relative retention times except VIII which was identified using the synthesized compound. I, V, VI, VII and VIII have not been reported previously, but the possibility of metabolites of hydroxyzine is still uncertain. 相似文献
12.
Nakanishi H Ohmori T Hara M Takada A Shojo H Adachi N Saito K 《Journal of forensic sciences》2011,56(Z1):S158-S161
We previously reported that detection of Streptococcus salivarius is feasible for proving the presence of saliva in a forensic sample. Here, a simple and rapid method for the detection of S. salivarius in forensic samples was developed that uses loop-mediated isothermal amplification (LAMP). The LAMP primer set was designed using S. salivarius-specific sequences of glucosyltransferase K. To simplify the procedure, the sample was prepared by boiling and mutanolysin treatment only, and the entire analytical process was completed within 2.5 h. The cut-off value was set at 0.1 absorbance units, measured at 660 nm, upon termination of the reaction. S. salivarius was identified in all saliva samples, but was not detected in other body fluids or on the skin surface. Using this method, S. salivarius was successfully detected in various mock forensic samples. We therefore suggest that this approach is useful for the identification of saliva in forensic practice. 相似文献
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14.
Y Hieda M Kageura K Hara M Takamoto Y Fukuma S Kashimura 《Forensic science international》1990,45(1-2):159-169
The present study was made on an experimental animal model of a death from anaphylaxis, in which postmortem changes in levels of histamine and 1-methylhistamine, in whole blood were measured. Instead of the usual immunological method administering compound 48/80, a degranulating agent of mast cell and the effect closely resembling the immuno-reaction, resulted in reliable death in a short time. The animals that died rapidly after the injection of compound 48/80, were found to have large increases in levels of histamine and 1-methylhistamine soon after the administration. These results were similar to the results of injecting histamine exogenously. On the other hand, the animals that died after a longer time showed no increases in levels of those amines within about 24 h, but 24 hours after death histamine levels were only increased tremendously without rise in 1-MHA levels. These phenomena closely resembled those in the control animals that were treated with overdoses of Nembutal. 相似文献
15.
Yuasa I Umetsu K Tsuchida S Nakamura H Endoh M Nakagawa M Irizawa Y 《Forensic science international》2002,126(3):248-251
In a Japanese paternity test, an alleged father was excluded only by reverse homozygosity of esterase D (ESD) phenotypes (mother, ESD 1; child, ESD 1; alleged father, ESD 2) out of 43 classical and DNA markers investigated. To solve the aberrant inheritance of the ESD phenotypes observed between them, fragments for all eight coding exons amplified by polymerase chain reaction (PCR) were subjected to DNA analysis. The child and alleged father shared a null allele, originating from ESD(*)1. It was characterized by having TGA for the stop codon instead of TCA for serine at codon 63. Thus, the sharing of a rare null gene, ESD(*)Q0(yonago), increased the probability of paternity. 相似文献
16.
Screening Test for Shed Skin Cells by Measuring the Ratio of Human DNA to Staphylococcus epidermidis DNA
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Hiroaki Nakanishi Ph.D. Takeshi Ohmori Ph.D. Masaaki Hara Ph.D. Shirushi Takahashi M.D. Ph.D. Akira Kurosu M.D. Ph.D. Aya Takada M.D. Ph.D. Kazuyuki Saito M.D. Ph.D. 《Journal of forensic sciences》2016,61(3):618-622
A novel screening method for shed skin cells by detecting Staphylococcus epidermidis (S. epidermidis), which is a resident bacterium on skin, was developed. Staphylococcus epidermidis was detected using real‐time PCR. Staphylococcus epidermidis was detected in all 20 human skin surface samples. Although not present in blood and urine samples, S. epidermidis was detected in 6 of 20 saliva samples, and 5 of 18 semen samples. The ratio of human DNA to S. epidermidisDNA was significantly smaller in human skin surface samples than in saliva and semen samples in which S. epidermidis was detected. Therefore, although skin cells could not be identified by detecting only S. epidermidis, they could be distinguished by measuring the S. epidermidis to human DNA ratio. This method could be applied to casework touch samples, which suggests that it is useful for screening whether skin cells and human DNA are present on potential evidentiary touch samples. 相似文献
17.
Application of mRNA Expression Analysis to Human Blood Identification in Degenerated Samples that were False‐negative by Immunochromatography,,
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Shusaku Matsumura M.S. Aya Matsusue Ph.D. Brian Waters M.S. Masayuki Kashiwagi M.D. Ph.D. Kenji Hara Ph.D. Shin‐ichi Kubo M.D. Ph.D. 《Journal of forensic sciences》2016,61(4):903-912
Forensic laboratories are often faced with cases in which methamphetamine hydrochloride‐mixed blood is unable to be identified as human blood by immunochromatography against human hemoglobin A0. The application of mRNA expression analysis to samples that showed a false‐negative with immunochromatography was investigated as an alternative approach that did not depend on the antigen–antibody reaction. Real‐time PCR was used to examine the expression levels of blood markers such as glycophorin A, spectrin beta, and hemoglobin beta. Hemoglobin beta was the only marker that was specifically detected in blood, while glycophorin A was useful for determining human specificity. Hemoglobin beta showed good detection sensitivity and was detectable in 37‐year‐old blood stains. Hemoglobin beta was exclusively detectable in methamphetamine hydrochloride‐mixed blood stains. Detergents and disinfectants did not significantly influence mRNA markers. The proposed mRNA expression analysis was suitable for human blood identification as an alternative method to immunochromatography. 相似文献
18.
Law and Critique - This paper provides a queer critique of the European Court of Human Rights’ use of ‘European consensus’ as a method of interpretation in cases concerning... 相似文献
19.
Kimie Hara 《The Pacific Review》2013,26(4):515-536
Abstract Whereas the Yalta System collapsed and the Cold War ended in the Euro‐Atlantic context, the basic structure of the Cold War remains in the Asia‐Pacific region, and still provides potential sources for future conflicts. This paper reconsiders the regional Cold War and its implications for current international politics in the Asia‐Pacific region. It argues that the relaxation of tensions seen since the late 1980s resembles the 1970s’ détente in the Euro‐Atlantic region, i.e. recognition of a Cold War political status quo, rather than the ‘end of the Cold War’. Along with political and ideological confrontations, significant elements within the Cold War structure in the Asia‐Pacific are regional conflicts among its major players. Their historical correlation within the San Francisco System has been neglected in past studies. The paper suggests that re‐examining the disputes within a multilateral framework may provide a key to a breakthrough in understanding the current status quo. 相似文献
20.
Hajime Mizukami M.D. Ph.D. Tomonori Nagai M.D. Ph.D. Shinjiro Mori M.D. Ph.D. Shuichi Hara Ph.D. Tatsushige Fukunaga M.D. Ph.D. Takahiko Endo M.D. Ph.D. 《Journal of forensic sciences》2010,55(6):1646-1648
Abstract: A 48‐year‐old woman was found dead on a chair in her living room. She had received dialysis every day because of chronic renal failure for the past 15 years. On a table beside her, there was a mirror and 10‐mL syringe on a napkin. A stopper was out of place in a portion of a three‐way blood access tube established in the right cervical region, and blood coagulation was noted in the lumen. There was a bloodstained measuring cup on the floor. Autopsy findings included a large number of shunt traces in the bilateral infraclavicular fossae and upper limbs, as well as the cervical blood access terminal reaching the right atrium via the internal jugular vein to superior vena cava. Various organs showed anemia. Neither a fatal lesion nor injury was noted in the main organs. Therefore, this patient may have committed suicide by self‐bloodletting via a cervical blood access. 相似文献