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111.
Evaluation of a Freezer Mill for Bone Pulverization prior to DNA Extraction: An Improved Workflow for STR Analysis
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Emely Morales Colón M.S. Mireya Hernández M.S. Mariel Candelario M.S. María Meléndez B.S. Tracey Dawson Cruz Ph.D. 《Journal of forensic sciences》2018,63(2):530-535
Traditional methods for bone pulverization typically generate heat, risking stability of DNA sample. SPEX? has developed cryogenic grinders which introduce liquid nitrogen to cool the sample and aid in the grinding process. In this study, the Freezer Mill 6970 EFM was used with two DNA extraction methods and routine downstream STR analysis procedures. DNA from as little as 0.1 g of bone powder was used to develop full STR profiles after freezer mill pulverization, and the method was reproducible. Further, no contamination was detected upon cleaning/reuse of the sample vials. There were no significant differences in DNA yield, STR alleles detected, or peak heights using the freezer mill as compared to traditional grinding, and successful DNA profiles were achieved from as low as 0.1 g of bone powder with this method. Overall, this work indicates that this cryogenic mill method may be used as a viable alternative to traditional tissue grinders. 相似文献
112.
Gisli H. Gudjonsson Jon Fridrik Sigurdsson Berglind Brynjólfsdóttir Hrafnhildur Hreinsdóttir 《心理学、犯罪与法律》2013,19(2):145-153
Abstract The aim of the study was to assess the relationship of compliance with anxiety, self-esteem, paranoid thinking, and anger. The Gudjonsson Compliance Scale (GCS), the State-Trait Anxiety Inventory (STAI), the Rosenberg Self-Esteem Scale, the Paranoia/Suspiciousness Questionnaire (PSQ), and the Novaco Anger Scale (NAS) were administered to 167 University students Compliance correlated positively with state and trait anxiety, low self-esteem, paranoia and suspiciousness, and negatively with acting out feelings of anger (the Behavioural Domain of the NAS). Multiple regression of the test scores showed that paranoid thinking was the single best predictor of compliance. The implications are discussed. It is important to replicate the study on special populations, such as offenders and psychiatric patients, who have problems with low self-esteem, paranoid thinking, and anger. 相似文献
113.
Francisco Bastida Bernardino Benito María-Dolores Guillamón 《International Public Management Journal》2013,16(4):484-499
ABSTRACT Public finance theories state that both political and socioeconomic factors must be considered in order to explain governments' finances. On the one hand, “partisan politics matters” thesis argues that progressive parties contribute to increase public deficit. On the other hand, Roubini and Sachs' weak government hypothesis (1989a, 1989b) states that the higher the government fragmentation, the higher spending, deficit and debt. Accordingly, our work evaluates whether municipal ideology and political strength have an impact on public expenditures and taxes. With this aim, we analyze a representative sample of Spanish municipalities (2,729) for the year 2005. We find an influence of the size of the political majority on the municipal financial situation. However, we do not find evidence of the impact of the government's political ideology. Economic and population variables are found highly significant. Our data also show a “flypaper” effect in the Spanish municipal sector. 相似文献
114.
David Ortiz Rodríguez Andrés Navarro Galera Antonio M. López Hernández 《International Public Management Journal》2013,16(4):371-398
ABSTRACT The usefulness of performance indicators increases when organizations compare their data. However, most discussions have failed to emphasize two lines of action that could improve the relevance of such comparisons: a) agreement among service managers on the indicators to be used in the evaluation; b) homogenization of the indicators to be used by organizations, and of the process used to calculate them. In this context, this paper proposes a methodology based on the participation of and consensus among managers, which helps to standardize the construction and presentation of performance indicators. The aim of this proposal is to improve the comparative evaluation of organizations. Finally, in order to test the proposal, a practical application is carried out on the local culture departments of nine large municipalities in southern Spain. 相似文献
115.
Manuel Pedro Rodríguez Bolívar Laura Alcaide Muñoz Antonio M. López Hernández 《International Public Management Journal》2013,16(4):557-602
ABSTRACT The search for enhanced transparency and accountability in government organizations has inspired studies to identify the key factors that facilitate greater disclosure of public financial information. With the advantages provided by the meta-analysis technique, applied to a sample of studies, we identify the most significant factors and incentives underlying the decisions adopted by public managers on policy strategies regarding information transparency and public responsibility. Our study shows the variables analyzed to be positively associated with the disclosure of public financial information, but also that this depends on the context in which the research is carried out. The most influential variables were the moderator variables of the administrative culture and of the measurement unit for the variables. 相似文献
116.
This contribution examines the impact of profound changes to agricultural policy implemented since 1988 on the livelihoods of Mexico's rural population. Detailed studies in four villages show that rural incomes are very unevenly distributed within communities leaving half of households in poverty. During the last decade key factors affecting village economies have been international and national, rather than specific changes to farm policy. Most changes have been to the detriment of the communities studied, but peasant households have adapted and survived, at a price. If the worst fears about the consequences of economic liberalisation have not been realised, neither have the hopes. Depressed markets for basic goods and services have limited the growth of the rural economy. Private investment and provision of services have not been stimulated. 相似文献
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Braulio Gómez Fortes Laura Cabeza Irene Palacios 《South European society & politics》2013,18(4):591-610
Confounding predictions, the 2012 Andalusian regional election resulted in a renewal of left-wing government as the conservative Popular Party failed to obtain an absolute majority of seats. The socialists, who had ruled the region for 30 years, came second but continued in power in coalition with the United Left, which increased its seat share. The article argues that the Andalusian election results should be understood in a multi-level governance perspective. For those who voted for the socialists, the hard austerity policies implemented by the Popular Party central government became more salient than the current performance of the regional government. The behaviour of unemployed voters is also key to understanding the election outcome. 相似文献