Elimination of 7-aminoflunitrazepam and flunitrazepam in urine after a single dose of Rohypnol

The hypnotic benzodiazepine flunitrazepam (Rohypnol) has been identified as the drug of choice for the purposes of "drugging" unsuspecting victims and raping them while they are under the influence of this substance. The objective of this paper was to study elimination of flunitrazepam and 7-aminoflunitrazepam in urine collected from ten healthy volunteers who received a single 2 mg oral dose of Rohypnol, to determine how long after drug administration 7-aminoflunitrazepam can be detected. A highly sensitive NCI-GC-MS method for the simultaneous quantitation of flunitrazepam (LOQ 100 pg/mL) and 7-aminoflunitrazepam (LOQ 10 pg/mL) in urine was developed. All samples were screened for benzodiazepines using optimized micro-plate enzyme immunoassay. The highest concentrations of 7-aminoflunitrazepam (70-518 ng/mL) and flunitrazepam (0.7-2.8 ng/mL) in urine were observed 6 h after drug administration in nine subjects and after 24 h in one subject. In six subjects 7-aminoflunitrazepam was detected up to 14 days after flunitrazepam administration, in one subject up to 21 days and in three subjects up to 28 days. In urine samples collected from six volunteers, flunitrazepam was detected three days after Rohypnol intake, in three subjects 24 h, and in one subject 5 days later. Benzodiazepine micro-plate enzyme immunoassay kit allowed the detection of flunitrazepam and metabolities 5 to 21 days after drug administration.     

Australia and the Emergence of the Modern Two-Party System

Although the Australian party system is accepted in some literature as a "classic" example of a two-party system, information about how this system came about does not feature prominently in international studies on political parties. It is here argued that Australia indeed blazed the trail for the two-party model by being the first to link parliamentary organisation directly to a mass electorate.     

House of Lords simplifies the law on patent entitlement

The House of Lords has held that, to claim entitlement to another'spatent or patent application, a person need only prove thathe was the inventor of the subject-matter of the patent, anddoes not also need to invoke ‘some other rule of law’as required previously by the Court of Appeal in Markem v Zipher[2005] RPC 31.     

Causal Attributions and Affective Responses to Provocative Female Partner Behavior by Abusive and Nonabusive Males

The present study examined the effects of the degree of female partner provocation on cognitive attributions and affective responses in verbally abusive and nonabusive college males. In Phase 1 (N = 116), subjects listened to audiotapes of hypothetical dating situations in which the female partner's behavior was nonprovocative or moderately provocative; in Phase 2 (N = 105), the female partner's behavior was nonprovocative or highly provocative. The major hypothesis was that abusive males would make greater negative intent and responsibility attributions and report more powerful feelings of jealousy, rejection, and abandonment in response to moderately and highly provocative partner behavior but not in response to nonprovocative partner behavior than would nonabusive males. Results from Phase 1 showed that abusive males reported reliably greater negative attributions and feelings of jealousy, rejection, and abandonment in response to moderately provocative partner behavior than did nonabusive males. No group differences were associated with nonprovocative partner behavior. Results from Phase 2 showed that abusive males attributed greater negative intent and feelings of rejection and abandonment to both highly provocative and nonprovocative partner behavior than did nonabusive males. Negative attributions and feelings of jealousy, rejection, and abandonment increased reliably from moderately provocative to highly provocative female behavior for abusive and nonabusive males, who differed reliably from each other. Implications for the assessment and treatment of abusive men were discussed.     

The recovery of latent fingermarks and DNA using a silicone-based casting material

There are many techniques available for the recovery of fingermarks at scenes of crime including the possibility of taking casts of the marks. Casts can be advantageous in cases where other destructive recovery techniques might not be suitable, such as when recovering finger marks deposited on valued or immobile items. In this research, Isomark (a silicone-based casting material) was used to recover casts of finger marks placed on a variety of substrates. Casts were enhanced using cyanoacrylate fuming. Good quality marks were successfully recovered from a range of smooth, non-porous surfaces. Recovery from semi-porous surfaces was shown to be inefficient. DNA was subsequently extracted from the casts using QIAamp Mini extraction kits, amplified and profiled. Full DNA profiles were obtained 34% of samples extracted.