Autopsy and genetic diagnosis of 21-hydroxylase deficiency with bilateral testicular tumors in a case under no medication for over one year

The autopsy findings of an adult patient with 21-hydroxylase deficiency are presented. Genetic analysis of the 21-hydroxylase gene (CYP21A2) was performed for accurate diagnosis of congenital adrenal hyperplasia (CAH), and bilateral testicular tumors were characterized. We report a 29-year-old Japanese man who was diagnosed with CAH (21-hydroxylase deficiency) in infancy and had continued steroid therapy until the age of 28. However, for more than one year, he had not been treated for CAH and was found dead. In the medico-legal autopsy findings, both adrenal glands were enlarged, and hypertrophy of adrenal cortices and bilateral testicular tumors positive for melan-A were observed. Genomic DNA was prepared from cervical lymph nodes collected during autopsy, and CYP21A2 was PCR amplified and sequenced directly using newly designed primers. From the morphological findings, the bilateral testicular tumors were considered to be adrenogenital syndrome (TTAGS). Through the whole sequence of CYP21A2, the intron 2 splice mutation (656)A to (656)G was found. TTAGS were thought to be adrenal rests enlarged by ACTH stimulus. From the autopsy findings and the result of genetic analysis, he was diagnosed with the salt-wasting form of 21-hydroxylase deficiency and his cause of death was presumed to be heart failure based on abnormal electrolytes.     

Simultaneous observation of catecholamine, serotonin and their metabolites in incised skin wounds of guinea pig.

In order to examine the vital reaction in wounds, catecholamines, serotonin (5-HT) and their metabolites in the incised skin wounds of guinea pigs were analyzed simultaneously by high-performance liquid chromatography (HPLC) using electrochemical detection (ECD). The principal changes in the levels of these compounds in vital wounds were as follows: a considerable decrease in norepinephrine (NE) content was observed 12-24 h after injury which persisted to at least 7 days. 3,4-Dihydroxyphenylacetic acid (DOPAC) decreased slightly for up to 30 min and then showed no significant difference compared with postmortem levels. Epinephrine and dopamine were barely detected by the HPLC-ECD method employed. 5-HT concentrations which showed an increase up to 24 h showed maximum levels 800 microns from the wound edge at 10 and 30 min after injury. 5-Hydroxyindoleacetic acid (5-HIAA) was significantly higher than the postmortem level over almost the entire period of these experiments. A 5-HIAA content of at least twice the postmortem level was observed 800 microns from the wound edge of a 10-min-old vital wound. Therefore, 5-HIAA is a likely candidate as a new marker for evaluating the vital reaction in wounds. The vital characteristics of NE, DOPAC, 5-HIAA and 5-HT in 10-min-old wounds persisted for up to 12 h at room temperature after death. These results suggest that the HPLC-ECD method used here is very useful for simultaneous examination of the vital reaction in wounds from the earliest to the later stages of the wound-healing period.     

US Defense Innovation and Artificial Intelligence

The United States seeks conventional military overmatch in the strategic competition with China and Russia through efforts to develop advanced technology for military application and new concept of operations as well as pursue organizational adaptation and human talent pool development. The current article will trace the development of US defense innovation efforts from the Obama administration to the Trump administration and focus on the technological dimension of defense innovation by illustrating ways through which the U.S. Department of Defense is attempting to apply “narrow” artificial intelligence for military purposes such as situational awareness, cyber, military logistics, command and control, and swarm technology and tactics for autonomous unmanned systems. The article will conclude by making two caveats regarding the actual introduction of artificial intelligence into the US battle network, and very briefly point to implications for US allies.     

Can the Pivotal mechanism induce truth-telling? An experimental study

In this paper we use a laboratory experiment toexamine the Pivotal mechanism when applied to a binarydecision on a public project of a fixed size. Wefirst point out that the well-known incentivecompatibility of the Pivotal mechanism is true only in aweak sense; There are always strategies otherthan truth-telling that do no worse for a subject thantruth-telling, in any particular set of circumstances.This weakness of the incentive compatibility ofthe Pivotal mechanism makes it difficult for subjects tounderstand that truth-telling is the unique dominantstrategy for the mechanism, unless they havecomprehensive understanding of the payoff structure,with the result that subjects often do not play thedominant strategy. We suggest that this tendency todepart from the dominant strategy can be overcome byproviding subjects with more information about the payoffstructure. We controlled the level of informationprovision in the laboratory and verified that thestrategies used are closer to the dominant strategywhen more information is provided. Under noinformation provision conditions were outcomes poor,but under ``wide'' provision conditions, in which eachsubject experienced a variety of true personalvaluations of the project, departure from thedominant strategy was smaller in magnitude, and under``deep'' provision conditions, in which detailed payofftables were available to each subject, the rate of useof the dominant strategy increased significantly.     

US Technological Competition with China: The Military,Industrial and Digital Network Dimensions

The US approach toward China shifted from engagement to competition, and it has coincided with the near-simultaneous breakthrough of advanced technologies in a whole host of areas ranging from artificial intelligence to synthetic biology. As a result, the United States is competing with China to apply these advanced technologies for military and industrial purposes, and, at the same time, competing in third countries over dominance in digital network infrastructure. The current article will illustrate Chinese efforts and US countermeasures that are unfolding in the form of military technological competition, industrial technological competition, and the digital network competition in order to argue that the US shift toward competition is not only about Chinese discriminatory and unreasonable acts, policies and practices related to technology transfer and cyber intrusions, but a contest for supremacy over the next generation of military, economic and information/data dominance that could impact the relative legitimacy of political and socio-economic system of the two states.     

National institutional response to climate change and stakeholder participation: a comparative study for Asia

Asian countries are building domestic institutions in response to climate change at both the international and domestic levels. Many countries have adopted the inter-agency coordination mechanism (IACM) as a national institutional approach to adjust the functions of various governmental agencies to tackle climate change. This article compares and contrasts national IACMs in four countries: Japan, the Republic of Korea, China and India. It examines (1) the structure and function of an IACM; (2) agencies and their specific roles in IACMs; (3) measures to empower the participation of other stakeholders including local governments, private sector, civil society and academia; and (4) changes in IACMs and reasons for such changes. Four success factors drawn from our comparative study suggest that an effective IACM should feature (1) strong overall coordination by the President/Prime Minister; (2) empowerment of the industry and environment agencies as joint lead agencies coordinating mitigation and adaptation; (3) involvement of all major sectoral agencies related to mitigation and adaptation; and (4) maximisation of the use of the comparative advantages of other stakeholders. The article highlights successful practices in Asia that can be emulated by other governments considering reform of their own domestic institutions in response to climate change.     

Nakasone Yasuhiro’s “Autonomous Defense” and the Security of Japan

Former Prime Minister of Japan Nakasone Yasuhiro advocated autonomous defense throughout the post-WWII period. Nakasone’s concept of autonomous defense (jishu boei) went beyond the idea of enhancing national defense capabilities—it was accompanied by a rich and varied internationalism that strove toward assuaging neighboring countries’ concerns toward Japan’s remilitarization. Nakasone also actively engaged major western powers in the global debate over nuclear issues during his term as prime minister, and it went beyond the confines of Japan’s bilateral security relationship with the United States. Thus, Nakasone’s autonomous defense concept reflected both the development of postwar Japan and the many turbulent changes in the postwar global security landscape. This essay follows the evolution of Nakasone’s autonomous defense concept during his political career from 1950 to the end of his premiership in 1988 and concludes with an overall assessment of his initiatives regarding Japanese security.     

Development of latent fingerprints on metallic surfaces using electropolymerization processes.

We propose a new process for developing latent fingerprints on metal items, applicable to unfired weapons made of Ergal in particular. The method is based on the presence of fatty acids that are contained in fingerprints and act as an insulator on the surface where fingerprints are to be developed. The process of polymerization occurs on the metal portions left untouched by finger contact. Hence, the developing process results as a negative pattern of the original fingerprint. The reaction consists in the electropolymerization of pyrrole and substituted porphyrins, i.e., tetra (o-aminophenyl) porphyrine: radical-cations are generated on superficial nucleation sites by oxidation of monomer, close to the electrode surface; subsequently, the radical species react with the neutral monomer, which begins to diffuse to the electrode. We have also studied the polymer's morphology by means of SEM and AFM, in order to find a correlation between the reagent to be used and the quality of the enhancement process. These are only preliminary results; however, they show that the suggested method is a new way to increase the rate of success in developing latent fingerprints on metal surfaces. In this regard, it may be considered complementary to other conventional procedures, due to the low costs of the instruments and reagents, and the rapidity and simplicity of the treatment.     

Pierre Bayle, the Rights of the Conscience, the "Remedy" of Toleration

Pierre Bayle (1647–1706) is often considered one of the staunchest defenders of toleration, especially in the domain of religion. His Commentaire philosophique , published in 1686, one year after the revocation of the Edict of Nantes, argued for a broad idea of toleration, to be extended with no exceptions to all sects and religions. However, his thought can hardly be reduced to an exaltation of the "rights of the conscience," for he realized very soon that such an exaltation risks bringing forth religious fanatism, which in turn is the cause of religious wars and acts of violence. Toleration, in these conditions, is only a political remedy for the sickness of the human mind.