An innovative transfer DNA experimental design and qPCR assay: Protocol and pilot study

Forensic “touch” DNA samples are low-quantity samples that are recovered from surfaces that have been touched by single or multiple individuals. These samples can include DNA from primary contributors who directly touched the surface, as well as secondary contributors whose DNA was transferred to the surface through an intermediary. It is difficult to determine the type of transfer, or how often and under what conditions DNA transfer occurs. In this paper, we present an innovative protocol that combines (1) a paired male and female transfer DNA experimental design in which the presence of male DNA indicates secondary transfer and (2) a cost-effective quantitative PCR (qPCR) assay of a sex-specific region in the Amelogenin gene to detect male and female DNA. We evaluate the ability of the Amelogenin qPCR assay to detect low concentrations of male and female DNA in mixed samples. We also test experimental DNA samples using our transfer DNA protocol to differentiate primary and secondary DNA transfer. Male DNA was detected in the majority of known mixed samples, even in samples with 4× more female DNA—this result demonstrates the ability to detect low concentrations of male DNA and the presence of secondary transfer DNA in our experimental design. Primary DNA transfer was detected in 100% of our experimental trials and secondary DNA transfer was detected in 37.5% of trials. Our innovative protocol mimics realistic case scenarios to establish rates of primary and secondary DNA transfer in an inexpensive and simplified manner.     

Comment on the Marshall-Hoare-Henssge model for estimating the time since death

Timewise temperature variations in objects that are undergoing unsteady heating or cooling is a commonly encountered problem in the thermal sciences. One particular area of application is the cooling of a body post-death and the use of body temperatures to estimate the time of death. Here, a new approach based on the theory of transient heat transfer is formulated to allow efficient calculation of unsteady conduction problems. The theoretically derived unsteady temperature models are compared with experimentally based correlations (the Marshall-Hoare-Henssge model). The two approaches are found to agree very well. With this new theoretically based approach, timewise temperature variation can be calculated for both large and small Biot number transient problems.     

Heat and let dye: Part II – Rapid heat-fixing of blood traces

Blood-contaminated shoeprints and footmarks contain valuable operational information as they may bind an individual who stepped in the crime scene with the incident and not merely with the location. As determining the age of a bloodstain remains a challenge, while processing the scene, it is difficult to determine whether the blood is completely, or partially, dry. Thus, executing a dye staining protocol may wash these marks away as they might still be soluble. However, to meet this challenge, it is possible to fix blood marks using heat. This study aims to find a solution for floor surfaces covered by heavier blood traces (shoeprints and footmarks). For this purpose, a new pseudo-operating device was constructed for examining the blood-fixing process of both mentioned trace types. Two trials were performed with depletion marks. The results revealed that fully developed fresh and heavily blood deposits were obtained by heating to 200°C for 7.5 min using the fixing device, followed by a staining protocol using amido black solution. The achieved sharp resolution of the examined bloody prints demonstrates that in certain cases the dehydration mechanism of heating is preferred over precipitating the proteins attributed to 5-sulfosalycilic acid; thus, reducing the risk of washing blood evidence while processing the crime scene.     

Justification, perception of severity and harm, and criminalization of wife abuse in the Palestinian society

The purpose of this study was to examine the perceptions of Palestinian adults toward different dimensions of wife abuse. A cross-sectional survey, using a combination of self-administered questionnaires and interviews, was conducted among a systematic random sample of 624 adult Palestinian men and women from the West Bank and Gaza Strip (18 years or older). Study results indicated a strong tendency to justify wife beating in different situations, such as when the wife is perceived as having an affair with another man or as physically attacking her husband. Participants considered the following acts of husband's violence against wife as most severe: using a weapon (86%), having sex with the wife against her will (67%), and hitting her with his fist (57%). The majority of participants thought that wife beating should be considered a crime (82.3%). Traditional marital role expectations was the main significant predictor for all of the study criterion variables. Gender, place of residence, age, and marital status were significant predictors of some of the criterion variables.     

基于胜任力的测查式公务员培训新探——以湖北省省直机关处级公务员为例

本研究以湖北省省直机关处级公务员为例,探索构建以胜任力为导向的测查式公务员培训模式。本文运用胜任力理论和素质测评技术,构建省直机关处级公务员胜任力模型,依据胜任力指标对处级公务员能力现状进行测查和分析,探求其实际能力与岗位胜任力之间的差距,在突出科学测查环节基础上确定培训需求,设计多样化的培训方案,以提升公务员胜任力水平从而提升其工作绩效。     

National Council of Juvenile and Family Court Judges Passes a Set of Practice Reform Resolutions

The National Council of Juvenile and Family Court Judges (NCJFCJ) recently passed resolutions and policy statements on how to improve the lives of youth and families involved with juvenile or family courts. These resolutions address the needs of homeless youth and their families, juvenile probation and adolescent development, and the need for independent oversight of youth confinement facilities.     

Prevalence of Spousal Violence and Associated Risk Factors: Facts from Pakistan Demographics and Health Survey 2012–13

The current study aims to investigate the risk factors associated with the prevalence of spousal violence among the women from 15 to 49 years of age in Pakistan in their marital relationship. Secondary data collected in Pakistan Demographic and Health Survey (PDHS) 2012–2013 is used. In the survey, respondents were selected using probability sampling technique from all the four provinces of Pakistan. Modified and shortened version of Conflict Tactics Scale is used to measure physical and psychological spousal violence among the women perpetuated by their ever husbands. Prevalence of physical, psychological, any type of spousal violence and associated risk factors were analyzed by unadjusted odd ratios (OR) and adjusted odd ratios (aOR). Education, profession, ethnicity and wealth index are found significant risk factors associated with spousal violence. Odds of experiencing spousal violence were higher among the poorer (aOR 1.700 CI 1.272–2.271) as compared to their richer counterparts. Moreover, the prevalence of spousal violence was found the highest (aOR 2.730 CI 2.162–3.447) in Pushton ethnic group. The study recommends improving the literacy rate and economic well-being of the poorer to address the problem of spousal violence in Pakistan.     

Negative governmentality through fundamental rights: The far side of the European Convention on Human Rights

This essay analyses those statements that mention legal norms in negative terms. Specifically, it analyses those statements that define a legal system by mentioning how legal protection does not work and where legal protection ends, and those statements that identify what rights‐holders do not have to with their legally protected free capacities. This essay argues that these statements address a systemic question. It calls such a dynamic as negative governmentality. The argument proceeds in four steps. It introduces the concept of negative governmentality by arguing that the idea of freedom requires both the positive affirmation of moral agency and the constraining of moral agency (Section 2 ). It then explores how rights constitute freedom by limiting rights or making exceptions to them (Section 3 ). Later, it analyses how rights‐based norms prevent abuse of rights by holders of rights (Section 4 ). Finally, it sees how rights‐based norms constrain the legal guarantor of rights, i.e., a state (Section 5 ). The essay concludes by mentioning the importance of negative governmentality (Section 6 ).     

Distribution of the HLA-DQA1 and polymarker alleles in the Basque population of Spain

HLA-DQA1 and polymarker (LDLR, GYPA, HBGG, D7S8, and GC) genotypic and allelic frequencies are determined for a population sample of 102 unrelated Basque individuals using PCR-based methodology. All six loci met Hardy-Weinberg expectations in at least two of the three analyses performed (HLA-DQA1 failed to meet Hardy-Weinberg requirements in the heterozygote deficiency test). Three linkage analysis programs (GDA, GENEPOP and LINKDOS) detected possible linkage disequilibrium between LDLR and HBGG and results from one (GDA) indicated a possible non-random association between HBGG and HLA-DQA1 as well. Allelic data for the six loci are compared to that previously established for other populations (18 for polymarker alone, 16 for polymarker plus HLA-DQA1) to determine homogeneity between the Basque sample and these groups. According to the results of G-tests based on these loci, the Tadjik, a nomadic Caucasian group from western Asia, and the Basque residents are the only sample populations surveyed that are homogenous with the Basque sample. Phylogenetic analysis places the Basque sample correctly within the Caucasian cluster.